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*Course Outline of Principle of Cell and Tissue Culture

*Course Outline of Principle of Cell and Tissue Culture

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INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA COURSE OUTLINE
INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA COURSE OUTLINE

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Published by: Biotechnology IIUM Kuantan on Jul 23, 2009
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INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA
COURSE OUTLINE
Kulliyyah
Science
Department
Biotechnology
Programme
Bachelor of Biotechnology (BBT)
Course Title
Principles of Cell and Tissue Culture
Course Code
SBT 2131
Status
Core Course
Level
2
Credit Hours
3
Contact Hours
5 (2 lecture hours and laboratory hours per week)
Pre-requisites
(if any) Nil
Co-requisites
(if any) Nil
InstructionalStrategies
The instructional strategies are:-1)Lecture2)Lab practical3)Quiz4)Presentation
CourseAssessment
State weightageof each type of assessment.
LOMethod%
1,2,4,51,3,1,2,4,51,2,4,5QuizzesLaboratory reportsMid-term examinationFinal Examination
 TOTAL
15152545
100Instructor(s)
Dr. Huzwah Khaza’aiTelephone No.: 03- 6196 5449email:huzwah@iiu.edu.my
SemesterOffered
Semester I
1
 
Course Synopsis
The history of tissue culture. Laboratory requirement, organization andgeneral techniques, animal cell culture; tissue culture media and preparation. Plant cell culture; Zygotic embryo culture; callus and free cellculture; evidence for totipotency; somatic embryogenesis; organogenesis;anther and pollen culture. Isolation, culture and fusion of protoplasts.Genetic stability and somaclonal variation. Experimental design andquantification of results. Quantification and determination of cell viability,kinetics and cell growth. Microbial contamination and prevention procedures. Establishment and characterization of cell lines.
CourseObjectives
The objectives of the course are to: -1.Introduce the underlying principles of aseptic culture of animal, plantor organs.2.To provide information about equipment, procedures and terminologyof aseptic culture.3.Provide an understanding of the advantages of using cell culturesystem and illustrate the differences between techniques employed in plant and animal cell culture.4.Provide students with an understanding of the benefits and issues of tissue culture to the
ummah
.
LearningOutcomes
By the end of the course the student will be able to: -1.Describe the basis of the main procedures of cell line development,cell engineering, cell banking, cell characterisation and mediaformulation.2.Discuss the physiological responses of animal cells to environmentalconditions in culture vessels, including those arising from nutrientlimitation and adverse hydrodynamic effects, and understand anddescribe the principles of growth, death, metabolism and production.3.Perform techniques pertinent to the growth and maintenance of cellculture and able to perform experiments related to medical and biotechnological processes.4.Describe the methods and technologies for the (large-scale) production of therapeutic proteins, gene therapy vectors and vaccinesin animal cells.5.Illustrate knowledge of the principles of plant genetic engineeringtechnologies, and how they are applied to the production of transgeniccrops.
2
 
Content OutlinesWeeksTopicsTask/Reading
1
Animal Cell Culture
An overview of background, advantages, limitations, termsand definitions, growth of attachment-dependent andsuspended cells; cell lines.Chapters1 & 12[1]2
Biology of Cultured Cell
Culture environment, cell adhesion, cell proliferation,differentiation and evolution of cell line.
Chapters3 & 4
[1]3
Design, Layout and Equipment in Animal Cell Culture
Construction, layout; essential, beneficial and usefulequipment; consumable items.Chapters3 & 4[1]4
Aseptic Technique and Safety
Objectives, element of aseptic environment, sterile handling,general safety, risk assessment, radiation and biohazard.Chapters5 & 6[1]5
Media and Serum-free Media
Physicochemical properties, complete media, serum,selection, advantages and disadvantages of serum free media,replacement of serum, media development.Chapters8 & 9[1]6
Primary Culture and Cell Lines
Type of primary culture cell. Isolation of tissues. Primaryculture.Chapter 11[1]7
Cloning Selection and Molecular Techniques
An overview of suspension cloning, isolation of clones,
in situ
molecular hybridization, production of monoclonalantibodies. Somatic cell fusion and DNA transfer.Chapters13 & 27[1]8
Cryopreservation and Quantification
Preservation, cell banks, transporting cells. Quantification.Chapters19 & 20[1]9
Introductory to plant cell culture
Overview of plant cell culture.Chapters2 & 3[2]10
The principles of aseptic culture in plants
Basic media components for plant cell culture.Chapter 4[2]11Organ, Callus Culture and Cell SuspensionCultureBackground and discussions.Chapters5, 7 & 9[2]
3

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