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Molecular Biology

Molecular Biology

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09/18/2010

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Code No: R05222303
Set No. 1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007MOLECULAR BIOLOGY(Bio-Technology)Time: 3 hours Max Marks: 80Answer any FIVE QuestionsAll Questions carry equal marks
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1. What does “right handed helix& “left handed helix” mean? Explain takingsuitable examples. [16]2. How does rolling circle replication produce multimers of a replicon? [16]3. How is the lagging strand synthesis different from the leading strand synthesis inDNA replication? [16]4. Justify the statement “there are different types of rRNA found in ribosome” [16]5. Using suitable examples explain how they are acting as inhibitors of transcription.[16]6. What is meant by “Wobble hypothesis”? Explain its connection to the geneticcode. [16]7. How is the initiation of translation process occurring in prokaryotes and eukaryotes?Elaborate briey. [16]8. Write short notes on [2
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8 = 16](a) Thiamine dimmers and photo reactivation(b) Excision type of DNA repair
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Code No: R05222303
Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007MOLECULAR BIOLOGY(Bio-Technology)Time: 3 hours Max Marks: 80Answer any FIVE QuestionsAll Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. What does “right handed helix” & “left handed helix” mean? Explain takingsuitable examples. [16]2. Using neat diagrams explain a circular chromosome undergoing bi-directional semiconservative replication. [16]3. Explain the process of DNA replication in detail. [16]4. Discuss in detail the structure of the organelles functioning as sites of proteinsynthesis. [16]5. Using suitable examples explain how they are acting as inhibitors of transcription.[16]6. In the genetic code, how can one justify the presence of 64 codons coding for only20 amino acids. [16]7. Once a protein is synthesized, can it be modified? Justify your answer givingsuitable examples. [16]8. With the help of a neat diagram explain how the process of site directed mutagenesisis done. [16]
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Code No: R05222303
Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007MOLECULAR BIOLOGY(Bio-Technology)Time: 3 hours Max Marks: 80Answer any FIVE QuestionsAll Questions carry equal marks
⋆ ⋆ ⋆ ⋆ ⋆
1. Write a note on the different forms of the double helix DNA and elaborate on “B”form. [16]2. How does the bacteriophage Φ
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174 replicate through rolling circle mechanism?[16]3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of telomase in it. [16]4. Write about the dierent types of ribonucleic acids. [16]5. Where does the synthesis & processing of pre-rRNA occur? Explain the process.[16]6. Write briefly about the steps of initiation, elongation and termination in the processof prokaryotic translation. [16]7. What are post transnational modifications? Give some examples. [16]8. How is reverse genetics approach used to study a gene function? [16]
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