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Biological Effects_transgenic Maize

Biological Effects_transgenic Maize

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Published by: RicardoSoulForce on Jun 07, 2009
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05/11/2014

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Biological effectsof transgenic maize NK603xMON810 fedin long term reproduction studies in mice
11. November 2008Dr. A. Velimirov, Dr. C. Binter, Univ. Prof. Dr. J. ZentekScientific contribution (alphabetical order):
N. Cyran, Dr. C. Gülly, Dr. S. Handl, G. Hofstätter, F. Meyer,Dr. M. Skalicky, Prof. Dr. R. Steinborn
 
Biological effects of transgenic maize NK603xMON810 fed in long term reproduction studies in mice
Page 2 of 105Department/Universitätsklinik für Nutztiere und öffentlichesGesundheitswesen in der VeterinärmedizinI n s t i t u t f ü r E r n ä h r u n gUniv. Prof. Dr. med. vet. Jürgen ZentekDr. med. vet. Claudia BinterA- 1210 Wien, Veterinärplatz 1Tel. +43 1 25077/3201, Fax: +43 1 25077/3290www.vu-wien.ac.at/i124Forschungsinstitut für biologischen Landbau – FiBLÖsterreichDr. Alberta VelimirovA -1040 Wien, Theresianumgasse 11/1Tel. +43 1 9076313, Fax. 01 4037050191Info.oesterreich@fibl.org www.fibl.org 
 
Biological effects of transgenic maize NK603xMON810 fed in long term reproduction studies in mice
Page 3 of 105
Contents
ABSTRACT.............................................................................................................................................31.INTRODUCTION..................................................................................................................................32. MATERIAL AND METHODS..............................................................................................................32.1.
 
C
ROP PRODUCTION AND CHOICE OF COMPARATORS
.........................................................................32.2.
 
D
IET
...............................................................................................................................................32.2.1. Diet composition..................................................................................................................32.2.2. Analyses of corn and diets .................................................................................................32.3.
 
A
NIMALS AND HOUSING
...................................................................................................................32.4.
 
M
ULTIGENERATION STUDY
(MGS) ...................................................................................................32.4.1. Performance and reproduction data..................................................................................32.4.2. Organ weights......................................................................................................................32.4.3. Histology...............................................................................................................................32.4.4. Immunohistochemistry........................................................................................................32.4.5. Ultrastructural investigations.............................................................................................32.4.6. Microarray analyses.............................................................................................................32.4.7. q-RT-PCR ..............................................................................................................................32.4.8. miRNA analyses...................................................................................................................32.5.
 
L
IFE TERM STUDY
(LTS)..................................................................................................................32.5.1. Feed intake and body mass survey....................................................................................32.5.2. Survival .................................................................................................................................32.6.
 
R
EPRODUCTIVE
A
SSESSMENT BY
C
ONTINUOUS
B
REEDING
(RACB)..................................................32.6.1. Performance and reproduction...........................................................................................32.6.2. q-RT-PCR ..............................................................................................................................33. RESULTS............................................................................................................................................33.1.
 
D
IET
...............................................................................................................................................33.1.1. Harvest in 2005 (diets for MGS and LTS)...........................................................................33.1.2. Harvest in 2007 (diets for RACB)........................................................................................33.2.
 
M
ULTI
G
ENERATION
S
TUDY
(MGS)..................................................................................................33.2.1. Performance and reproduction...........................................................................................33.2.2. Organ weights......................................................................................................................33.2.3. Histology...............................................................................................................................33.2.4. Immunohistochemistry........................................................................................................33.2.5. Ultrastructural investigation...............................................................................................33.2.6. Microarray analyses.............................................................................................................33.2.7. q-RT-PCR ..............................................................................................................................33.2.8. miRNA profiling....................................................................................................................33.3.
 
L
IFE TERM STUDY
(LTS)..................................................................................................................33.4.
 
R
EPRODUCTIVE
A
SSESSMENT BY
C
ONTINUOUS
B
REEDING
(RACB)..................................................33.4.1. Performance and reproduction...........................................................................................33.4.2. q-RT-PCR ..............................................................................................................................34. DISCUSSION......................................................................................................................................35. CONCLUSION ....................................................................................................................................36. DISKUSSION......................................................................................................................................37. SCHLUSSFOLGERUNG....................................................................................................................38. OVERVIEW OF TABLES....................................................................................................................39. OVERVIEW OF FIGURES..................................................................................................................310. REFERENCE LIST............................................................................................................................3

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