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Activities of Antioxidant and Detoxifying Enzymes in Rats After Lead Exposure

Activities of Antioxidant and Detoxifying Enzymes in Rats After Lead Exposure

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Published by: qfbchio on Aug 26, 2009
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07/22/2010

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Activities of Antioxidant and Detoxifying Enzymes in Rats after Lead Exposure
m. a. alghazal
1
, V. lenártoVá
2
, K. holoVsKá
2
, a. sobeKoVá
2
,m. falis
1
, j. legáth
11
D  pcy, pcy d tcy,
2
D  Cy,bcy d byc, Uvy  Vy mdc, Kšc, svk ruc
 Received April 17, 2007  Accepted June 11, 2008
Abstract
a m. a., V. lv, K. hvk, a. skv, m. f, j. l:acv  ad d Dy ey  r  ld eu. ac V. b2008, 77: 347-354.t ud w udk  v  cvy    d yud du (soD) d dy y u-s- (gst)  d  d. ey cv w dd   v, kdy d    d   cvd 100  d 1000   d c  , cvy,   dk w  18 wk. sc y dcd dc d    d    . ad  d vkd  c   soD cvy   v dkdy    d   u y      . gst cvy dcd  v d    , w  cvy cd   v d   . i kdy,  w d d ud   dc   gst cvy   u u   d vkd  c  cvy y   . tuc cd cv
substances (TBARS), an indicator of oxidative stress, signicanty increased in rats that were
v   d d,   kdy    d      . m y, vd c cud   cy   d d ccuu   d   d    dc c  ros .
 Lead intoxication, superoxide dismutase, glutathione-S-transferase, liver, kidneys, heart 
ld   - c vy  wdy dud   v d cc u  w v  d duc  d   yc, ccd vu dyuc (K 1990; fd d W 1992; Yky  .1997). i cy   w udd d vu c v  ud  . t u cud duc    , dy v u d cv  v y (V d U 1972; ec . 2001). mv, c ud v d    c  dcy   duc  d d d c y   cvy c (ros) (gu d ec 2000; W  . 2001). t c vk dv d  cc cu uc  d,  d Dna. i   d  d u   d   w c  dy v d  cv (ady d o 1999). t y cudud du (soD), c (Cat), u d (gp),  wc w ud   cd   d  wk d  d (mcgwd Dd 1986; bc  . 1993; suw  . 1991).soD  cu vu   d  c   c  c duc.t u  ud du (soD)   ud dc  (o
2-
) wc d y     c  cu y. i      dc d  c ccu d    cv yc. soD k  cc  ud dc  w v d  y      dc  dv  (fdvc1997).
Address for correspondence:
p. i. V lv, pD.D  Cy, by d bcyUvy  Vy mdcKk 73, 041 81 Kšcsvk rucp: +421 915 992 256e-: v@uv.k ://www.vu.c/c-v/cv.
ACTA VET. BRNO 2008, 77: 347–354; doi:10.2754/avb200877030347
 
gu-s- (gst)   kw  vd c  dv. s  dc  c  gst cvy   d u (p-b d ed 1992; D  . 1997). t gst   y  cyc
enzymes invoved in the detoxication of a range of xenobiotic compounds by conjugation
 u wc     c   yc c(hyd pud 1995). bd   v cd  udy w cd u vu  c  soD d gst yc cv   v, kdy d   d      d cud.
Materials and Methods
a  (  uy) w  s, mck d b.asy-u ccy y  d  W  spf, 24 wk d, ucd  lf UpjŠKšc w wd  cc    cy   wk  u. oy   ud    ccy cc cd w d   udy. ty w ud y u   c  18 wk(u 22–24 °C, udy 60–70%, 12  dy d  cyc). m w 412 ± 47  d w 290 ± 19        w ud y. p 100  u (12 d 12 ) w cv 100   d c   d p 1000  u (12 d 12 ) w cv 1000   d c    dk w  18 wk, cvy. tc u (8  d 8 ) w cv y dk w. a  cvd   ddd
ad libitum
. On the ast day of experiment, the animas were sacriced by decapitation. The experiments were
ud y ŠVps sr . 7879/04-220/3.p  u cpc   (v, kdy, ) w wd w  w cd , cu  c dd  5 ·d
-3
t-hC u h 7.8 c 0.15 ·d
-3
KC, 1 ·d
-3
n
2
eDta d2 ·d
-3
gsh u U-tu . h (25% w/v) w cud  105 000 ×
 g 
 60  d u w d  -50 °C u ud  y y.t ud du cvy (soD, eC 1.15.1.1) w dd y u    cyc C duc u / d o
2-
 y  550  (f d Ö
1984). One unit of SOD activity was dened as that amount of enzyme causing 50% inhibition of cytochrome C
duc ud  y cd.gu-s- cvy (gst, eC 2.5.1.18) w ud y  cdu  h d jcy
 
(1981) at 30 °C using the substrate CDNB at a na concentration of 1 mmo·dm
-3
.
 
p cc w ud y  d  bdd (1976), u v u u  dd.scc y cvy w d  U·
-1
 .tu c  uc cd cv uc (tbars) w dd  dcd y gud(1984). tbars c w d  c  535     .ld c w dd y  c  ccy d u pk e a ay100 (Kuc 1995).sct u  v   ± sD. D w yd u sud’
-test with the signicance eve of 
 p
 < 0.05,
 p
< 0.01 d
 p
< 0.001, cvy.
Results
t c  d w dd   v, kdy d     18wk d  100 /  1000 /  d c, cvy. i  c    c  d (/k) w 0.050 ± 0.009   kdy, 0.025 ±0.004   v d 0.017 ± 0.001   , cvy. i  c    d c (/k) w 0.06 ± 0.02   kdy, 0.033 ± 0.006   v d0.020 ± 0.004   , cvy. t d c     c d   d   c  c  t 1. t  c d c w vd   kdy   (70-d) d  (230-d)  p 1000 u. acv   d y soD d dy ygst w dd   ’ cy  v d kdy, d   uc348
 
    c u d   u p 100 d p1000, cvy. sc y
indicated a signicant effect of sex
  y cv  . a
signicant increase of the SOD activity
w vd   v, u p 1000  , w    c w    p100 u.a  kdy,   c   soD cvy ccud   p1000  d  u. W c w vd   soDcvy    , soD cvycd   p 100 d p 1000u     (f. 1).ld  kw  d ducdu,  u  gst.i    v d  
GST activity signicanty decreased in
p 1000 u. i c,  u  gst cvy cd v p 100 u d   p100 d p 1000 u   .
Interesting ndings were determined
  kdy. W  w dd ud   dc   gstcvy  p 100  d u,   d d (p 1000u) vkd  c   gstcvy y    (f. 2).tbars vu,  dc  
oxidative stress, signicanty increased
y  u    d d   kdy d   (f.3). i     d349
t d   ± sDt 1. ld c    ld c  c  c (%) o m fp 100 p 1000 p 100 p 1000lv 250 ± 10 3190 ± 13 359 ± 19 9280 ± 20Kdy 492 ± 15 7000 ± 22 410 ± 22 23000 ± 29h 205 ± 14 379 ± 26 246 ± 12 775 ± 11
Fig. 1. Specic activity of SOD in the iver,
kdy d     v d c.Vu  v  
sD ( = 6). ak
represent signicant differences between the
c u d u v d c(*
 P 
 
0.05, **
 P 
< 0.01, ***
 P 
< 0.001).

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