Physical & Chemical Means ofControlling Contamination inPlant Tissue Culture
Dr. Gary SeckingerDr. Kenneth C. Torres
Phyto
Technology Laboratories
24 May 20042004 WORLD CONGRESS on In Vitro Biology
“Like death and taxes, contamination or thethreat of contamination is always with us andwe need all the weapons to combat them.”
L.L. Coriell National Cancer Institute Monog. 7:33, 1962
24 May 20042004 WORLD CONGRESS on In Vitro Biology
Physical Means of ControllingContamination
Eliminate vector transmission of contaminants:
•Seal Culture Vessels –Parafilm, Nescofilm –PVC Film (Austratec)•Seal Cultures in Plastic Bags: –e.g., Zip-lock Bags,Star*Pac Bags
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