Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Save to My Library
Look up keyword
Like this
41Activity
0 of .
Results for:
No results containing your search query
P. 1
Protocols for Recombinant Dna Isolation

Protocols for Recombinant Dna Isolation

Ratings: (0)|Views: 1,325 |Likes:
Published by sivagurunathan.P
Protocols for Recombinant Dna Isolation
Protocols for Recombinant Dna Isolation

More info:

Published by: sivagurunathan.P on Aug 12, 2009
Copyright:Attribution Non-commercial

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as DOC, PDF, TXT or read online from Scribd
See more
See less

05/17/2014

pdf

text

original

 
PROTOCOLS FOR RECOMBINANT DNA ISOLATION,CLONING & SEQUENCING
edited by: Bruce A. RoeJudy S. Crabtreeand Akbar S. KhanDepartment of Chemistry and BiochemistryThe University of OklahomaNorman, Oklahoma 73019e-mail: broe@ou.eduphone: 405 325-4912fax: 405 325-7762August 5, 1995
A printed version of this protocol book can be obtained from:
"DNA Isolation and Sequencing" (Essential Techniques Series)by Bruce A. Roe, Judy S. Crabtree and Akbar S. KhanPublished by John Wiley & Sons, List Price $49.95ISBN 0-471-97324-0QP625.N89R64 1996John Wiley & Sons
 
PROTOCOLS FOR RECOMBINANT DNA ISOLATION,CLONING, and SEQUENCING
edited by: Bruce A. RoeJudy S. Crabtreeand Akbar S. KhanDepartment of Chemistry and BiochemistryThe University of OklahomaNorman, Oklahoma 73019e-mail: broe@ou.eduphone: 405 325-4912fax: 405 325-7762August 5, 1995
A printed version of this protocol book can be obtained from:
"DNA Isolation and Sequencing" (Essential Techniques Series)by Bruce A. Roe, Judy S. Crabtree and Akbar S. KhanPublished by John Wiley & Sons, List Price $49.95ISBN 0-471-97324-0QP625.N89R64 1996John Wiley & Sons
Introduction
 This manual is a compilation of many of the everyday methods used in the average molecular  biology laboratory, with emphasis on the techniques for large scale DNA sequencing protocolsand DNA sequencing automation techniques. The manual has been written in a protocolformat, with little theoretical discussion. For theory and additional information, users of thismanual are referred back to the original literature, or to other textual manuals such as those published by Maniatis (1) et al. and Glover (2).
 
The following persons are acknowledged for contributing methods and suggestions during theassembly of this manual: Stephanie Chissoe, Sandy Clifton, Dennis Burian, Rick Wilson, Din-Pow Ma, James Wong, Leslie Johnston-Dow, Elaine Mardis, Zhili Wang, Kala Iyer, Steve Toth,Goughay Zhang, Hua Qin Pan and other members of the Roe laboratory, both past and present.1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual.Cold Spring Harbor Laboratory Press, NY, Vol. 1, 2, 3 (1989).2. Glover, D.M. DNA Cloning Volume I: A Practical Approach. IRL Press, Oxford, 1985.
Table of Contents
I. GENERAL METHODS
A. Phenol extraction of DNA samplesB. Concentration of DNA by ethanol precipitationC. Restriction digestionD. Agarose gel electrophoresisE. Elution of DNA fragments from agaroseF. Kinase end-labeling of DNAG. Bacterial cell maintenanceH. Fragment purification on Sephacryl S-500 spin columns
II. RANDOM SUBCLONE GENERATION
A. SonicationB. NebulizationC. Random fragment end-repair, size selection, and phosphorylationD. DNA ligationE. Competent cell preparationF. Bacterial cell transformationG. Microcentrifuge tube transformation
III. METHODS FOR DNA ISOLATION
A. Large scale double-stranded DNA isolationB. Midiprep double-stranded DNA isolationC. Miniprep double-stranded DNA isolationD. Large scale M13RF isolation

Activity (41)

You've already reviewed this. Edit your review.
diore0510 liked this
1 thousand reads
1 hundred reads
fateennadeah liked this
darshowlkat liked this
Sutanu De liked this
Salil Shau liked this
Dinesh Kumar liked this
mohdkhair liked this

You're Reading a Free Preview

Download
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->