Environmental

Solutions

f
Environmental Solutions from Dionex

Sampl Extraction Separation

es Detection

Data
Analysis
and
Reporting 22427
 ASE®—Accelerated Solvent
Extraction
Automated Sample
 U.S. EPA SW-846  Air samples
Concentration
Method 3545 (A) – PUF filters
– Pesticides and – Quartz filters
herbicides
– XAD resins
– Semivolatiles and
PAHs – Tenax resins
– PCBs – Charcoal
adsorbents
– PCDDs and PCDFs
 Organotin and
– TPH (DRO)
organoarsenic
– Explosives compounds
 BFRs
Choose the ASE that Matches
 Ionic materials
Your Laboratory Productivity Requirements
ASE® with In-Cell Cleanup (ICC) Saves
Time and Labor
Brominated Flame Retardants in Fish Tissue
In-Line Sample Prep Calibration
Check
Without
ICC
With ASE-
Salmon,
ICC
ASE-ICC

Extracts With and Without In-Cell Cleanup of Fish

Tissue
Using Alumina, Silica Gel, and Acidic Silica Gel
Extraction and Sample
(40% H Prep
2SO4)
in One Easy Step
 In-Line Cleanup in ASE® Cell
Extraction and Sample Prep in One
Easy Step
1. Alfalfa—Extraction Only
3,500 2. Alfalfa—Extraction +
Sample Prep Sample

Extract
New

Flow
and

ion
Dispersa
µS Method nt
Alumina
Sorbent
and
2
01 OnGuar
d®
–500 Resin
0 5 10 15 20
Minutes Mixture
Automated Perchlorate Sample Preparation
 Columns for Environmental
Analysis
Acclaim® Columns for
Reversed Phase
Separations

IonPac® Columns
for Ion Exchange
Separations

22427
 Typical Anions Found in Drinking
Water
New AS22 Column
Conceptual
Illustration Unique Peaks: ppm
of Surface
COLUMN 7.00 1. Fluoride 5
Modification 1 3 2. Acetate 20
CHEMIST 6 3. Chloride10
RY 5 8
4 4. Nitrite 15
5. Bromide 25
µS 7 6. Nitrate 25
7. Phosphate
2 40
8. Sulfate 30

–1.00
0 5 10 13
Minutes
 AS22 Column Method 300.0
Inorganic Anions Drinking Water
Baseline Resolution with High
Salt Samples
1 8
0.500 3 11 Peak
ppm
5
µS
2
6
9 10
1. Fluoride 0.84
4 7
2. Formate 0.03
3. Chloride 15.6
–0.200
0 5 10 14 4. Nitrite 0.01
45.0 3 5. Unknown
NQ
6. Chlorate 0.18
µS 5 11 7. Bromide 0.02
1 4 67 10
2 8 9 8. Nitrate 0.89
–5.0 9. Carbonate
0 5 10 14
Minutes NQ
 Cations in Drinking Water Sample
ASTM Method D6919-03*
30 mM
1.00Methanesulfonic Acid
4 5 6 Peaks
2
µS 1. Lithium 0.002
1 3
ppm
0.10 2. Sodium 19.7
0 5 10 15 20 25 3. Ammonium 0.07
48 mM 4. Potassium 0.99
1.00Methanesulfonic
4 6 Acid
2 5 5. Magnesium 7.2
6. Calcium 18.5
µS 1 3
RFIC:
Easy method
0.10 development
0 2 4 6 8 10 12
Minutes Immediate results
*EPA proposed for drinking water and wastewater
 Acclaim Explosives Columns
A Total Solution for SW-846 Method 8330
1. HMX
3 4 Acclaim Explosives E1
1 2. RDX
2 56 7 91011 3. 1,3,5-
8 Trinitrobenzene
121314
4. 1,3-Dinitrobenzene

