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Koronavirus adalah virus dari famili Coronaviridae yang dapat menyebabkan

penyakit pada manusia, mamalia, dan burung. Struktur tubuh virus (virion) ini
terdiri dari membran, selubung lipid bilayer (envelope), glikoprotein yang
menyerupai paku (spike), genom RNA positif, dan protein nukleokapsid.
Glikoprotein koronovirus dapat berikatan dengan glikoprotein permukaan sel
inang secara spesifik untuk memulai terjadinya infeksi.

Koronavirus

diklasifikasikan menjadi tiga golongan utama, golongan 1 dan 2 menginfeksi

mamalia, mulai dari kelelawar hingga manusia, sedangkan golongan 3 hanya
ditemukan pada spesies avian (burung). Infeksi virus ini dapat menimbulkan
gejala penyakit yang bervariasi, mulai dari hampir tidak timbul gejala apapun
hingga gejala yang fatal dan cepat.

Infeksi koronavirus dapat menyebabkan

berbagai penyakit, seperti bronkitis, ensefalitis, gastroenteritis, dan hepatitis

(Wikipedia, 2013)
Coronavirus adalah virus yang berbentuk bulat dan berdiameter sekitar 100-120
nm. Karena itu, pencegahan infeksi Coronavirus akan efektif bila menggunakan
masker yang berpori-pori lebih kecil dari 100 nm. Virus ini memiliki RNA
positive sebagai genomnya, dan biasanya sering disebut virus RNA. Mutasi virus
terjadi pada saat replikasi dan virus RNA bermutasi sekitar 1 juta kali lebih cepat
dari pada virus DNA. Kalau virus DNA mempunyai kecepatan mutasi 10-8
sampai 10-11 nukleotida setiap kali proses replikasi, virus RNA berkecapatan 103 sampai 10-4. Karena itu, tidak bisa dimungkiri bahwa virus penyebab SARS
adalah Coronavirus yang sudah bermutasi (Berita iptek. 2010)

Panjang genom Coronavirus berkisar antara 27 sampai 32 kilobasa. Genom ini

membentuk protein-protein pembentuk tubuh virus seperti fosfoprotein N,
glikoprotein M, protein E, protein S, dan glikoprotein HE, dan prtotein-protein
atau enzim-enzim yang perlu untuk replikasi virus itu sendiri.

Prosedur Penelitian
1.

Pencarian Data Protein

Dicari sekuen protein dari spike glycoprotein human corona virus 229E melalui
National

Centre

of

Biotechnology

Information

(NCBI)

(http://www.ncbi.nlm.nih.gov/). Data yang diperoleh kemudian disimpan dalam

bentuk FASTA pada Notepad untuk kemudian digunakan pada seleksi data.
2.

Pencarian kristal struktur

Dicari kristal struktur spike glycoprotein human corona virus 229E dan reseptor
spesifik human corona virus 229E yaitu APN (Aminopeptidase N) melalui Protein
Data Bank (PDB) (http://www.rcsb.org/pdb/home/home.do)
3.

Seleksi Data dengan Alignment (Pensejajaran)

Alignment protein dilakukan dengan menggunakan softwere MEGA 5.05 (metode

Clustal W).
4.

Pembuatan pohon filogeni

Pohon filogeni di buat menggunakan softwere MEGA 5.05 (metode Clustal W).
5.

Modelling protein

Modelling protein spike glycoprotein Human Corona Virus 229E dilakukan

melalui SWISS MODEL (http://swissmodel.expasy.org/) dengan menggunakan
template dari kristal struktur spike glycoprotein Human Corona Virus NL63 dari
PDB dengan kode akses 2IEQ
6.

Analisis Docking

Analisis Docking dilakukan melalui cluspro (http://cluspro.bu.edu/login.php)

HASIL DAN PEMBAHASAN

Sequen protein spike glicoprotein Corona Virus 229E

Squens 1

>gi|82780501|gb|ABB90530.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGACKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNETFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQI

Squens 2

>gi|82780499|gb|ABB90529.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGACKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFF
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI

QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 3

>gi|82780497|gb|ABB90528.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGACKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVESQSKRYGFGGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ

Squens 4

>gi|82780495|gb|ABB90527.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGYSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGACKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNETFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 5

>gi|82780493|gb|ABB90526.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL

ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIHDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 6

>gi|82780487|gb|ABB90523.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ

TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSERYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 7

