Scholars Academic Journal of Pharmacy (SAJP) ISSN 2320-4206
Sch. Acad. J. Pharm., 2013; 2(4):293-297
©Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources)www.saspublisher.com
Research ArticleDevelopment and validation of UV-Method for simultaneous estimation of Artesunate and Mefloquine hydrochloride in bulk and marketed formulation
Akshay D. Sapakal*
, Dr. K. A.Wadkar
, Dr. S. K. Mohite
, Dr. C. S. Magdum
Rajarambapu college of pharmacy, Kasegaon, Tal: Walva, Dist: Sangli- Maharashtra (India).
Appasaheb Birnale College of pharmacy, Sangli, Tal & Dist: Sangli- Maharashtra (India).
*Corresponding authorAkshay D.Sapakal
A simple, accurate, precise and economical Uv-method for estimation of Artesunate and Mefloquine HCL wasdeveloped. For analysis of Artesunate and Mefloquine HCl, 242nm and 256nm wavelengths were used respectively. Thelinearity range for Artesunate and Mefloquine HCl were 20-140µg/ml & 50-350µg/ml. Results of analysis were validatedstatistically, recovery studies for ART were 98.33-100.33 & for MEF 101.33-101.92. The LOD and LOQ were found to
be 0.13μg/ml and 0.39μg/m
l for ART. For MEF the LOD and LOQ values were found to be 1.53µg/ml & 2.21µg/ml themethod was validated as per ICH guidelines.
: Artesunate, Mefloquine HCL, UV-Spectroscopy.
ChemicallyART was (3
]-1,2-benzodioxepin-10-ol, hydrogensuccinate  (Fig.1) and chemically MEF was 2- piperidylidyl-2,8-bis (trifluromethyl)-4-quinolinemethanol hydrochloride  (Fig.2) both drugs wereactive against
P.Falciparum and P.vivax
Both of drugs were active against the ring stage of the parasite.Artesunate is ideal for the treatment of severe malaria,including cerebral malaria [3-4].Literature survey reveals High PerformanceLiquid Chromatographic (HPLC)  , ART and MEFestimation by RP-HPLC method and for determinationof ART in human plasma either as single or incombination with other drugs [6-8]. Colorimetricmethod for estimation of ART is also reported withAmodiaquine . No, method has been reported for simultaneous estimation of both drug by UV-Spectrophotometric method. This paper describes asimple, accurate, sensitive and validated UV-Spectrophotometric method for simultaneousquantification of these compounds as the bulk drug andin combined tablet dosage forms. The proposed methodis optimized and validated as per the InternationalConference on Harmonization (ICH) guidelines [9-10].
Fig.no.1 Chemical structure of ARTFig.no.2 Chemical structure of MEFMATERIAL AND METHODSDrugs and Chemicals
A standard drug samples of ART and MEFwere purchased from Zeal Pharma, Mumbai. Allchemicals and solvents used of AR grade. Marketedformulation Falcigo plus tablet containing ART 100mgand MEF 200mg was used.
Standard stock solution of ART and MEF
An accurately weighed quantity of about 10mg of ART & MEF was taken in 10 ml volumetric flask dissolved in sufficient quantity of methanol and dilutedto 10 ml with the same solvent so as to get the
concentration of 1000 μg/ml.
Determination of λ max of ART and MEF
The standard solution of ART was scanned inthe range of 200-
400 nm and the λ max was found to be
242 nm against methanol. Similarly, the standardsolution of MEF was scanned in the range of 200-400
nm and the λ max was found to be 256 nm against