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Isolation of Receptors

Isolation of Receptors

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Published by Sunil
Isolation of Receptors
Isolation of Receptors

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Categories:Types, School Work
Published by: Sunil on Jul 20, 2013
Copyright:Attribution Non-commercial


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by Lee Eun Jin
affinity columns with biotinylated ligands
more systematic proteomics
analysis of GPCR-associated protein complexeswas conducted
using receptor-specific antibodies
(7). However, the
general application of these approaches was limited by the
availability of adequate tools (labeled ligands, antibodies, etc.)
for each GPCR. Later on, isolated intracellular domains were
widely used to identify GPCR-associated proteins either as
bait in yeast two-hybrid screens or to generate affinity matrices
for the purification of interacting proteins from cell extracts
(8 –11). Using the entire C-tail of the 5HT2c receptor
expressed as GST fusion protein, more than 15 proteins have
been identified (12). Furthermore isolated protein-protein interaction
motifs such as PDZ domain recognition motifs of 
GPCRs have been successfully used to identify interacting
partners of the PDZ domain recognition motifs of the 5HT2a,
5HT2c, and 5HT4 receptors (13, 14).
A radioligand is a radioactivelylabeled drug that can associate witha receptor, transporter, enzyme, orany site of interest.
Measuring the rate and extent of binding provides information on thenumber of binding sites, and theiraffinity and accessibility for variousdrugs

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