iLib08 - CitaviiLib08 - CitaviiLib08 - CitaviiLib08 - Citavi Abe, Toshiaki; Saigo, Yoko; Hojo, Masayoshi; Kano, Tetsuya; Wakusawa, Ryosuke; Tokita, Yumi; Tamai,Makoto (2006): Protection of photoreceptor cells from phototoxicity by transplanted retinal pigment epithelialcells expressing different neurotrophic factors. In: Cell transplantation, Jg. 14, H. 10, S. 799–808. AbstractTransplantation of cells or tissues and the intravitreal injection of neurotrophicfactors are two methods that have been used to treat retinal diseases. The purposeof this study was to examine the effects of combining both methods: thetransplantation of retinal pigment epithelial (RPE) cells expressing differentneurotrophic factors. The neutrophic factors were Axokine, brain derived-neurotrophic factor (BDNF), and basic fibroblast growth factor (bFGF). Theenhanced green fluorescence protein (eGFP) gene was used as a reporter gene.These genes were transduced into RPE cells by lipofection, selected by antibiotics,and transplanted into the subretinal space of 108 rats. The rats were examined at 1week and 3 months after the transplantation to determine whether the transducedcells were present, were expressing the protein, and were able to protectphotoreceptors against phototoxicity. The survival of the transplanted cells wasmonitored by the presence of eGFP. The degree of protection was determined bythe thickness of the outer nuclear layer. Our results showed that the degree of photoreceptor protection was different for the different types of neurotrophic factorsat 1 week. After 3 months, the number of surviving transplanted cell was markedlyreduced, and protection was observed only with the BDNF-transduced RPE cells. Asignificant degree of rescue was also observed by BDNF-transduced RPE cells inthe nontransplanted area of the retina at both the early and late times. Lymphocyticinfiltration was not detected in the vitreous, retina, and choroid at any time. Weconclude that the transplantation of BDNF-transduced RPE cells can reduce thephotoreceptor damage induced by phototoxicity in the transplanted area and weaklyin the nontransplanted area.Schlagwörter Animals; Brain-Derived Neurotrophic Factorbiosynthesisgeneticsphysiology; CellSurvival; Cell Transplantation; Ciliary NeurotrophicFactorbiosynthesisgeneticsphysiology; Dermatitis,Phototoxicpathologyphysiopathologyprevention & control; Fibroblast Growth Factor 2biosynthesisgeneticsphysiology; Gene Expression Regulation; Genes, Reporter;Green Fluorescent Proteinsanalysisgenetics; Lightadverse effects; Nerve GrowthFactorsbiosynthesisgeneticsphysiology; Pigment Epithelium of Eyechemistrycytologymetabolismtransplantation; Rats; Rats, Long-Evans; Rats,Sprague-Dawley; Retinal Rod Photoreceptor Cellscytologyphysiology;Transduction, Genetic Albrecht-Buehler, G. (1977): Phagokinetic tracks of 3T3 cells: parallels between the orientation of tracksegments and of cellular structures which contain actin or tubulin. In: Cell, Jg. 12, H. 2, S. 333–339. AbstractPhagokinetic tracks were used to determine the current direction of migration in 3T3cells. Comparing this direction with the orientation of actin or tubulin-containingcellular structures by indirect immunofluorescence, the following results wereobtained. First, the main actin-containing bundles were located at the bottom andtail end of 3T3 cells and ran parallel to the current or preceding direction of migration. Second, the 3 micrometer long rod-like structure (primary cilium), whichcontains tubulin and which has been observed by other investigators intransmission electron microscopy (Barnes, 1961; Sorokin, 1962; Wheatley, 1969)and in indirect immunofluorescence (Osborn and Weber, 1976), was orientedpredominantly parallel to the substrate and to the current movement direction. Itseems possible that the primary cilium has a role in the directional control of amigrating 3T3 cell, and that the main actin containing bundles act as substrate-attached rails along which the nucleus and bulk cytoplasm slide duringdisplacement of the cells.Schlagwörter Actins; Cell Line; Cell Movement; Cilia; Fluorescent Antibody Technique;Glycoproteins; Microtubules; Tubulin

