Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword
Like this
10Activity
0 of .
Results for:
No results containing your search query
P. 1
Maintenance of Column

Maintenance of Column

Ratings: (0)|Views: 3,723|Likes:
Published by sanjeevbhat
Maintenance of HPLC Column
Maintenance of HPLC Column

More info:

Published by: sanjeevbhat on Jun 21, 2009
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PPT, PDF, TXT or read online from Scribd
See more
See less

11/27/2013

pdf

text

original

 
Maintenance of HPLC Column
 
Types Of Column Used In the Laboratory and their  storage solvent 
Reversed Phase: C18, C8, C4, C2, C1,Phenyl ( Methanol)
Normal Phase: Silica, CN, NH
2
, Diol,Alumina (Isopropanol or Hexane)
Ion- Exchange: DEAE, CM, SAX, SEX
Size exclusion: Diol
 
 
Reason for column degradation 
Partially blocked/ plugged frit or columnPartially blocked/ plugged frit or column bed. bed.
Initially poor packed column.Initially poor packed column.
Mechanical or thermal shocks resulting inMechanical or thermal shocks resulting increation of voids (bed subsidence)creation of voids (bed subsidence)
Chemical attack on support/ stationaryChemical attack on support/ stationary phase. phase.
Adsorption of non-eluting compounds etc.Adsorption of non-eluting compounds etc.

Activity (10)

You've already reviewed this. Edit your review.
Hema Chander R liked this
1 thousand reads
1 hundred reads
Bill Hohenschuh liked this
smita379 liked this
mgramesh liked this
chauhansubhash liked this
sandii123 liked this
willson liked this
nayak_saiprasad liked this

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->