Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword
Like this
18Activity
0 of .
Results for:
No results containing your search query
P. 1
Dr Siti Suri Lecture 3 Lab Design and Aseptic Technique

Dr Siti Suri Lecture 3 Lab Design and Aseptic Technique

Ratings:

5.0

(2)
|Views: 1,252 |Likes:
LAB DESIGN AND ASEPTIC TECHNIQUE
LAB DESIGN AND ASEPTIC TECHNIQUE

More info:

Published by: Biotechnology IIUM Kuantan on Jul 27, 2009
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as PPT, PDF, TXT or read online from Scribd
See more
See less

12/22/2012

pdf

text

original

 
DESIGN, CONCEPTS AND LAYOUT OF CELL CULTURE LABSConceptsEffects of contaminantsFacilities in TC labsEquipping the TC labsEssential equipmentBeneficial equipmentASEPTIC TECHNIQUESources of contaminantsType of contaminantsMinimising contaminationpipettingsterile handlingpouringObjectives of aseptic techniqueLaminar flow hood and microbiological safety cabinetTypes and functions of cabinetsImportant points regarding the use of the cabinetsOrganizing the hood for routine workUse of antibiotics
 
 
DESIGN CONCEPTS AND LAYOUT OF CELL-CULTURE LAB
CONCEPT 
Tissue culture lab need to maintain asepsis.
Handling of cultured cells in sterile environment.
Provision must be made to ensure that cultures and culture mediaare maintained free from contaminating micro-organismThe lab should be only for the purpose of TC handling and propagationShould not be used for any other experimental work involving the TC eg.virus infection of TC, cell hybridization
Effects of Contaminant
rapid growing in culture mediakill the animal cellsrelease toxindeplete the nutrient mediumdepress the medium pHFACILITIES NEEDED IN TISSUE CULTURE LAB1.Sterile handling area2.Incubation -incubators3.Preparation area4.Wash up area5.Sterilization6.Storage
 
Sterile handling area with
Minimal movement of people past through the clean areaShould have a proper separation between clean area anddirty operations including waste disposalVinyl flooring with dust proof finishingAir conditioningOther facilities essential to cell culture workLaminar flow cabinetsIncubators (CO2)Inverted microscopeInstrument and equipment benches-centrifuge, pipettesMedia fridges (sterile working solutions only)

Activity (18)

You've already reviewed this. Edit your review.
1 hundred reads
1 thousand reads
windua liked this
Sangeeta Shetty liked this
Zaid Nuaj liked this
Ainun Mokhtar liked this
Ainun Mokhtar liked this
Ainun Mokhtar liked this
jburce liked this

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->