As most microorganisms are potentially pathogenic it is essential that they be handled by procedures which avoid contamination of the microbiologist. Furthermore, since microorganismsare so ubiquitous it is necessary to avoid contamination of microbial culture by stray microorganisms.
–To become familiar with the basic movements of aseptic culture technique and our owntechnique.
1) Inoculums transfer(A)Technique of transferring culture from one agar plate to another bystreakingMaterials
1 wire loopPlate culture of
1 nutrient agar plateBunsen burner
ProcedureRemember that the agar is a fragile medium and only gentile pressure with the oop isrequired.
1.The Bunsen burner was lighted and the wire loop was holed by right hand.
The wire loop is then sterilized by flaming it in the Bunsen burner until it is red heat. Thewire loop was allowed for 5 seconds to cool it down.
The lid of Petri dish containing
was opened by using left hand. Then, small loopwas removed from the inoculums and the lid was closed immediately.
The second Petri dish was opened with left hand and the inoculum was spread at the smallarea at the top edge of the agar and spread it over a small area (primary inoculums). Thenthe loop was flamed.5.After the loop was cooled make four or five single strokes starting from the primaryinoculums. The loop was flamed again and overlapping series of stroke covering anther area was made until all the surface of the agar was covered.
The plate was closed and was placed inverted for incubation at 37’C for 24-48 hours.
(A)Technique of transferring culture from an agar plate to a bottle of nutrientbroth