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Table Of Contents

Introduction
Purification Strategies - a simple approach
Guidelines for Protein Purification
Preparation
Before You Start
Sample Extraction and Clarification
Three Phase Purification Strategy
Principles
Selection and Combination of Purification Techniques
Sample Conditioning
Capture
Intermediate Purification
Polishing
Examples of Protein Purification Strategies
Three step purification of a recombinant enzyme
Three step purification of a recombinant antigen binding fragment
Two step purification of a monoclonal antibody
One step purification of an integral membrane protein
Sample Extraction and Clarification Procedures
Principles and standard conditions for purification techniques
Gel Filtration (GF)
Expanded Bed Adsorption (EBA)
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Protein Purification

Protein Purification

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Published by Ngoc Hanh Nguyen
Hand book of protein biotech
Hand book of protein biotech

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Published by: Ngoc Hanh Nguyen on Dec 01, 2013
Copyright:Attribution Non-commercial

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03/09/2015

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