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Table Of Contents

Protein Purification
A.1 Protein Precipitation
A.2 Column Chromatography
A.2.1 Ionic Exchange Chromatography
A.2.2 Size-Exclusion Chromatography
A.2.3 Affinity Chromatography
Gel-Filtration Chromatography
Affinity Chromatography
A.2.4 Purification of Recombinant Proteins
A.2.5 Commercially Pre-packed Column Kits
Protein Analysis
B.1 Protein Estimation
B.1.1 Absorbance Assays
B.1.2 Colorimetric Assays
B.2 Commercial Protein Estimation Kits
B.4 SDS-PAGE
B.5 Gel Staining
B.5.1 Coomassie Blue Staining
B.5.2 Silver Staining
B.6 Western Blotting
B.7 Immunoprecipitations
Lipid Analysis
C.1 Lipid Extraction
C.1.2 Folch Extraction
C.1.5 Hexane Extraction for Neutral Lipids
C.2 Thin Layer Chromatography
Mammalian Cell Culture
D.1 Primary Culture
D.1.1 Primary Cell Isolation and Culturing
D.1.2 Splenocyte isolation
D.2 Tissue Culture
D.2.1 Adherent Cells
D.2.2 Suspension Cells
D.2.3 Cell Maintenance
D.3 Cell Count
D.4 MammalianTransfection
D.5 Calcium Phosphate Co-Precipitation
D.6 Electroporation Protocol
D.7 LipofectamineTransfection Protocol
Microscopy
E.1 A typical light microscope
E.1 Poly-L-Lysine Immobilisation
E.3 Immunohistochemistry
F.1 Enzyme Assays
F.1.1 Enzyme Kinetics: Basics
F.1.2 Lipid Kinase Assay
F.1.3 Protein Kinase Assay
F.1.4 ProteinTyrosine Phosphatase Assay
F.1.5 Alkaline Phosphatase Assay
F.1.6 Caspase Assay
F.2 Functional Assays
F.2.1 Apoptosis Assay
F.2.3 Chemotaxis Assay
Autoradiography
Suggested Readings
Abbreviations
Appendices
Appendix 1 Safety Issues
Appendix 2 Buffer Chart
Index
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A Manual for Biochemistry Protocols

A Manual for Biochemistry Protocols

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Published by: I AM DREAMCATCHER on May 27, 2009
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