Pour plate (Loop dilutions)Table: Numbers of Colonies Formed on Agar Plate with First, Second, and Third LoopDilutionLoop dilutionNumbers of colonies formedFirst4Second0Third 0D.Spread plateTable: Numbers of Colonies Formed on Agar PlateDilution Numbers of coloniesYellow RedTotal *cfu/ml*Water solution (undiluted)150111611610First dilution (10
)294333300Second dilution (10
)3033000Third dilution (10
)0000*cfu/ml: colony-forming units per ml of the original water sample* Some colonies were found to be overlapping each other Discussion:The observations on broth cultures can be explained by the arrangement and mobilitycharacteristics of the two different bacterial species.
usually exist assingle rod shape form and they are mobile by the presence of flagella. Therefore, theBroth culture tends to appear turbid in showing the growth of
usually exist in clusters and they are immobile. Hence,the Broth culture tends to form pellicles. Sediment might also form in the culture with
Confluent growth, no isolatedcolony was observed at the beginning of the streak.Isolated colonies wereobserved to appear at the endof the streak. No isolated colony, onlyconfluent growth was observedat the beginning part of thestreak. Only few isolatedcolonies were observed at theending part of the streak.