iLib08 - CitaviiLib08 - CitaviiLib08 - CitaviiLib08 - Citavi Albrecht-Buehler, G. (1997): Autofluorescence of live purple bacteria in the near infrared. In: Experimental cellresearch, Jg. 236, H. 1, S. 43–50. Online verfügbar unter doi:10.1006/excr.1996.3688. AbstractWe have developed a novel microscope with which to study the fluorescence of cells in the near-infrared region (lambda = 750-2500 nm). For one of its firstapplications we report on the autofluorescence of live purple bacteria,Rhodospirillum rubrum, and suggest that the autofluorescent component isbacteriochlorophyll. The rapid fading of the autofluorescence of fixed bacteria andof purified bacteriochlorophyll suggests that the live bacteria are able to regeneratetheir pigment with a time constant of approximately 20 s.Schlagwörter Bacteriochlorophylls; Infrared Rays; Microscopy, Fluorescence; Rhodospirillumrubrum; Time Factors Allen, Karen Miller (1983): Artificial lighting and health. A selected bibliography. Monticello Ill.: VanceBibliographies (Architecture series--bibliography, A-1087).SchlagwörterFluorescent lighting; Physiological effect; Bibliography.; Health aspects; Electriclighting Amick, Charles L. (1947): Fluorescent lighting manual. 2nd ed. Fluorescent lighting (Hg.). New York: McGraw-Hill.SchlagwörterFluorescent lighting. Arai, Takao; Tani, Satoshi; Isoshima, Akira; Nagashima, Hiroyasu; Joki, Tatsuhiro; Takahashi-Fujigasaki, Junko; Abe, Toshiaki (2006): [Intraoperative photodynamic diagnosis for spinal ependymoma using 5-aminolevulinicacid: technical note]. In: No shinkei geka. Neurological surgery, Jg. 34, H. 8, S. 811–817. AbstractOBJECTIVE: The fluorescence-guided resection using 5-aminolevulinic acid (5- ALA) is a well established method for the treatment of brain tumor, especiallymalignant glioma. However, there is no report on photodynamic diagnosis (PDD) for spinal tumor. In the present study, we evaluated the usefulness of PDD for spinalependymoma using 5-ALA. METHODS: Three patients with spinal ependymomareceived oral doses of 5-ALA (20 mg/kg body weight) 2 hours before anesthesiainduction. Intraoperatively, fluorescence was observed with a 420 nm sharp cut filter after excitation with a violet semiconductor laser (405 nm) and was verified byanalysis of fluorescent spectra. Residual fluorescent samples taken from the tumor cavity were examined histologically RESULTS: Fluorescence peaked at 636nm inthe removed tumors in all cases. Fluorescent tissue tended to exist at the cranialand caudal portion in the tumor cavity or around the anterior median fissure. Theresidual fluorescent tissue was not detected after removal of the tumor in case 1.The residual fluorescent tissue was composed of tumor cells and ependymal liningin case 2 or the infiltrated inflammatory cells and vascular endothelial cells in case3. Postoperative magnetic resonance (MR) imaging showed no residual tumor inany of the cases. CONCLUSION: The results of this study indicate the usefulnessof 5-ALA-induced tumor fluorescence in guiding resection of spinal ependymoma.5-ALA-induced porphyrin fluorescence may label spinal ependymomas easily andclearly enough to enhance the completeness of tumor removal.Schlagwörter Adult; Aminolevulinic Acid; Ependymoma; Female; Fluorescence; Humans;Intraoperative Period; Lighting; Magnetic Resonance Imaging; Male; Middle Aged;Photosensitizing Agents; Porphyrins; Sensitivity and Specificity; Spinal CordNeoplasms Atkinson, Arthur Dinham Stephen (1944): Fluorescent lighting. Dealing with the principles and practice of fluorescent lighting, for electrical engineers, illuminating engineers and architects. London: Newnes.SchlagwörterFluorescent lighting. Atkinson, Arthur Dinham Stephen (1946): Fluorescent lighting. Brooklyn N.Y.: Chemical publishing co. inc.SchlagwörterFluorescent lighting.

