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An Introduction to Ultraviolet Visible

An Introduction to Ultraviolet Visible

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Published by Raju Goud Madagoni

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Published by: Raju Goud Madagoni on Oct 01, 2013
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10/01/2013

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 An Introduction toUltraviolet/VisibleMolecular AbsorptionSpectrometry
Haiying LiuDepartment of ChemistryMichigan Technological UniversityHoughton, MI 49931
 
Measurement of Transmittance and Absorbance
Transmittance (T)
Where: P
0
= Power of incident beam,P= Power of emergent beam
100%T  and  
×
0
 P  P 
 
Measurement of Transmittance and Absorbance
In reality, we do not measure the true P
0
. We measure P
Solvent
, thepower of the beam passing through a cell containing solvent only.
 Absorbance (A)
Solvent Solution
 P  P 
=
 P  P  P  P  A
SolutionSolvent Solvent Solution
1loglogloglog
=

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