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PCR

PCR

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Published by JSriv

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Published by: JSriv on Jul 25, 2009
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03/20/2013

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POLYMERASE CHAINREACTION
 
 
The Invention of PCR 
A 'licence' to do molecular  biologyA key central techniquethat has revolutionisedmolecular andconsequently cell biologyInvented by Kary Mullisin 1983.First published accountappeared in 1985.Awarded Nobel Prize for Chemistry in 1993.
 
 
Did He Really Invent PCR?
The basic principle of replicating a piece of DNA using two primers had already beendescribed by Gobind Khorana in 1971:
 – Kleppe
et al.
(1971) J. Mol. Biol. 56, 341-346.
Progress was limited by primer synthesisand polymerase purification issues.Mullis properly exploited amplification.

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