iLib08 - CitaviiLib08 - CitaviiLib08 - CitaviiLib08 - Citavi Atkinson, Arthur Dinham Stephen (1951): Modern fluorescent lighting. Dealing with the principles and practice of fluorescent lighting, for electrical engineers, illuminating engineers, and architects. London: Newnes.SchlagwörterFluorescent lighting. Atkinson, Arthur Dinham Stephen (1955): Modern fluorescent lighting. Dealing with the principles and practice of fluorescent lighting, for electrical engineers, illuminating engineers, and architects. 2d ed. London: Newnes.SchlagwörterFluorescent lighting.Ben-Hur, E.; Elkind, M. M. (1972): Survival response of asynchronous and synchronous Chinese hamster cellsexposed to fluorescent light following 5-bromodeoxyuridine incorporation. In: Mutation research, Jg. 14, H. 2, S.237–245.SchlagwörterAnimals; Bromodeoxyuridinemetabolismpharmacology; Cell Line; Cell Survivaldrugeffectsradiation effects; Cells, Culturedradiation effects; Cricetinae;Cysteaminepharmacology; DNAradiation effects; DNA Replication; Fibroblastsdrugeffectsradiation effects; Fluorescence; Light; Mercaptoethylaminespharmacology;Photic Stimulation; Radiation Dosage; Radiation Effects; Radiation-Protective Agents; Radiation-Sensitizing Agents; Time FactorsBen-Hur, E.; Elkind, M. M. (1972): Damage and repair of DNA in 5-bromodeoxyuridine-labeled Chinese hamster cells exposed to fluorescent light. In: Biophysical journal, Jg. 12, H. 6, S. 636–647. Online verfügbar unter doi:10.1016/S0006-3495(72)86109-5.SchlagwörterAnimals; Bromodeoxyuridinemetabolism; Cell Line; Centrifugation, DensityGradient; Cricetinae; Cysteaminepharmacology; DNAbiosynthesisradiation effects;DNA Repair; Fibroblastsdrug effectsmetabolismradiation effects; Fluorescence;Isotope Labeling; Kinetics; Light; Molecular Weight; Radiation Effects; Radiation-Protective Agents; Thymidinemetabolism; TritiumBen-Hur, E.; Elkind, M. M. (1974): Letter: Damage and repair of DNA in 5-bromodeoxyuridine-labeled Chinesehamster cells exposed to fluorescent light. In: Biophysical journal, Jg. 13, H. 12, S. 1342. Online verfügbar unter doi:10.1016/S0006-3495(73)86067-9.SchlagwörterAnimals; Bromodeoxyuridine; Cell Line; Centrifugation, Density Gradient;Cricetinae; DNA Repair; DNA, Single-Strandedbiosynthesis; Fluorescence;Molecular WeightBeral, V.; Evans, S.; Shaw, H.; Milton, G. (1982): Malignant melanoma and exposure to fluorescent lighting atwork. In: Lancet, Jg. 2, H. 8293, S. 290–293. AbstractIn a study of 274 women with malignant melanoma, aged 18--54 years, and 549matched controls in New South Wales, Australia, reported exposure to fluorescentlight at work was associated with a doubling of melanoma risk (relative risk [RR] =2.1; 95% confidence limits 1.32--3.32). The risk grew with increasing duration of exposure to fluorescent light and was higher in women who had worked mainly inoffices (RR = 2.6) than in women whose main place of work was indoors but not inoffices (RR = 1.8). The findings could not be explained by the differences inhistories of sunlight exposure, in skin or hair colour, or in any other factor. Therewas a relative excess of lesions on the trunk in the group exposed to fluorescentlight at work. 27 men with melanoma and 35 similarly aged controls were studied,and a significant increase in risk was also found: the RB in those exposed for greater than or equal to 10 years compared with those exposed for less than 10years was 4.4 (95% confidence limits 1.1--17.5). Such an association has not beenreported before, but it is plausible and could explain many of the paradoxicalfeatures of the epidemiology of melanoma. Until more data accumulate it must,however, be viewed cautiously.Schlagwörter Adolescent; Adult; Age Factors; Female; Fluorescence; Humans; Lightadverseeffects; Lighting; Melanomaetiology; Middle Aged; Occupational Diseasesetiology;Risk; Sex Factors; Skin Neoplasmsetiology