AU Baseline
3 4 11 5. Nitrobenzene
Resolution!
5 10
7 Acclaim Explosives E2
6. Tetryl
7. 2,4,6-
2
1 6 12 14 9 Trinitrotoluene
13 8 8. 4-Amino-2,6-
dinitrotoluene 9. 2-
0 9 18 27 36 45
Amino-4,6-
Minutes dinitrotoluene
10. 2,6-Dinitrotoluene
 Haloacetic Acids by IC-MS/MS
Method Being Jointly Development by EPA
and Dionex
4800 10 Peaks
3
Direct
4
New
Injecti
1. Chloroacetic acid` 6
ppm
2
Method
on 9
Intensity, cps

2. Bromoacetic acid 4
3. Dichloroacetic acid6
4. Bromochloroacetic acid
4
5. Dibromoacetic acid2
1 5 6 8 6. Bromide 2
7
7. Trichloroacetic acid2
8. Bromodichloroacetic acid
0
2 4 6 8 101214161820222426283032343638 2
Minutes 9. Chlorodibromoacetic acid
No Derivatization—No Sample 10 Prep
 Novel LC Separation of
Nitrosamines
220 for
1 EPA 8270 Using Acclaim PA
®

4 56
7 Accurate Analysis of
mAu

23 89 Thermally Labile
Compounds!!
1. N-nitroso-
0 dimethylamine
0 30 2. N-nitroso-
morpholine
1,200,000 ESI Overlay 8 3. N-nitroso-
Intensit

4 methylethylamine
7 4. N-nitroso-
y

6 9
1 23 5
pyrrolidine
0 5. N-nitroso-
2.2 Minutes 11.4 diethylamine
6. N-nitroso- 20109
 Derivatives Using
Coupled Acclaim® PA and Acclaim® C18
Columns
1 Couple Two DNPH derivatives of:
Columns: 1. Formaldehyde
 More plates 2. Acetaldehyde
3. Acrolein
Couple Different 4. Acetone
Columns: 5. Propionaldehyde
2 6. Crotonaldehyde
mAU 4 Improved13
8 1112
3 selectivity 7. Methacrolein
5 8. Butyraldehyde
6 10 9. Methyl ethyl ketone
79
10. Benzaldehyde
11. Valeraldehyde
12. m-Tolualdehyde
0 4 8 12 16 20 24 28 3235 13. Hexaldehyde
Minutes 20112
 Acclaim® Surfactant Column
Separates
a Wide Variety of Surfactants!
Peaks:
1. Chloride
2. Bromide
6 3. Nitrate
5 4. Xylene sulfonate
10 5. Laurylpyridinium
3 chloride
mV 2 6.
4 Lauryldimethylbenzyl-
89 13
1 12 NH4Cl
7. Triton X-100
11 14
8. Cetyl betaine
9. Decyl sulfate
0 5 10 15 20 25 30 35 10. Dodecyl sulfate
Minutes 11. C10–LAS
12. C –LAS
Awards Winning Systems from
Dionex!
Dionex Ion Chromatography
Systems
ICS-3000
ICS-2100 Flexible, dual
ICS-1600 RFIC™ system
Proportioned
ICS-1100 and RFIC™
Dedicated, gradient
ICS-900 Standard single RFIC™ system
integrated system
design Multiple
RFIC™ detectors
Basic Electrolyti gradient
integrat c system System
ed suppressio Electrolytic controller
Entry- design n suppression Multiple
level Electroly LCD front LCD front thermal
integrate tic panel panel zones
d design suppres Column Column Integrated
Chemical sion heater Heater Automation
suppressi
on
A Family of IC and RFIC™ Systems
 Innovative Eluent Generation
Technology
EluGen® KOH
[+] Pt Anode Cartridge
V
(H2O 2H + ½O2 +
+ ent
Current
Innovati
2e–) K+
Electrol
[KOH]
Flow Rate
ve yte
Reserv
K+
Technolo
KOH oir Cation Exchange
Pump Generati Connector
on gy O2
Chambe
H2O r KOH + H2 Degas KOH
Unit
CR-CTC Cation
[ – Pt Trap
Cathode
(2H2O + 2e –
2OH– +
HO )
] 14538-06
 RFIC® Systems Let You Take
Advantage
of Hydroxide Eluents
Concentration (µg/L) 2.2 Chlorite
2.0 Bromate
1.8 Bromate-PCR
1.6 Chlorate
1.4 Bromide
1.2
1.0 Lower MDLs
0.8 Using
0.6 Hydroxide
0.4 Eluent!
0.2
0.0
300.1 317 326 300.1 317 326
AS19 (Hydroxide Eluent)
AS9-HC (Carbonate Eluent)
 Carbonate Removal Device (CRD)
Regen In Regen Out
(Base from CO 3
2– (To CR-TC)
Suppressor) (Carbonate)

