Description

The contents of the book is as follows:
1. A Short History of the Polymerase Chain Reaction
John M. S. Bartlett and David Stirling

2. PCR Patent Issues
Peter Carroll and David Casimir

3. Equipping and Establishing a PCR Laboratory
Susan McDonagh

4. Quality Control in PCR
David Stirling

5. Extraction of Nucleic Acid Templates
John M. S. Bartlett

6. Extraction of DNA from Whole Blood
John M. S. Bartlett and Anne White

7. DNA Extraction from Tissue
Helen Pearson and David Stirling

8. Extraction of DNA from Microdissected Archival Tissues
James J. Going

9. RNA Extraction from Blood
Helen Pearson

10. RNA Extraction from Frozen Tissue
John M. S. Bartlett

11. RNA Extraction from Tissue Sections
Helen Pearson

12. Dual DNA/RNA Extraction
David Stirling and John M. S. Bartlett

13. DNA Extraction from Fungi, Yeast, and Bacteria
David Stirling

14. Isolation of RNA Viruses from Biological Materials
Susan McDonagh

15. Extraction of Ancient DNA
Wera M. Schmerer
16. DNA Extraction from Plasma and Serum
David Stirling

17. Technical Notes for the Detection of Nucleic Acids
John M. S. Bartlett

18. Technical Notes for the Recovery and Purificationof PCR Products from Acrylamide Gels
David Stirling

19. PCR Primer Design
David L. Hyndman and Masato Mitsuhashi

20. Optimization of Polymerase Chain Reactions
Haiying Grunenwald

21. Subcycling PCR for Long-Distance Amplificationsof Regions with High and Low Guanine–Cystine Content---- Amplification of the Intron 22 Inversion of the FVIII Gene
David Stirling

22. Rapid Amplification of cDNA Ends
Xin Wang and W. Scott Young III
23. Randomly Amplified Polymorphic DNA Fingerprinting--The Basics
Ranil S. Dassanayake and Lakshman P. Samaranayake

24. Microsphere-Based Single NucleotidePolymorphism Genotyping
Marie A. Iannone, J. David Taylor, Jingwen Chen, May-Sung Li,Fei Ye, and Michael P. Weiner

25. Ligase Chain Reaction
William H. Benjamin, Jr., Kim R. Smith, and Ken B. Waites

26. Nested RT-PCR in a Single Closed Tube
Antonio Olmos, Olga Esteban, Edson Bertolini, and Mariano Cambra

27. Direct PCR from Serum
kenji Abe

28. Long PCR Amplification of Large Fragmentsof Viral Genomes
---A Technical Overview
Raymond Tellier, Jens Bukh, Suzanne U. Emerson, and Robert H. Purcell

29. Long PCR Methodology
Raymond Tellier, Jens Bukh, Suzanne U. Emerson, and Robert H. Purcell

30. Qualitative and Quantitative PCR-----A Technical Overview
David Stirling

31. Ultrasensitive PCR Detection of Tumor Cells in Myeloma

Friedrich W. Cremer and Marion Moos
32. Ultrasensitive Quantitative PCR to Detect RNA Viruses

Susan McDonagh
33. Quantitative PCR for cAMP RI Alpha mRNA
---- Use of Site-Directed Mutation and PCR Mimics
John M. S. Bartlett

34. Quantitation of Multiple RNA Species
Ron Kerr

35. Differential Display----- A Technical Overview
John M. S. Bartlett

36. AU-Differential Display, Reproducibilityof a Differential mRNA Display Targeted to AU Motifs
Orlando Dominguez, Lidia Sabater, Yaqoub Ashhab,Eva Belloso, and Ricardo Pujol-Borrell

37. PCR Fluorescence Differential Display
Kostya Khalturin, Sergej Kuznetsov, and Thomas C. G. Bosch

38. Microarray Analysis Using RNA Arbitrarily Primed PCR
Steven Ringquist, Gaelle Rondeau, Rosa-Ana Risques,Takuya Higashiyama, Yi-
Peng Wang, Steffen Porwollik,David Boyle, Michael McClelland, and John Welsh

39. Oligonucleotide Arrays for Genotyping
--- Enzymatic Methods for Typing Single Nucleotide Polymorphisms and Short
Tandem Repeats
Stephen Case-Green, Clare Pritchard, and Edwin Southern

40. Serial Analysis of Gene Expression
Karin A. Oien

41. Mutation and Polymorphism Detection----- A Technical Overview
Joanne Edwards and John M. S. Bartlett
42. Combining Multiplex and Touchdown PCR for Microsatellite Analysis
Kanokporn Rithidech and John J. Dunn
Detection of Microsatellite Instability and Loss