>gi|82780485|gb|ABB90522.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFDVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNETFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ

Squens 8

>gi|82780483|gb|ABB90521.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSQSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTFQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 9

>gi|82780481|gb|ABB90520.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSQSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHNIGSLYVSWSDGD

VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTFQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 10

>gi|82780479|gb|ABB90519.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFKLRSGVGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GAKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVSTALNKIQDVVNQQGNS

LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 11

>gi|82780477|gb|ABB90518.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFKLRSGVGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GAKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ

Squens 12

>gi|82780473|gb|ABB90516.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSD

FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFKLRSGVGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GAKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 13

>gi|82780471|gb|ABB90515.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FERNLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKLQSGVGRCFNCRPAVVNITLANFN
ETKGPLCVDTSHFTTKFV
GANFGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANLA
YLNSYTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST

SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSIVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 14

>gi|82780469|gb|ABB90514.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTFAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKLQSGVGRCFNCRPAVVNITLANFN
ETKGPLCVDTSHFTTKFV
GANFGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANLA
YLNSYTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT

LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTVP
DLVVEQYNQIILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWLN
RVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 15

>gi|82780467|gb|ABB90513.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFSSNVSSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSSFVGALPKTVRE
FVIARTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGKCYNCRPAVVNITLANFN
ETKGPLCVETSHFTTKYV
AANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANL
AYINSYTIGSLYVSWSDGD
GITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGEYNLSSVVPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRVPTMADFVQIENCNVTFVNISRSELQTIVPEYIDV
NKTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ

Squens 16

>gi|82780465|gb|ABB90512.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV

VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFSSNVSSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSSFVGALPKTVRE
FVIARTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGRCYNCRPAVVNITLANFN
ETKGPLCVETSHFTTKYV
AANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANL
AYINSYTIGSLYVSWSDGD
GITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGEYNLSSVVPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRVPTMADFVQIENCNVTFVNISRSELQTIVPEYIDV
NKTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 17

>gi|82780463|gb|ABB90511.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKLQSGVGRCFNCRPAVVNITLANFN
ETKGPLCVDTSHFTTKYV
GANFGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANLA
YLNSYTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS

ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSIVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 18

>gi|82780461|gb|ABB90510.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKLQSGVGRCFNCRPAVVNITLANFN
ETKGPLCVDTSHFTTKYV
GANFGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANLA
YLNSYTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK

VNECVKSQSKRYGFCGNGTHIFSIVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 19

>gi|82780459|gb|ABB90509.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIADCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFSSNVSSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSSFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGKCYNCRPAVVNITLANFN
ETKGPLCVETSHFTTKYV
AANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANL
AYINSYTIGSLYVSWSDGD
GITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAIVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCTD
AVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ

Squens 20

>gi|82780457|gb|ABB90508.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFSSNVSSDVIRYNIN

FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSSFVGALPKTVRE
FVIARTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGRCYNCRPAVVNITLANFN
ETKGPLCVETSHFTTKYV
AANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANL
AYINSYTIGSLYVSWSDGD
GITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGEYNLSSVVPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRVPTMADFVQIENCNVTFVNISRSELQTIVPEYIDV
NKTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 21

>gi|82780455|gb|ABB90507.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSHSVCNGCVGYSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFSSNVSSDVIRYNIN
FEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSSFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGKCYNCRPAVVNITLANFN
ETKGPLCVETSHFTTKYV
AANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANL
AYINSYTIGSLYVSWSDGD
GITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCT

DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 22

>gi|82780491|gb|ABB90525.1| spike glycoprotein [Human coronavirus 229E]

VDCSTYVCNGNVRCVELLKQYTSACKTIEDALRNSAMLESADVSEMLT
FDKKAFTLANVSSFGDYNLSSV
IPSLPRSGSRVAGRSAIEDILFSKLVTSGLGTVDADYKKCTKGLSIADLA
CAQYYNGIMVLPGVADAERM
AMYTGSLIGGIALGGLTSAASIPFSLAIQSRLNYVALQTDVLQENQKILA
ASFNKAMTNIVDAFTGVNDA
ITQTSQALQTVATALNKIQDVVNQQGNSLNHLTSQLRQNFQAISSSIQAI
YDRLDIIQADQQVDRLITGR
LAALNVFVSHTLTKYTEVRASRQLAQQKVNECVKSQSKRYGFCGNGT
HIFSLVNAAPEGLVFLHTVLLPT
QYKDVEAWSGLCVDGRNGYVLRQPNLALYKEGNYYRITSRIMFEPRIP
TIADFVQIENCNVTFVNISRSE
LQTIVPEYIDVNKTLQELSYKLPNYTVPDLVVEQYNQTILNLTSEISTLE
NKSAELNYTVQKLQTLIDNI
NSTLVDLKWLNRVETYIKWPWWVWLCISVVLIFVVSMLLLCCCSTGCC
GFFSCFASSIRGCCESTKLPYY
DVEKIHIQ