Innovati
To the CO (gas) Eluent
Eluent 2 From Eluent
OutCO (gas) In
Conductivity Cell
Inlet Port
ve Regen
2

Regen
Out Port of the
Suppressor

Technolo In
CO
Out
Sample Volume
3
2–

(Carbon  Values:
 Innovation:
gy
– Permeable membrane
ate)
– Minimizes interference
device from
reduces carbonate carbon dioxide in samples
– No reagents required – Improves sensitivity for
– No mechanical pumps trace analysis
required
Improved Sensitivity for – Improves
Trace peak integration
Anion
and quantitation of anions
22280
 Low-Level Perchlorate in Drinking
Water
With and Without Carbonate Removal
1 Column: IonPac AG20, AS20 2 mm
Device ®

Eluent:
100.00 0.5 mM NaOH 0–12 min
Method 65 mM 12.1–28 mm, 100 mM
28.1–35 min
314.1 65.00
Eluent Source: EGC NaOH with CR-ATC
Temperature: 35 °C
µS Flow
B: Without CRDRate: 0.25 mL/min
Inj. Volume:
2 2 mL
1 Rinse Volume: 1 mL (10 mM NaOH)
Concentration: IonPac Cryptand C1 (4 ×
1 Detection: ASRS® ULTRA II, 2 mm
Concentration: 0.50 mM 2
A: With CRD
AutoSuppression external water mode
0
0 5 10 15 20 25 30 35
Peaks: 1. Perchlorate 0.5 µg/L (ppb)
Minutes 2. Unknown —
Improved Sensitivity for Trace Perchlorate 22685
 Perchlorate EPA Method 332.0
(IC-MS)*
18,000 4 Column: IonPac® AG16, AS16, 2-
mm i.d.
Suppressor: ASRS® ULTRA, 2 mm
Conductivity Eluent: 65 mM KOH (EG40)
Flow Rate: 0.30 mL/min
SIM 99 µS Inj. Volume: 250 µL
Counts SIM 99 Detection: 1. Conductivity
Method
SIM 101
332.0
CIO35 –
2. MSQ, SIM 99,
3. MSQ, SIM 101,
4

0 0 CIO
37
4
–

5 7.5 10 MS Conditions: –ESI, 70 V, 350 °C

Minutes
Sample: Groundwater diluted
* Method Developed Jointly by EPA-OGWDW and
1/10
 EPA Perchlorate Method 332.0*
IC-MS/MS
9830 Internal
Peak Area = 2.04 x e5
Counts
Method
332.0
Counts/sec

Standard IC System:
MRM Dionex ICS 2500
107.0/89.0 with Matrix Diversion
0 35
Cl18O3 and Auxiliary Pump
1 µg/L (ppb) Peak Area = 3.16 x e3 MS/MS System:
150 MRM 100.9/84.8 Counts
MDS Sciex API 2000
Counts/sec

37
Cl16O3 Internal Standard:
5 ng/L (ppt) 0–18 Perchlorate: 1
0 µg/L*
Peak Area = 1.00 x e4 Inject Volume:
485 MRM 98.9/82.8 Counts
100 µL
Counts/sec

