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preparación de curvas de calibración

preparación de curvas de calibración

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Published by: jljimenez1969 on Nov 24, 2013
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03/09/2014

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Preparation ofCalibration Curves
A Guide to Best Practice
September 2003Contact Point:Liz PrichardTel: 020 8943 7553Prepared by:Vicki BarwickApproved by:________________________________Date:________________________________
The work described in this report was supported undercontract with the Department of Trade and Industry aspart of the National Measurement System ValidAnalytical Measurement (VAM) ProgrammeMilestone Reference: KT2/1.3LGC/VAM/2003/032
© LGC Limited 2003
 
LGC/VAM/2003/032Page i
Contents
1.Introduction12.The Calibration Process2
2.1Planning the experiments22.2Making the measurements32.3Plotting the results4
2.3.1Evaluating the scatter plot5
2.4Carrying out regression analysis6
2.4.1Assumptions72.4.2Carrying out regression analysis using software7
2.5Evaluating the results of the regression analysis8
2.5.1Plot of the residuals82.5.2Regression statistics9
2.6Using the calibration function to estimate values for test samples142.7Estimating the uncertainty in predicted concentrations142.8Standard error of prediction worked example16
3.Conclusions18Appendix 1: Protocol and results sheet19Appendix 2: Example set of results25Appendix 3: Linear regression equations27

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