DESIGN CONCEPTS AND LAYOUT OF CELL-CULTURE LAB
Tissue culture lab need to maintain asepsis.
Handling of cultured cells in sterile environment.
Provision must be made to ensure that cultures and culture mediaare maintained free from contaminating micro-organism•The lab should be only for the purpose of TC handling and propagationShould not be used for any other experimental work involving the TC eg.virus infection of TC, cell hybridization
Effects of Contaminant
rapid growing in culture mediakill the animal cellsrelease toxindeplete the nutrient mediumdepress the medium pHFACILITIES NEEDED IN TISSUE CULTURE LAB1.Sterile handling area2.Incubation -incubators3.Preparation area4.Wash up area5.Sterilization6.Storage