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Mechanism of Propionibacterium Acne Necrosis by Initiation of Reactive Oxygen Species (ROS) by Porphyrin Absorption

Mechanism of Propionibacterium Acne Necrosis by Initiation of Reactive Oxygen Species (ROS) by Porphyrin Absorption

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Published by Caerwyn Ash
Background and Objectives: This study provides scientific evidence to support the clinical effect of photodynamic therapy for acne clearance using 414 nm LEDs and filtered Intense Pulsed Light (IPL). The Propionibacterium acnes (P. acnes) was viewed using a scanning electron microscope (SEM) offering the potential to visualise the destructive mechanism of free radical generation by porphyrin absorption. The benefits of using light to treat acne vulgaris safely and effectively are well known. This study had two objectives, firstly to show the dose response for various wavelengths and energy, secondly to morphologically show microscopic changes in the bacteria due to the effect of wavelengths of light.

Study Design/Materials and Methods: Cultured P. acnes NCTC 737 (HPA, Salisbury, UK) were diluted to 0.5 McFarland using 2 ml of Butterfield’s phosphate buffer solution. Following further dilution, this suspension was pipetted into a 15 mm well plate for a dosed illumination using four IPL and LED light sources, each with different cut-off filters. After illumination of the sample, the suspension was dispensed onto blood agar plates and incubated at 37ºC for 72 hours under anaerobic conditions. Quantitative assessment was performed by counting the total number of colony forming units (CFUs) on each agar plate. Three controls samples were produced per experiment.

Results: At 2 J/cm2 the short wavelength 400 nm filtered IPL has a 15.6% reduction effect on CFUs compared to 5.7% reduction with a 414 nm LED and a 4.6% reduction with a 530 nm IPL. The unfiltered IPL had a 79% reduction at 2 J/cm2, although this output spectrum of the unfiltered IPL contained a significant amount of DNA-damaging wavelengths below 400 nm.

Conclusions: This study showed the mechanism of photodynamic therapy of Propionibacterium at a cellular level. Therefore, illumination of the endogenous coproporphyrin with blue light (400-420 nm) plays a major role in P. acnes photo-inactivation.
Background and Objectives: This study provides scientific evidence to support the clinical effect of photodynamic therapy for acne clearance using 414 nm LEDs and filtered Intense Pulsed Light (IPL). The Propionibacterium acnes (P. acnes) was viewed using a scanning electron microscope (SEM) offering the potential to visualise the destructive mechanism of free radical generation by porphyrin absorption. The benefits of using light to treat acne vulgaris safely and effectively are well known. This study had two objectives, firstly to show the dose response for various wavelengths and energy, secondly to morphologically show microscopic changes in the bacteria due to the effect of wavelengths of light.

Study Design/Materials and Methods: Cultured P. acnes NCTC 737 (HPA, Salisbury, UK) were diluted to 0.5 McFarland using 2 ml of Butterfield’s phosphate buffer solution. Following further dilution, this suspension was pipetted into a 15 mm well plate for a dosed illumination using four IPL and LED light sources, each with different cut-off filters. After illumination of the sample, the suspension was dispensed onto blood agar plates and incubated at 37ºC for 72 hours under anaerobic conditions. Quantitative assessment was performed by counting the total number of colony forming units (CFUs) on each agar plate. Three controls samples were produced per experiment.

Results: At 2 J/cm2 the short wavelength 400 nm filtered IPL has a 15.6% reduction effect on CFUs compared to 5.7% reduction with a 414 nm LED and a 4.6% reduction with a 530 nm IPL. The unfiltered IPL had a 79% reduction at 2 J/cm2, although this output spectrum of the unfiltered IPL contained a significant amount of DNA-damaging wavelengths below 400 nm.

Conclusions: This study showed the mechanism of photodynamic therapy of Propionibacterium at a cellular level. Therefore, illumination of the endogenous coproporphyrin with blue light (400-420 nm) plays a major role in P. acnes photo-inactivation.

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Categories:Types, Presentations
Published by: Caerwyn Ash on Jul 30, 2013
Copyright:Attribution Non-commercial

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08/06/2013

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Caerwyn Ash PhD
1
, Llinos Harris PhD
2
, Thierry Maffeis PhD
3
,
Marc Clement PhD
1
, Gareth Stockman PhD
1
, Mike Kiernan PhD
1
,
Godfrey Town
4
 
1. CyDen Institute of Light Therapy2. Medical Microbiology & Infectious Diseases, Swansea University3. School of Nanotechnology, Swansea University4. University of Wales, Faculty of Applied Design & Engineering, Swansea Metropolitan, Swansea, SA1 6ED, UK
Mechanism of Propionibacterium AcneNecrosis by initiation of Reactive OxygenSpecies (ROS) by Porphyrin Absorption
 
 
2011 Conference, Dallas Texas
 
Statement of Disclosure
 
The following potential conflict of interestrelationships are germane to my presentation:
Salary,
equipment,
travel expensespaid by CyDen Ltd, Swansea, UK
2011 Conference, Dallas Texas
 
Background
 
Collaboration:
2011 Conference, Dallas Texas

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