Squens 23

>gi|82780489|gb|ABB90524.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRLTTGFVYFNGTGRGACKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI

QSVELPVSIVSLPVYHKHTFIVLYVNFKLRRGPGRCYNC
>gi|82780475|gb|ABB90517.1| spike glycoprotein [Human coronavirus 229E]
MFVLLVAYALLHIAGCQTTNGMNTSHSVCNGCVGHSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTNAETTVCTVALASYADVLVNVSQTAIANIIYCNSVINRLR
CDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFKLRSGVGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GAKFDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANL
ANLNSHTIGSLYVSWSDGD
VITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTST
SGNLLGFKDVTNGTIYS
ITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNCT
DAVLTYSSFGVCADGSIIA
VQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNG
NVRCVELLKQYTSACKTI
EDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVTS
GLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLI
GGIALGGLTSAASIPFSLAI
QSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQ
TVATALNKIQDVVNQQGNS
LNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSHT
LTKYTEVRASRQLAQQK
VNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAWS
GLCVDGRNGYVLRQPNLAL
YKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVNK
TLQELSYKLPNYTVP
DLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCIS
VVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 24

>gi|82780453|gb|ABB90506.1| spike glycoprotein [Human coronavirus 229E]

MFVLLVAYALLHIAGCQTTNGLNTSYSVCNGCVGYSENVFAVESGGYIPSD
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGLRFTTGFVYFNGTGRGDCKGFSSDVLSDVIRYNL
NFEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFT
LGNVEAVNFNVTTAETTDFCTVALASYADVLVNVSQTSIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSP
IQSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGKCFNCYPAGVNITLANFN

ETKGPLCVDTSHFTTKY
VAVYANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIV
ANWAYSKYYTIGSLYVSWS
DGDGITGVPQPVEGVSSFMNVTLDKCTKYNIYDVSGVGVIRVSNDTFLNGIT
YTSTSGNLLGFKDVTKGT
IYSITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYN
CTDAVLTYSSFGVCADGS
IIAVQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCN
GNVRCVELLKQYTSAC
KTIEDALRNSARLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSG
SRVAGRSAIEDILFSKL
VTSGLGTVDADYKNCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYT
GSLIGGIALGGLTSAVSIPFS
LAIQARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQ
ALQTVATALNKIQDVVNQQ
GNSLNHLTSQLRQNFQAISSSIQAIYDRLDTIQADQQVDRLITGRLAALNVFV
SHTLTKYTEVRASRQLA
QQKVNECVKSQSKRYGFCGNGTHIFSIVNAAPEGLVFLHTVLLPTQYKDVE
AWSGLCVDGTNGYVLRQPN
LALYKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYI
DVNKTLQELSYKLPNY
TVPDLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLK
WLNRVETYIKWPWWVWL
CISVVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ
Squens 25

>gi|1906323|emb|CAA71147.1| anti-receptor protein [Human coronavirus 229E]

