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HISTOPATHOLOGY 
Name __________________________________________ Rating________________________ Schedule _____________________________________ Date of Examination ___________ I. __________1. Schiff reagent is a basic fuchsin which is a mixture of:
A.
periodic acid, rosanilin, and magenta II
B.
sulfonic acid, magenta II and rosalinin
C.
rosanilin, pararosanilin and magenta II
D.
rosanilin, pararosanilin and sulfonic acid __________2. Schiff reagent can be prepared using what method/s?1. Barger and Delamater3. De Tomasi-Coleman2. Itikawa and Oguru4. Magenta and Schiff A. 1 and 3 are correctC. 1,2,3 are correctB. 2 and 4 are correctD. all are correct __________3. In the PAS with diastase method for glycogen demonstration,what is the fixative recommended?A. OrthsB. Helly’sC. BouinsD. Carnoy __________4. The method of choice for glycogen staining:A. PASC. PAS with diastaseB. Best carmineD. langhan’s Iodine __________5. In the Best Carmine method for staining glycogen, thecounterstain used is:A. Cole hematoxylinC. Harris hematoxylinB. Ehrlich’s hematoxylinD. Lillies hematoxylin __________6. In the Best Carmine method for staining glycogen, which of thefollowing is/are added to the stock solution to inhibit any non-specific background carmine staining?1. methanol3. Concentrated ammonia2. potassium carbonate4. Potassium chlorideA. 1 and 3 are correctC. 1,2,3 are correctB. 2 and 4 are correctD. all are correct
 
 __________7. Using Langhan’s iodine method for glycogen, the glycogen isstained:A. bright red colorC. mahogany brownB. blue-blackD. yellow __________8. Which of the following staining methods is not applicable foracid mucopolysaccharides?A. colloidal iron techniqueC. aldehyde fuchsin stainB. uranyl-nitrate-azure methodD. azocarmine stain __________9. The most useful metachromatic dye for acid mucin is:A. azure AC. alcian blueB. toluidine blueD. basic fuchsin _________10. Perl’s reagent is consists of:1. 5% hydrochloric acid3. 5% aqueous potassiumferrocyanide2. 2% hydrochloric acid4. 2% aqueous potassiumferrocyanideA. 1 and 3 are correctC. 1,2,3 are correctB. 2 and 4 are correctD. all are correct _________11. The reagent added to Scharlach R to intensify the resultingcolor and prevents deterioration.A. isopropyl alcoholC. dextrinB. alcoholD. benzoic acid _________12. What is used as solvent for Oil red O and Sudan black?A. 99% isopropanolC. 70% alcoholB. 60% alcoholD. 95% alcohol _________13. Lipids may be demonstrated by osmium tetroxidewhich is alsoused as a fixative. The unsaturated fats and fatty acids reducesthe unstable oxide to for what color?A. redB. greenC. blackD. yellow _________14. In the Nile Blue Sulfate method for fats, which of the followingstatements is correct?A. Its red component, oxazone dissolves phospholipidsB. its blue component is called oxazaneC. the oxazine is basic and reacts with phospholipids and freefatty acids.D. neutral fat is blue and fatty acids are pink _________15. In the histochemical demonstration of free fatty acids by stains,which of the following do not match?A. Weigert’s lithium hematoxylin: FischlerB. rubeanicacid: HolczingerC. dimethylaminobenzylidine rhodamine: OkamotoD. diaminobenzidine: Bodian and Lake _________16. This technique involves simultaneous coupling reaction withsodium B-glycerophosphate as the substrate that is hydrolyzedby the enzyme alkaline phosphatase to produce phosphate ions.A. Wachstein and MeiselC. Gomori
 
B. GlennerD. Krajan _________17. In staining for enzyme activity, which of the following do notmatch?A. Filipe and Lake: acetylcholinesteraseB. Indoxylacetate: esteraseC. Tetrazolium: monoamine oxidaseD. a-napththylacetate: ATPase _________18. This is the most reliable and specific histochemical stainingtechnique for DNA.A. Methyl green-pyronin techniqueC. Feulgen techniqueB. May-GrunwaldD. Weigert Pal _________19. In the methyl green-pyronin method which of the following donot match?A. DNA: greenC. Plasma cell cytoplasm: purpleB. RNA: blue-greenD. lymphocytes: purple _________20. Which of the following fluorescent staining do not match?A. fluorescein: blueC. rhodamine: orange redB. acridineorange: blueD. acriflavine: yellow _________21. Which of the following stains do not match?A. Gomori: reticulinC. Van Gieson: CollagenB. Verhoeff’s: elasticD. Lendrum: muscle _________22. Which of the following is not a stain for collagen?A. Van GiesonC. Krajian Aniline RedB. Masson’s TrichromeD. Azocarmine _________23. Which of the following is a not a stain for elastic fibers?A. Weigert’sC. Taenzer-Unna OrceinB. Verhoeff’sD. Aniline Blue _________24. Which of the following is not a component of MSB technique?A. Martius yellowC. Brilliant crystal scarletB. soluble blue C142780D. 2% aqueous potassiumpermanganate _________25. Which of the following is not a component of phosphotungsticacid hematoxylin staining?A. HCl in absolute alcoholC. sulfuric acidB. Glacial acetic acidD. potassium permanganate ________26. The Highman’s Congo Red method for amyloid is consists of:A. 0.2% congo red in 80% alcoholC. 0.5% congo red in 50%alcoholB. 0.5% congo red in 80% alcoholD. 0.2% congo red in 50%alcohol ________27. The Krajian Amyloid stain uses:A. Erhlich’s hematoxylinC. Cole hematoxylinB. Harris hematoxylinD. PTAH ________28. Thioflavine T stain is used as:A. 1%B. 2%C. 5%D. 10%
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shel_hallares

hi sir!..i am i medtech student and this questionair helps me alot in my review preparing for the broad exam especially histopath. can i do a favor if u can send me this questionair through my e.mail miszhzhai@yahoo.com tnx alot.. godbless

reply02 / 21 / 2011
sanwel

sir your sharing is awesome..I'll be very thankful,if u kindly provide the answers...thanks...my email is:::

reply01 / 03 / 2011
07 / 13 / 2010This doucment made it onto the Rising List!
Lhaine_Vennet_7199

Hi Sir Edwin! Where can i get the answers to these questions? especially Histopath. I'm having a hard time mastering it. Please

reply01 / 30 / 2010
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