Plant antibiotics production through tissue culture

• Antibiotics:
• Antibiotics are substances produced by certain microorganisms that suppress the growth of other microorganisms and eventually destroy them.
• PREPARATION OF PLANT TISSUE CULTURE MEDIUM
• Measure approximately 90% of the required volume of the deionized-distilled water in a flask/container of double the size of the required volume.
• Add the dehydrated medium into the water and stir to dissolve the medium completely. Gentle heating of the solution may be required to bring powder into solution
• Add desired heat stable supplements to the medium solution.
• Add additional deionized-distilled water to the medium solution to obtain the final required volume.
• Set the desired pH with NaOH or HCl.
• Dispense the medium into culture vessels
• Sterilize the medium by autoclaving at 15 psi (121°C) for appropriate time period.

Higher temperature may result in poor cell growth.

•Add heat labile supplements after autoclaving.

Examples of some antibiotics

• 4-Bromo-antibiotic A23187
• Alamethicin system Amikacin Antibiotic, Amoxicillin Antibiotic, Amphotericin B
• Antibiotic, Ampicillin Antibiotic, Anisomycin Antibiotic, Antimycotics