SGTGRGDCKGFSSDVLSDVIRYILNFEENLRRGTILFKTSYGVVVFYCTNNT
LVSGDAHIPFGTVLGNFY
CFVNTTIGNETTSAFVGALPKTVREFVISRTGHFYINGYRYFTLGNVEAVNF
NVTTAETTDFLTVALASY
ADVLVNVSQTSIANIIYCNSVINRLRCDQLSFDVPDGFYSTSPIQSVELPVSIV
SLPAYHKHTFIVLYVD
FKPQSGGGKCFNCYPAGVNITLANFNETNGPLCVDTSHFTTKYVAVYANVG
RWSASINTGNCPFSFGKVN
NFVKFGSVCFSLKDIPGGCAMPIVANWAYSKYYTIGSLYVSWSDGDGITGV
PQPVEGVSSFMNATLDKCT
KYNIYDVSGVGVIRVSNDTFLNGITYTSTSGNLLGFKDVTKGTIYSITPCNPP
DQLVVYQQAVVGAMLSE
NFTSYGFSNVVELPKFFYASNGTYNCTDAVLTYSSFGVCADGSIIAVQPRNV
SYDSVSAIVTANLSIPSN
WTTSVQVEYLQITSTPIVVDCSTYVCNGNVRCVELLKQYTSACKTIEDALRN
SARLESADVSEMLTFDKK
AFTLANVSSFGDYNLSSVIPSLPTSGSRVAGRSAIEDILFSKLVTSGLGTVDA
NYKKCTKGLSIADLACA
QYYNGIMVLPGVADAERMAMYTGSLIGGIALGGLTSAVSIPFSLAIQARLNY
VALQTDVLQENQKILAAS
FNKAMTNIVDAFTGVNDAITQTSQALQTVATALNKIQDVVNQQGNSLNHLT

SQLRQNFQAISSSIQAIYD
RLDTIQADQQVDRLITGRLAALNVFVSHTLTKYTEVRASRQLAQQKVNECV
KSQSKRYGFCGNGTHIFSI
VNAAPEGLVFLHTVLLPTQYKDVEAWSGLCVDGTNGYVLRQPNLALYKEG
NYYRITSRIMFEPRIPTMAD
FVQIENCNVTFVNISRSELQTIVPEYIDVNKTLQELSYKLPNYTVPDLVVEQY
NQTILNLTSEISTLENK
SAELNYTVQKLQTLIDNINSTLVDLKWLNRVETYIK
Squens 26

>gi|1906322|emb|CAA71146.1| anti-receptor protein [Human coronavirus 229E]

NGTGRGDCKGFSSDVLSDVIRYNLNFEENISRGTILFKTSYGVVVFYCTNNT
LVSGDAHIPFGTVLGNFY
CFVNTTIGNETTSAFVGALPKTVREFVISRTGHFYINGYRYFSLGNVEAVNF
NVTNAETTVCTVALASYA
DVLVNVSQTAIANIIYCNSVINRLRCDQLSFDVPDGFYSTSPIQSVELPVSIVS
LPVYHKHTFIVLYVNF
KLRSGVGRCYNCRPAVVNITLANFNETKGPLCVDTSHFTTQFVGAKFDRWS
ASINTGNCPFSFGKVNNFV
KFGSVCFSLKDIPGGCAMPIMANLANLNSHNIGSLYVSWSDGDVITGVPKPV
EGVSSFMNVTLNKCTKYN
IYDVSGVGVIRISNDTFLNGITYTSTSGNLVGFKDVTNGTIYSITPCNPPDQLV
VYQQAVVGAMLSENFT
SYGFSNVVEMPKFFYASNGTYNCTDAVLTYSSFGVCADGSIIAVQPRNVSY
DSVSAIVTANLSIPSNWTT
SVQVEYLQITSTPIVVDCSTYVCNGNVRCVELLKQYTSACKTIEDALRNSAM
LESADVSEMLTFDKKAFT
LANVSSFGDYNLSSVIPSLPRSGSRVAGRSAIEDILFSKLVTSGLGTVDADYK
KCTKGLSIADLACAQYY
NGIMVLPGVADAERMAMYTGSLIGGIALGGLTSAASIPFSLAIQSRLNYVAL
QTDVLQENQKILAASFNK
AMTNIVDAFTGVNDAITQTSQALQTVATALNKIQDVVNQQGNSLNHLTSQL
RQNFQAISSSIQAIYDRLD
IIQADQQVDRLITGRLAALNVFVSHTLTKYTEVRASRQLAQQKVNECVKSQ
SKRYGFCGNGTHIFSLVNA
APEGLVFLHTVLLPTQYKDVEAWSGLCVDGRNGYVLRQPNLALYKEGNYY
RITSRIMFEPRIPTIADFVQ
IENCNVTFVNISRSELQTIVPEYIDVNKTLQELSYKLPNYTVPDLVVEQYNQT
ILNLTSEISTLENKSAE
LNYTVQKLQTLIDNINSTLVDLKWLNRVETYIK

Squens 27

>gi|1869755|emb|CAA71056.1| spike protein [Human coronavirus 229E]

