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Field testing, gene flow assessment and pre-commercial studies on transgenic Solanum tuberosum spp. tuberosum (cv. Spunta) selected for PVY resistance in Argentina

Field testing, gene flow assessment and pre-commercial studies on transgenic Solanum tuberosum spp. tuberosum (cv. Spunta) selected for PVY resistance in Argentina

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Solanum tuberosum ssp. tuberosum (cv.Spunta) was transformed with a chimeric transgene containing the Potato virus Y (PVY) coat protein (CP) sequence. Screening for PVY resistance under greenhouse conditions yielded over 100 independent candidate lines. Successive field testing of selected lines allowed the identification of two genetically stable PVY-resistant lines, SY230 and SY233, which were further evaluated in field trials at different potatoproducing regions in Argentina. In total, more than 2,000 individuals from each line were tested along a 6-year period. While no or negligible PVY infection was observed in the transgenic lines, infection rates of control plants were consistently high and reached levels of up to 70–80%. Parallel field studies were performed in virus-free environments to assess the agronomical performance of the selected lines. Tubers collected from these assays exhibited agronomical traits and biochemical compositions indistinguishable from those of the non-transformed Spunta cultivar. In addition, an interspecific out-crossing trial to determine the magnitude of possible natural gene flow between transgenic line SY233 and its wild relative Solanum chacoense was performed. This trial yielded negative results, suggesting an extremely low probability for such an event to occur.
Solanum tuberosum ssp. tuberosum (cv.Spunta) was transformed with a chimeric transgene containing the Potato virus Y (PVY) coat protein (CP) sequence. Screening for PVY resistance under greenhouse conditions yielded over 100 independent candidate lines. Successive field testing of selected lines allowed the identification of two genetically stable PVY-resistant lines, SY230 and SY233, which were further evaluated in field trials at different potatoproducing regions in Argentina. In total, more than 2,000 individuals from each line were tested along a 6-year period. While no or negligible PVY infection was observed in the transgenic lines, infection rates of control plants were consistently high and reached levels of up to 70–80%. Parallel field studies were performed in virus-free environments to assess the agronomical performance of the selected lines. Tubers collected from these assays exhibited agronomical traits and biochemical compositions indistinguishable from those of the non-transformed Spunta cultivar. In addition, an interspecific out-crossing trial to determine the magnitude of possible natural gene flow between transgenic line SY233 and its wild relative Solanum chacoense was performed. This trial yielded negative results, suggesting an extremely low probability for such an event to occur.

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Categories:Types, Research
Published by: Jorge Luis Alonso Gonzalez on Aug 18, 2013
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Transgenic Research
Associated with the InternationalSociety for Transgenic Technologies(ISTT) ISSN 0962-8819  Transgenic ResDOI 10.1007/s11248-011-9584-9
Field testing, gene flow assessment and pre-commercial studies on transgenic
Solanumtuberosum
spp.
tuberosum
(cv. Spunta)selected for PVY resistance in Argentina
Fernando Bravo-Almonacid, ValeriaRudoy, Bjorn Welin, María EugeniaSegretin, María Cecilia Bedogni, FabianaStolowicz, Marcelo Criscuolo, et al.
 
 1 23
Your article is protected by copyright andall rights are held exclusively by SpringerScience+Business Media B.V.. This e-offprintis for personal use only and shall not be self-archived in electronic repositories. If youwish to self-archive your work, please use theaccepted author’s version for posting to yourown website or your institution’s repository.You may further deposit the accepted author’sversion on a funder’s repository at a funder’srequest, provided it is not made publiclyavailable until 12 months after publication.
 
ORIGINAL PAPER
Field testing, gene flow assessment and pre-commercialstudies on transgenic
Solanum tuberosum
spp.
tuberosum
(cv. Spunta) selected for PVY resistance in Argentina
Fernando Bravo-Almonacid
Valeria Rudoy
Bjorn Welin
Marı´a Eugenia Segretin
Marı´a Cecilia Bedogni
Fabiana Stolowicz
Marcelo Criscuolo
Marcelo Foti
Maximiliano Gomez
Mariana Lo´pez
Germa´n Serino
Silvia Cabral
Cristina Dos Santos
Marcelo Huarte
Alejandro Mentaberry
Received: 2 August 2011/Accepted: 16 December 2011
Ó
Springer Science+Business Media B.V. 2011
Abstract
Solanum tuberosum
ssp.
tuberosum
(cv.Spunta) was transformed with a chimeric transgenecontaining the Potato virus Y (PVY) coat protein (CP)sequence. Screening for PVY resistance under green-house conditions yielded over 100 independent can-didate lines. Successive field testing of selected linesallowed the identification of two genetically stablePVY-resistant lines, SY230 and SY233, which werefurther evaluated in field trials at different potato-producing regions in Argentina. In total, more than2,000 individuals from each line were tested along a6-year period. While no or negligible PVY infectionwas observed in the transgenic lines, infection rates of control plants were consistently high and reachedlevels of up to 70–80%. Parallel field studies wereperformed in virus-free environments to assess theagronomical performance of the selected lines. Tuberscollected from these assays exhibited agronomicaltraits and biochemical compositions indistinguishablefrom those of the non-transformed Spunta cultivar. Inaddition, an interspecific out-crossing trial to deter-mine the magnitude of possible natural gene flowbetween transgenic line SY233 and its wild relative
Solanum chacoense
was performed. This trial yieldednegative results, suggesting an extremely low proba-bility for such an event to occur.
Keywords
Field evaluation
Á
Potato
Á
Spunta
Á
PVY
Á
Coat protein
Á
Transgenic
Á
Resistance
Á
Gene flow
Introduction
Potato is the fourth most important food crop in theworld, with an annual production close to 300 milliontons, more than one-third of which are produced indeveloping countries. In Argentina potato is animportant staple crop reaching an annual productionof 2–2.5 million tons which are mostly sold in thedomestic market. Spunta is the most important potato
Electronic supplementary material
The online version of this article (doi:10.1007/s11248-011-9584-9) containssupplementary material, which is available to authorized users.F. Bravo-Almonacid (
&
)
Á
M. E. Segretin
Á
S. Cabral
Á
A. MentaberryInstituto de Investigaciones en Ingenierı´a Gene´tica yBiologı´a Molecular (CONICET), Vuelta de Obligado2490 (C1428ADN), Buenos Aires, Argentinae-mail: fbravo@dna.uba.arF. Bravo-AlmonacidDepartamento de Ciencia y Tecnologı´a, UniversidadNacional de Quilmes, Bernal, Buenos Aires, ArgentinaV. Rudoy
Á
B. Welin
Á
F. Stolowicz
Á
M. Criscuolo
Á
M. Foti
Á
M. Gomez
Á
M. Lo´pez
Á
G. Serino
Á
C. Dos SantosTecnoplant S.A., Parana 3350 (1636) Olivos, BuenosAires, ArgentinaM. C. Bedogni
Á
M. HuarteINTA Estacion Experimental Agropecuaria, Balcarce,Ruta 226 km 73,5 (7620) Balcarce, Buenos Aires,Argentina
 123
Transgenic ResDOI 10.1007/s11248-011-9584-9

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