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Preface
The methods presented in this document were developed by a workgroup of State and USEPA biologistscalled the Mid-Atlantic Coastal Streams (MACS) Workgroup. They were developed for coastal plainstreams from New Jersey to South Carolina to promote the transfer of data and knowledge between Stateand Federal agencies in this region. The methods may also be applicable to other regions that have lowgradient streams. Testing is encouraged in the application of these methods to areas outside this region.This document establishes standard procedures for collecting biological and physical habitat data in lowgradient nontidal streams of the Middle Atlantic Coastal Plain ecoregion (Figure 1). It includes standardmethods for collecting and processing macroinvertebrate samples and for quantifying habitat quality. Themethods developed by the US Environmental Protection Agency (USEPA) (Plafkin et al. 1989) for highgradient streams (i.e., piedmont, mountain) were modified for use in low gradient streams (i.e., coastal,valley bottom, swamp). Modifications were made to address the unique characteristics of these streamswhile retaining the basic assessment approaches the States have used for many years in high gradientstreams.Low gradient streams typically have velocities less than 0.5 fps and lack riffle habitats. Therefore, thekick-net developed for high gradient streams has been replaced by the dip-net, and a variety of habitatsare sampled rather than a single habitat. The coastal plain is a region where alluvial sediments aredeposited. Those habitat parameters that address excessive sediment deposition in the piedmont region(e.g.,
embeddedness) would assess all coastal plain streams in “poor” condition. The twelve habitatparameters commonly used in the piedmont region were reduced to seven parameters through theelimination of those that addressed sediment deposition.This document provides standard methods for producing quantitative measures (i.e., metrics) of biologicaland physical habitat quality. It includes methods for sample collection, sample processing, datamanagement, calculation of metrics, and quality assurance (QA). Standardized procedures include theuse of the 100-organism subsample, a standard sample size of 100-120 organisms for the calculation ofmetrics, a minimum of genus level taxonomy, and a set of QA objectives. The Workgroup Statespresently use a variety of biological metrics and assessment thresholds in the coastal plain. Future effortsof the Workgroup will focus on the analysis of biological metrics to determine those best suited to the Mid-Atlantic Coastal Plain region.The technical basis for these methods is provided in Appendix B, and includes summaries of the literatureand field testing completed by the Workgroup. The estimated variability associated with the metric dataproduced using these methods is also presented in Appendix B. Appendix A provides sample HabitatAssessment Field Data Sheets.
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