NGTGRGDCKGFSSDVLSDVIRYNLNFEENLRRGTILFKTSYGVVVFYCTNNT
LVSGDAHIPFGTVLGNFY
CFVNTTIGNETTSAFVGALPKTVREFVISRTGHFYINGYRYFTLGNVEAVNF
NVTTAETTDFFTVALASY
ADVLVNVSQTSIANIIYCNSVINRLRCDQLSFDVPDGFYSTSPIQSVELPVSIV
SLPVYHKHTFIVLYVD

FKPQSGGGKCFNCYPAGVNITLANFNETKGPLCVDTSHFTTKYVAVYANVG
RWSASINTGNCPFSFGKVN
NFVKFGSVCFSLKDIPGGCAMPIVANWAYSKYYTIGSLYVSWSDGDGITGV
PQPVEGVSSFMNVTLDKCT
KYNIYDVSGVGVIRVSNDTFLNGITYTSTSGNLLGFKDVTKGTIYSITPCNPP
DQLVVYQQAVVGAMLSE
NFTSYGFSNVVELPKFFYASNGTYNCTDAVLTYSSFGVCADGSIIAVQPRNV
SYDSVSAIVTANLSIPSN
WTTSVQVEYLQITSTPIVVDCSTYVCNGNVRCVELLKQYTSACKTIEDALRN
SARLESADVSEMLTFDKK
AFTLANVSSFGDYNLSSVIPSLPTSGSRVAGRSAIEDILFSKLVTSGLGTVDA
DYKKCTKGLSIADLACA
QYYNGIMVLPGVADAERMAMYTGSLIGGIALGGLTSAVSIPFSLAIQARLNY
VALQTDVLQENQKILAAS
FNKAMTNIVDAFTGVNDAITQTSQALQTVATALNKIQDVVNQQGNSLNHLT
SQLRQNFQAISSSIQAIYD
RLDTIQADQQVDRLITGRLAALNVFVSHTLTKYTEVRASRQLAQQKVNECV
KSQSKRYGFCGNGTHIFSI
VNAAPEGLVFLHTVLLPTQYKDVEAWSGLCVDGTNGYVLRQPNLALYKEG
NYYRITSRIMFEPRIPTMAD
FVQIENCNVTFVNISRSELQTIVPEYIDVNKTLQELSYKLPNYTVPDLVVEQY
NQTILNLTSEISTLENK
SAELNYTVQKLQTLIDNINSTLVDLKWLNRVETYIK

1. ALLIGNMENT
2. SELEKSI
3. MODELLING

Hasil Homology Modelling

Sequence
>gi|82780491|gb|ABB90525.1| spike glycoprotein [Human coronavirus 229E]
VDCSTYVCNGNVRCVELLKQYTSACKTIEDALRNSAMLESADVSEMLTFD
KKAFTLANVSSFGDYNLSSV
IPSLPRSGSRVAGRSAIEDILFSKLVTSGLGTVDADYKKCTKGLSIADLACAQ
YYNGIMVLPGVADAERM
AMYTGSLIGGIALGGLTSAASIPFSLAIQSRLNYVALQTDVLQENQKILAASF
NKAMTNIVDAFTGVNDA
ITQTSQALQTVATALNKIQDVVNQQGNSLNHLTSQLRQNFQAISSSIQAIYDR
LDIIQADQQVDRLITGR
LAALNVFVSHTLTKYTEVRASRQLAQQKVNECVKSQSKRYGFCGNGTHIFS
LVNAAPEGLVFLHTVLLPT
QYKDVEAWSGLCVDGRNGYVLRQPNLALYKEGNYYRITSRIMFEPRIPTIA
DFVQIENCNVTFVNISRSE
LQTIVPEYIDVNKTLQELSYKLPNYTVPDLVVEQYNQTILNLTSEISTLENKS
AELNYTVQKLQTLIDNI
NSTLVDLKWLNRVETYIKWPWWVWLCISVVLIFVVSMLLLCCCSTGCCGFF
SCFASSIRGCCESTKLPYY
DVEKIHIQ

Hasil modelling

Sequence no. 1
(model 1)

>gi|300834741|gb|ADK37701.1| spike glycoprotein [Human coronavirus 229E]

TNGMNTSYPVCNGRVGHSGNVFAVESGGYIPSNFAFNNWFLLTNTSSVVDG
VVRSFQPLLLNCLWSVSGS
RFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNINFEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIP
SGTVLGNFYCFVNTTIDNETTSAFVGALPKTVREFVISRTGHFYINGYRYFSL
GNVEAVNFNVTNAATTV

