ACP (Acid phosphate) E.C. 3.1.3.2 Acid orthosphoric monoester phosphydrolase Isoenzyme: Prostatic ACP (pH 4. !".

1) #ed Cell ACP (pH ".$!$.%) &ptim'm pH: 4.( ) ".1 (pH ") *iss'e so'rces: o o o o o o Prostate +land ,eminal -l'id Platelets ,pleen .i/er 0one marro1

2ethod -or Assay: 3'tman and 3'tman ,'4trate: diazoni'm phenyl phosphate 0'--er: citrate (pH4.() 2eas're: p!n!phosphate 5ishma and .erner ,'4trate: diazoni'm phenyl phosphate End prod'ct: phenol 6in+ Armstron+ ,'4trate: diazoni'm phenyl phosphate End prod'ct: phenol and Phosphate 0essy7 .o1ry and 0rac8 ,'4trate: P9PP End prod'ct: p!n!phenol Citrate 4'--er (pH 4.()

0a4son7 #ead7 Philips ,'4strate: alpha!naphthyl phosphate Citrate 4'--er (pH ".$) 2eas're: alpha!naphthol release 1hen added 1: &!dianisidine #oy et al 2ethod ,'4strate: ,odi'm *2P Color de/eloper: 9a&H!9a2C&3 sol'tion ,er'm preser/ati/e: Acetate 0'---er (pH") End prod'ct: -ree *hymolphthalein End color: 0l'e Practical concideration: ,er'm ACP sli+htly hi+her than plasma apparently d'e to the release o- AC5 -rom platelets d'rin+ clottin+. *he 'se o- *2P as s'4strate has pro/en to 4e the most speci-ic s'4strate -or ACP. #e;ected i- there si+ni-icant amo'nt #CP Chylo's sera analytical di--ic'lty ,er'm sho'ld separate in #0C

Clinical ,i+ni-icance *otal ACP (moderately ele/ated) 5emale 4reast cancer Pa+et<s disease Hyperparathyroidism

9on!prostatic ACP is ele/ated #e-erence #an+e *otal ACP male: 2."!11.> I?:. -emale: %.3!(.2 I?:. 3a'cher<s disease 9eimann!Pic8 =isease Hematolo+ical disorder (2yelocytic .e'8emia)

Prostatic ACP 2ale: %.2!3."I?:. 5emale: %.%!%. I?:l