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Micro Exp 3 Environ

Micro Exp 3 Environ

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This is the experiment about the bacteria adaptation to different environments... the environmental factors include temperature, pH, sucrose concentration, salt solution concentration, and oxygen relation...
This is the experiment about the bacteria adaptation to different environments... the environmental factors include temperature, pH, sucrose concentration, salt solution concentration, and oxygen relation...

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Published by: Peter Hong Leong Cheah on Aug 02, 2009
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07/16/2013

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Universiti Tunku Abdul Rahman (Kampar Campus)Faculty of Science, Engineering, and TechnologyBachelor of Science (Hons) BiotechnologyYear 2 Semester 1UESB 2142 Laboratory 2A(III) MicrobiologyLecturer: Dr. Teh Yok LanStudent’s Name: Cheah Hong LeongStudent’s ID: 08AIB03788Experiment No. 3Title: Environmental InfluencesDate: July 20, 2009
Title: Environmental InfluencesObjectives: –Examine the effects of temperature on microbial growth of different bacteria. –Examine the effects of different osmotic pressure on microbial growth of different bacteria. –Examine the effects of pH on different bacteria.
 
 – 
Determine whether the bacteria being examined are aerobic, anaerobic,microaerophilic or aerotolerant.Results:All the tables below record the observation made on day 2 and 4 after incubation that’s onJuly 22 and July 24, 2009.Table 1: Effects of Different Temperature on Microbial GrowthBacteriaObservations4
o
CRoom Temperature55
o
C
 Pseudomonasafruginosa
Clear Broth solutionFormation o pellicle and yellow-white sedimentClear Broth solution
 Escherichia coli
Clear Broth solutionFormation of whitesedimentClear Broth solution
Micrococcus luteus
Clear Broth solutionFormation oyellowish sedimentClear Broth solutionTable 2: Effect of Osmotic Pressure on Microbial Growth – Sucrose SolutionBacteriaObservationsSucrose 0.5%Sucrose 15%Sucrose 30%
 Pseudomonasafruginosa
Formation oyellow-whitecoloniesFormation oyellow-whitecoloniesFormation oyellow-whitecolonies
 Escherichia coli
Formation of whitecoloniesFormation of whitecoloniesFormation of whitecolonies
Staphylococcusaureus
Formation oyellow-orangecoloniesFormation oyellow-orangecoloniesFormation oyellow-orangecolonies
 
Table 3: Effect of Osmotic Pressure on Microbial Growth – Sodium Chloride SolutionBacteriaObservations NaCl 0.5%NaCl 15%NaCl 25%
 Pseudomonasafruginosa
Formation oyellow-whitecolonies No colony observedNo colony observed
 Escherichia coli
Formation of whitecolonies No colony observedNo colony observed
Staphylococcusaureus
Formation oyellow-orangecolonies No colony observedNo colony observedTable 4: Effect of pH of Medium on Microbial GrowthBacteriaObservations pH 5pH 7pH 9
 Pseudomonasafruginosa
Formation oyellow-whitecoloniesFormation oyellow-whitecoloniesFormation oyellow-whitecolonies
 Escherichia coli
Formation of whitecoloniesFormation of whitecoloniesFormation of whitecolonies
Staphylococcusaureus
Formation oyellow-orangecoloniesFormation oyellow-orangecoloniesFormation oyellow-orangecoloniesTable 5: Relation of Free Oxygen to Microbial GrowthBacteriaObservations
Staphylococcusaureus
Colonies only found in lower part of the tube
 Escherichia coli
Colonies found throughout the tube
Micrococcus luteus
Colonies only found on the upper part and surface of the tube

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