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Macromolecular Syntheses in the Pancreatic Acinar Cells of Aging Mice as Observed by Electron Microscopic Radioautography

Macromolecular Syntheses in the Pancreatic Acinar Cells of Aging Mice as Observed by Electron Microscopic Radioautography

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Published by iMedPub
For the purpose of studying the aging changes of macromolecular syntheses in
the pancreatic acinar cells of experimental animals, we studied 30 groups of aging
mice, during aging from fetal day 19 to postnatal month 24. They were injected
with one of the nucleic acid precursors such as 3H-thymidine for DNA precur-
sor, 3H-uridine for RNA precursor or 3H-leucine for protein precursor, then the
pancreatic tissues were fixed and processed for light and electron microscopic
radioautography. On many radioautograms the localizations of silver grains demon-
strating DNA, RNA and protein syntheses in pancreatic acinar cells were analyzed
qualitatively and quantitatively. The number of mitochondria per cell, the number of labeled mitochondria with
silver grains in respective aging groups were counted. The results revealed that
the nuclei of pancreatic acinar cells, centro-acinar cells, ductal epithelial cells, and
endocrine cells were labeled with 3H-thymidine, 3H-uridine or 3H-leucine, demonstrating nucleic acid or protein syntheses. On the other hand, we also found the
silver grains in mitochondria of various cells not only in the nuclei of such cells as
the livers, kidneys or adrenals showing intramitochondrial DNA, RNA and protein
syntheses. We found that the activities of DNA, RNA and protein syntheses in mi-
tochondria of various cells changed due to aging of individual animals. The number
of mitochondria, the number of labeled mitochondria and the mitochondrial labeling index labeled with silver grains were analyzed in each pancreatic acinar cell.
It was demonstrated that the numbers of mitochondria, the numbers of labeled
mitochondria and the labeling indices in the pancreatic acinar cells showing DNA,
RNA and protein syntheses changed due to aging. The DNA synthetic activity
increased from prenatal day 19 to postnatal day 14, then decreased to month 24,
the RNA synthetic activity increased from embryonic day 19 to postnatal month 6,
then decreased to month 24, while the protein synthetic activity decreased from
prenatal day to postnatal day 1, then increased to postnatal month 2 and decreased
again to month 24. These results demonstrated the aging changes of mitochondrial
macromolecular syntheses in pancreatic acinar cells of mice.
For the purpose of studying the aging changes of macromolecular syntheses in
the pancreatic acinar cells of experimental animals, we studied 30 groups of aging
mice, during aging from fetal day 19 to postnatal month 24. They were injected
with one of the nucleic acid precursors such as 3H-thymidine for DNA precur-
sor, 3H-uridine for RNA precursor or 3H-leucine for protein precursor, then the
pancreatic tissues were fixed and processed for light and electron microscopic
radioautography. On many radioautograms the localizations of silver grains demon-
strating DNA, RNA and protein syntheses in pancreatic acinar cells were analyzed
qualitatively and quantitatively. The number of mitochondria per cell, the number of labeled mitochondria with
silver grains in respective aging groups were counted. The results revealed that
the nuclei of pancreatic acinar cells, centro-acinar cells, ductal epithelial cells, and
endocrine cells were labeled with 3H-thymidine, 3H-uridine or 3H-leucine, demonstrating nucleic acid or protein syntheses. On the other hand, we also found the
silver grains in mitochondria of various cells not only in the nuclei of such cells as
the livers, kidneys or adrenals showing intramitochondrial DNA, RNA and protein
syntheses. We found that the activities of DNA, RNA and protein syntheses in mi-
tochondria of various cells changed due to aging of individual animals. The number
of mitochondria, the number of labeled mitochondria and the mitochondrial labeling index labeled with silver grains were analyzed in each pancreatic acinar cell.
It was demonstrated that the numbers of mitochondria, the numbers of labeled
mitochondria and the labeling indices in the pancreatic acinar cells showing DNA,
RNA and protein syntheses changed due to aging. The DNA synthetic activity
increased from prenatal day 19 to postnatal day 14, then decreased to month 24,
the RNA synthetic activity increased from embryonic day 19 to postnatal month 6,
then decreased to month 24, while the protein synthetic activity decreased from
prenatal day to postnatal day 1, then increased to postnatal month 2 and decreased
again to month 24. These results demonstrated the aging changes of mitochondrial
macromolecular syntheses in pancreatic acinar cells of mice.

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