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Maintenance of Column

Maintenance of Column

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Published by sanjeevbhat
Maintenance of HPLC Column
Maintenance of HPLC Column

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Published by: sanjeevbhat on Jun 21, 2009
Copyright:Attribution Non-commercial


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Maintenance of HPLC Column
Types Of Column Used In the Laboratory and their  storage solvent 
Reversed Phase: C18, C8, C4, C2, C1,Phenyl ( Methanol)
Normal Phase: Silica, CN, NH
, Diol,Alumina (Isopropanol or Hexane)
Ion- Exchange: DEAE, CM, SAX, SEX
Size exclusion: Diol
Reason for column degradation 
Partially blocked/ plugged frit or columnPartially blocked/ plugged frit or column bed. bed.
Initially poor packed column.Initially poor packed column.
Mechanical or thermal shocks resulting inMechanical or thermal shocks resulting increation of voids (bed subsidence)creation of voids (bed subsidence)
Chemical attack on support/ stationaryChemical attack on support/ stationary phase. phase.
Adsorption of non-eluting compounds etc.Adsorption of non-eluting compounds etc.

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