Freezing Point Depression Method
CALCULATION FOR SOLUTION ISOTONIC WITH BLOOD AND TEARS
Some of the many ways;1.
Freezing point depression method2.
NaCl equivalent method3.
White Vincent method4.
Molecules concentration method6.
Graphical method on vapor pressure and freezing point determination
FREEZING POINT DEPRESSION METHOD
The plasma and blood freezing point temperature = -0.52°C
The dissolved substances in plasma or tear depress the solution freezing point below 0.52°C
Any solution that freeze at T=-0.52°C is isotonic with blood and tear
weight of substance that need to be adjusted to make it hypotonic given by the followingformula;
Where,W=the weight, in g, of the added substance in 100ml of the final solutiona =the depression of the freezing point of water produced by the medicament already in thesolution (calculated by multiplying the value for b for the medicament by the strength of thesolution express as % w/v)b=the depression of freezing of water produced by 1 per cent w/v of the added substance