Description

Apoptosis
Methods in
Pharmacology
and Toxicology
Approaches to
Measurement
and Quantitation
Edited by
Myrtle A. Davis

1 Introduction
Myrtle A. Davis ......................... ......................... ......................... ...... 1
2 Flow Cytometric and Fluorometric Methods of Quantifying
and Characterizing Apoptotic Cell Death
Martin Poot, Robert H. Pierce, and Terrance J. Kavanagh..... 11
3 Analysis of Apoptosis by Laser-Scanning Cytometry
Zbigniew Darzynkiewicz, Elzbieta Bedner,
and Piotr Smolewski ......................... ......................... ................ 37
4 Specific Methods for Detection and Quantification
of Apoptosis in Tissue Sections
Matthew A. Wallig, Curtis M. Chan, and Nancy A. Gillett ..... 59
5 DNA Microarrays: An Overview of Technologies
and Applications
Helmut Zarbl ......................... ......................... ......................... ......... 77
6 Microarray Analysis of Apoptosis
Richard W. E. Clarkson, Catherine A. Boucher,
and Christine J. Watson ......................... ......................... .......... 97
7 ELISAs for Quantification of Bcl-2 Family Activities
and Active Caspases
Calvin F. Roff, Amy M. Walz, Lisa B. Niehoff,
David J. Sdano, Antoinette M. Bennaars, Jeffrey A. Cooper,
Becky L. Senft, Anatoli A. Sorkin, Steven P. Stoesz,
and Paul A. Saunders ......................... ......................... ............ 119
Index ......................... ......................... ......................... ..................... 149
Contents
xi

xii Contributors
Contributors
ELZBIETA BEDNER • Department of Pathology, Pomeranian School of
Medicine, Szczecin, Poland
ANTOINETTE M. BENNAARS • R & D Systems Inc. Minneapolis, MN
CATHERINE A. BOUCHER • Biorobotics Ltd., Cambridge, UK
CURTIS M. CHAN • Sierra Biomedical, A Division of Charles River
Laboratories, Inc., Sparks, NV
RICHARD W. E. CLARKSON • Department of Pathology, University of
Cambridge, Cambridge, UK
JEFFREY A. COOPER • R & D Systems Inc., Minneapolis, MN
ZBIGNIEW DARZYNKIEWICZ • Brander Cancer Research Institute, New York
Medical College, Valhalla, NY
MYRTLE A. DAVIS • Department of Pathology, University of Maryland
School of Medicine, Baltimore, MD
NANCY A. GILLETT • Sierra Biomedical, A Division of Charles River
Laboratories, Inc., Sparks, NV
MANNFRED A. HOLLINGER • Emeritus Professor of Pharmacology, School
of Medicine, University of California, Davis, CA
TERRANCE J. KAVANAGH • Department of Environmental Health, University
of Washington, Seattle, WA
LISA B. NIEHOFF • R & D Systems Inc., Minneapolis, MN
ROBERT H. PIERCE • Department of Pathology, University of Rochester,
Rochester, NY
MARTIN POOT • Department of Pathology, University of Washington,
Seattle, WA
CALVIN F. ROFF • R & D Systems Inc., Minneapolis, MN
PAUL A. SAUNDERS • R & D Systems Inc., Minneapolis, MN
DAVID J. SDANO • R & D Systems Inc., Minneapolis, MN
BECKY L. SENFT • R & D Systems Inc., Minneapolis, MN
PIOTR SMOLEWSKI • Department of Hematology, School of Medicine,
Lodz, Poland
ANATOLI A. SORKIN • R & D Systems Inc., Minneapolis, MN
STEVEN P. STOESZ • R & D Systems Inc., Minneapolis, MN
xiii
Contributors xiv
MATTHEW A. WALLIG • Department of Veterinary Pathobiology, University
of Illinois at Urbana-Champaign, Urbana, IL
AMY M. WALZ • R & D Systems Inc., Minneapolis, MN
CHRISTINE J. WATSON • Department of Pathology, University of Cambridge,
Cambridge, UK
HELMUT ZARBL • Cancer Biology Program, Fred Hutchinson Cancer
Research Center, Seattle, WA