originally present in the primary culture. The terms finite or continuous are used as prefixes if the status of the culture is known. If not, the term line will suffice. The term"continuous line" replaces the term "established line". In any published description of aculture, one must make every attempt to publish the characterization or history of theculture. If such has already been published, a reference to the original publication must bemade. In obtaining a culture from another laboratory, the proper designation of theculture, as originally named and described, must be maintained and any deviations incultivation from the original must be reported in any publication.
A cell strain is derived either from a primary culture or a cell line by theselection or cloning of cells having specific properties or markers. In describing a cellstrain, its specific features must be defined. The terms
are to be usedas prefixes if the status of the culture is known. If not, the term
will suffice. In any published description of a cell strain, one must make every attempt to publish thecharacterization or history of the strain. If such has already been published, a reference tothe original publication must be made. In obtaining a culture from another laboratory, the proper designation of the culture, as originally named and described, must be maintainedand any deviations in cultivation from the original must be reported in any publication.
Chemically defined medium:
A nutritive solution for culturing cells in which eachcomponent is specifiable and, ideally, is of known chemical structure.
Asexual reproduction of plants that are considered to be geneticallyuniform and originated from a single individual or explant.
In animal cell culture terminology a population of cells derived from a single cell by mitoses. A clone is not necessarily homogeneous and, therefore, the terms
do not indicate homogeneity in a cell population, genetic or otherwise. In plantculture terminology, the term may refer to a culture derived as above or it may refer to agroup of plants propagated only by vegetative and asexual means, all members of whichhave been derived by repeated propagation from a single individual.
The percentage of cells plated (seeded, inoculated) that form a clone.One must be certain that the colonies formed arose from single cells in order to properlyuse this term. (See "Colony forming efficiency")
Colony forming efficiency:
The percentage of cells plated (seeded, inoculated) that forma colony.
The ability of two different genetic defects to compensate for oneanother.
Contact inhibition of locomotion:
A phenomenon characterizing certain cells in whichtwo cells meet, locomotory activity diminishes and the forward motion of one cell over the surface of the other is stopped.
Continuous cell culture:
A culture which is apparently capable of an unlimited number of population doublings; often referred to as an immortal cell culture. Such cells may or may not express the characteristics of in vitro neoplastic or malignant transformation.