LABORATORY FOR EXPERIMENTAL EXPERIMENTAL BOTANY VECNA POT 111 SI-1000 LJUBLJANA, SLOVENIA

http://botanika.biologija.org/exp/

UNIVERSITY OF LJUBLJANA BIOTECHNICAL FACULTY DEPARTMENT OF BIOLOGY

contact email: ales.kladnik@bf.uniales.kladnik@bf.uni-lj.si

PHOSPHATE BUFFERED SALINE (PBS)

10x PBS (sodium-potassium combo), 1 L: 2 g KCl 2.4 g KH2PO4 80 g NaCl 11.45 g Na2HPO4
note: if Na2HPO4 (7H2O), use 21.6g, if Na2HPO4 (12H2O), use 28.8g.

Dissolve everything in approximately 800 ml of dH2O, then fill up to 1000 ml. Autoclave and store at room temperature.

10x PBS, pH 74 (sodium), 100 mL: 7,6 g NaCl (Mw 58.4425 g/mol, M = 1,3 mol/L) 0,99 g Na2HPO4 (Mw 141.959 g/mol, M = 0,07 mol/L) 0,41 g NaH2PO4 x H2O (Mw 137.992 g/mol, M = 0,03 mol/L)

Adjust pH with 1 M NaOH or 1 M HCl. Fill up to 100 ml. Autoclave and store at room temperature.

Calculation of Mw: CHEMIX (http://home.c2i.net/astandne/help_htm/chemix.htm)

Last change: 2007-02-01

1/1