Sequence no. 10

CTVALASYADVLVNVSQTAIANIIYCNSVINRLRCDQLSFDVPDGFYSTSPIQ
SVELPVSIVSLPVYHKH

(model 3)

TFIVLYVNFELRRGPGRCYNCRPAVVNITLANFNETKGPLCVDTSHFTTQFV
GVKFDRWSASINTGNCPF
SFGKVNNFVKFGSVCFSLKDIPGGCAMPIMANLVNHKSHNIGSLYVSWSDG
DVITGVPKPVEGVSSFMNV
TLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTSTSGNLLGFKDVTNGTIYSIT
PCNPPDQLVVYQQAVA
GAMLSENFTSYGFSNVVEMPKFFYASNGTYNCTDAVLTYSSFGVCADGSIIA
VQPR
>gi|385215157|gb|AFI49431.1| spike glycoprotein [Alpaca respiratory coronavirus]
MFVLVAYALLHIAGCQTINVLNTSHSVCNGCVGYSENVFAVESGGYIPSDF
AFNNWFLLTNTSSLVDGVV
RSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFSSNVLSDVIRYNINF
EENLRRGTILFKTSFGVV
VFYCTNNTLVSGDAYIPFGTVLGNFYCFVNTTIGNETTSSFVGALPKTVREF
VISRTGHFYINGYRYFTL
GNVEAVNFNVTNAETTFCTVALASYADVLVNVSQTAIANIIYCNSVINRLRC
DQLSFDVPDGFYSTSPIQ
SVELPVSIVSLPVYHKHTFIVLYVDFKPQSGAGTCYNCRPSVVNITLANFNET
KGPLCVETSHFTTKYVA
SNVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANFAY
INSYTIGSLYVSWSDGDG
ITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTSTS
GNLLGFKDVTNGIIYSI
TPCNPPDQLVVYQQAVVGAMLSENSSSYGFSNVVELPNFFYASNGTYNCTD
AVLTYSSFGVCADGSIIAV
QPRNVSYDGVSAIVTANLSIPSNWTTSVQVEYLQITSTPILVDCSTYVCNGN
VRCVELLKQYTSACKTIE
DALRISAMLESADVGEMLTFDEKAFTLANVSSFGDYNLSSVIPSLPTSGSRV
AGRSAIEDILFSKVVTSG
LGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLIG
GIALGGLTSAAAIPFSLALQ
ARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQAIQT
VATALNKIQDVVNQQGNAL
NHLTSQLRQNFQAISSSIQAIYDRLDTIQADQQVDRLITGRLAALNAFVAQTL
TKYTEVRASRQLAQQKV
NECVKSQSNRYGFCGNGTHIFSIVNSAPEGLVFLHTVLLPTQYKDVEAWSGL
CVDNTNGYVLRQPNLALY

Sequence no. 11
(model 4)

KEGNYYRITSRFMFEPRIPTMADFVQIEKCNVTFVNISRSELQTIVPEYIDVNK
TLQELIDKLPNYTVPD
LGIDQYNQTILNLTSEISTLENKSAELNYTVQRLQTLIDNINSTLVDLKWLNR
VETYLKWPWWVWLCISV
VLIFVVSILLLCCCSTGCCGFFSCLASSTRGCCESTKLPYYDVEKIHIQ
>gi|404553532|gb|AFR79250.1| spike protein [Human coronavirus 229E]
MFVLLVAYALLHIAGCQTTNGTNTSHSVCNGCVGHSENVFAVESGGYIPSN
FAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGSRFTTGFVYFNGTGRGDCKGFYSNASSDVIRYNIN
FEENLRRGTILFKTSYGA
VVFYCTNNTLVSGDAHIPSGTVLGNFYCFVNTTIDNETTSAFVGALPKTVRE
FVISRTGHFYINGYRYFS
LGNVEAVNFNVTNAATTVCTVALASYADVLVNVSQTAIANIIYCNSVINRL
RCDQLSFDVPDGFYSTSPI
QSVELPVSIVSLPVYHKHTFIVLYVNFELRRGPGRCYNCRPAVVNITLANFN
ETKGPLCVDTSHFTTQFV
GNVKLDRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIMAN
LVNHKSHNIGSLYVSWSDG
DVITGVPKPVEGVSSFMNVTLNKCTKYNIYDVSGVGVIRISNDTFLNGITYTS
TSGNLLGFKDVTNGTIY
SITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVEMPKFFYASNGTYNC
TDAVLTYSSFGVCADGSII
AVQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIXVDCSTYVCNG
NVRCVELLKQYTSACKT
IEDALRNSAMLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPRSGSR
VAGRSAIEDILFSKLVT
SGLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGS
LIGGIALGGLTSAASIPFSLA
IQSRLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQAL
QTVATALNKIQDVVNQQGN
SLNHLTSQLRQNFQAISSSIQAIYDRLDIIQADQQVDRLITGRLAALNVFVSH
TLTKYTEVRASRQLAQQ
KVNECVKSQSKRYGFCGNGTHIFSLVNAAPEGLVFLHTVLLPTQYKDVEAW
SGLCVDGINGYVLRQPNLA
LYKEGNYYRITSRIMFEPRIPTIADFVQIENCNVTFVNISRSELQTIVPEYIDVN
KTLQELSYKLPNYTV
PDLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWL
NRVETYIKWPWWVWLCI
SVVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ

