(1) Outer enamel epithelium (OEE)
t’s a layer that is adjacent to the dental sac , it’s a single layer of cuboidal
cells , priorto enamel formation capillaries in the connective tissue surrounding the enamel organproliferate and protrude toward it to increase vascularity, they also have thin walls toenhance the process of diffusion by which nutrients will be reaching the enamelmatrix.
(2) Stellate reticulum (SR)
-Forms the middle part of enamel organ, filled by a large amount on intracellularsubstance (fluid).- Cells are star-shaped with long processes
reaching all directions; they are connectedwith each other and with the cells of outer enamel epithelium and cells of the stratumintermedium with desmosomes.-I
a resistant and elastic structure so its act as a buffer against physical forces.- It also permits the flow of nutrients to formative cells, as it reduces in thickness.After first layers of dentine form cutting the inner enamel epithelium from its originalsupply which is the dental papilla.
(3) Stratum intermedium (SI)
These are flat to cuboidal in shape arranged in three layers, they are believed to play arole in enamel formation through control of fluid diffusion or by contribution of formative elements or enzymes.
(4) Inner enamel epithelium (IEE)
Derived from the basal cell layer of the oral epithelium, before enamel formationbegins, they become columnar and differentiate into ameloblasts starting at the incisalor cuspal edges .These cells are continuous with the outer enamel epithelium at the border of the widebasal opening of the enamel organ thus, forming the cervical loop.