35
Cl16O3 Perchlorate Isotope Ratio:
5 ng/L (ppt) 10,000/3160 = 3.16
0 Minutes * Prepared from 1 mg/L
2 4 6 8 10 12 14 16
stock standard (Dionex
* Method Developed Jointly by Corp)
EPA-OGWDW
 EPA Method 331.0* LC-MS/MS
Perchlorate Analysis Using Ion-Exchange
Separation,
Matrix
ConceptualDiversion, and MS/MS Detection
Illustration Eluent: 200 mM methyl
LC-MS/MS
of Surface amine
Modification
Flow Rate: 0.35 mL/min
Analysis Column:IonPac®
AS21, 2 mm
IonPac® AS21 Diversion: Eluent divert
valve
Method
Detector: Triple quadrupole
MS/MS 331.0
* Method developed jointly by EPA-
OGWDW and Dionex
 EPA Method 300.1 (B) for Bromate in
(A) Unspiked 8 Drinking Water
0
.75 910, 11 (A) (B)
1 5 7

µS
New ppm ppm
2 6 AS19 1. Fluoride 0.09
–0.05 Column
(B) Spiked 8
2. Formate —
0.75 5 7 3. Chlorite —
RFIC:
1
4
910, 11
Lower
0.020
4. Bromate —
µS
234 6 backgro 0.005 5. Chloride
und 72.7 6.
–0.05
Nitrite 0.090
0 5 10 15 20 25 30 Lower 7. Chlorate 0.120
Minutes
MDL 0.270 8. Bromide
2-D IC Is Easy with the ICS-3000
System
Automation Dual Pump
Manager

Auto Sampler

Eluent Generator

DC Module
Automated with Chromeleon Software
 2-D IC Removes Interfering Matrix
AS20 and AS16 Chemistry
1.35
Combination
A. First Dimension
A. Column: IonPac® AG20,
High AS20, 4 mm
µS Salt Matrix: ppm
Chloride: 1000
Matrix Bicarbonate: 1000
0.75 Sulfate: 1000
1.00
B. Second Dimension
Column: IonPac AG16,
µS B. AS16, 2 mm
Resolved
1 Peak:
Second 1. Perchlorate
Peak
0.5 ug/L
0.40 Dimension
0 10 20 30 40 Resolves
peak
Minutes
Enhances
Introducing UltiMate 3000 Systems

Standard, Micro, and Semiprep LC

New
LC Systems

A New Family of Environmental HPLC Systems

 The UltiMate x2 Dual-Gradient
LC System Solvent Rack
with
6-Channel
Unique Dual-Gradient
Degasser
Pump: Two Ternary
Pumps in
New Autosampler with

LC Systems
One Housing
Optional
Thermostatting
Variable
Wavelength or
Photodiode Array
Column
Detector
Compartment with
Up to Two Switching
Valves

The Ultimate for Environmental Analysis

 Detection of
Different Compounds from One
3,000 Injection Peaks:
2
1.
Uracil 0.675
Scan 2. Naphthalene
Wavelengths 1.617
3. Biphenyl
Improve 3 1.875
mAU Identification 4. Fluorene
6
!1 2.092
4 286 nm 5. Anthracene
3 275 nm 4 2.425
2 250 nm 5
0
1 210 nm 6. Fluoroanthene
2.792
–500
0 0.50 1 1.50 2 2.50 3 3.50 4
Minutes
 Fast LC EPA 604 for Phenols
192
Peaks1. Phenol
2. 2,4- di-NO2-
phenol
3. 4- NO2-phenol
4. 2-Cl-phenol
mAU 5. 2-NO2-phenol
6. 2-Me-4,6-diNO2-
phenol
3X faster with 7. 2,4- di-Me-
phenol
new Acclaim® 8. 4-Cl-3-Me-
Fast LC phenol
–5 Columns 9. 2,4- di-Cl-phenol
0 5 10 15 10. 2,4,6- tri-Cl-
Minutes phenol
11. Penta-Cl-phenol