Sequence no. 12
(model 2)

>gi|37222542|gb|AAQ90011.1| spike glycoprotein

[Human coronavirus 229E]
MFVLLVAYALLHIAGCQTTNGLNTSYSVCNGCVGY
SENVFAVESGGYIPSDFAFNNWFLLTNTSSVVDGV
VRSFQPLLLNCLWSVSGLRFTTGFVYFNGTGRGD
CKGFSSDVLSDVIRYNLNFEENLRRGTILFKTSYGV
VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGN
ETTSAFVGALPKTVREFVISRTGHFYINGYRYFT
LGNVEAVNFNVTTAETTDFFTVALASYADVLVNVS
QTSIANIIYCNSVINRLRCDQLSFDVPDGFYSTSP
IQSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGK
CFNCYPAGVNITLANFNETKGPLCVDTSHFTTKY
VAVYANVGRWSASINTGNCPFSFGKVNNFVKFGS
VCFSLKDIPGGCAMPIVANWAYSKYYTIGSLYVSW
S
DGDGITGVPQPVEGVSSFMNVTLDKCTKYNIYDVS
GVGVIRVSNDTFLNGITYTSTSGNLLGFKDVTKGT
IYSITPCNPPDQLVVYQQAVVGAMLSENFTSYGFS
NVVELPKFFYASNGTYNCTDAVLTYSSFGVCADG
S
IIAVQPR

Error Report Homology Modelling

Hasil modelling yang error ini kemungkinan dipengaruhi oleh

beberapa faktor misalnya :
-

Template kurang specific

Sequence terlalu lemah

Dan sinyal internet yang tidak kontinue

4. DOCKING
1. Docking antara model 1 dengan reseptor CD13 (4fys)
Ribbon

Hasil (model 4)
Surface

Center -630.4
Lowest Energy -630.4
2. Docking antara model 1 dengan reseptor CD13 (4fyr)
Ribbon

Hasil (model 4)
Surface

Center -521.6
Lowest Energy -602.9
3. Docking antara model 2 dengan reseptor CD13 (4fys)

Ribbon

Hasil (model 4)
Surface

Center -630.4
Lowest Energy -630.4
4. Docking antara model 2 dengan reseptor CD13 (4fyr)
Ribbon

Hasil (model 7)
Surface

Center -612.1
Lowest Energy -612.1
5. Docking antara model baru 1 dengan reseptor CD13 (4fys)
Ribbon

Hasil (model 4)
Surface

Center -630.4
Lowest Energy -630.4
6. Docking antara model baru 1 dengan reseptor CD13 (4fyr)
Ribbon

Hasil (model 7)
Surface

Center -612.1
Lowest Energy -612.1
7. Docking antara model baru 2 dengan reseptor CD13 (4fys)
Ribbon

Center -626.6
Lowest Energy -626.6

Hasil (model 6)
Surface

Lowest
Energy

621.4

8. Docking antara mode barul 2 dengan reseptor CD13 (4fyr)

Hasil (model 4)
Surface

Ribbon

Center -612.5
Lowest Energy -612.5

Berdasarkan hasil di atas maka dapat diketahui terdapat interaksi antara

reseptor dan squen yang terlalu lemah