Determination of Biochemical Oxygen Demand (BOD 5)

5-Day BOD Technique Reference: Standard Methods,, Procedure 5210 B BOD na!ysis: "urses and "ures# 1$%&# S'O( Apparatus: 1# )00 *! BOD +ott!es 2# 2 - 5 !iter ,!ass +ott!e -ith si.hon . Avoid using detergents to clean these bottles. Periodically clean with bleach water. )# 20 1" incu+ator &# DO *eter 5# Buret Nutrient Solutions: 1# Phosphate buffer: Disso!/e %#5 , 0(2P0&, 21#15 , 02(P0&, ))#& , 2a2(P0&1(20, and 1#1 , 2(&"! in a..ro3# 500 *! rea,ent -ater# Di!ute to 1 '# The .( shou!d +e 1#2# Store in & " refri,erator# "hec4 +efore each use for conta*ination 5if there is any indication of +io!o,ica!6*icro+ia! ,ro-th, discard re*ainin, rea,ent and .re.are fresh7# 2# agnesium sulfate solution: Disso!/e 22#5 , M,SO&1(20 in rea,ent -ater# Di!ute to 1 '# )# !alcium chloride solution: Disso!/e 21#5 , "a"! 2 in rea,ent -ater# Di!ute to 1 '# &# "erric !hloride solution: Disso!/e 0#25 , 8e"!)9(20 in rea,ent -ater# Di!ute to 1 '# Preparation of Sample: :f any ty.e of ch!orination .rocess is e*.!oyed durin, treat*ent, fina! eff!uent sa*.!es are initia!!y tested for the .resence of residua! ch!orine# Residua! ch!orine can 4i!! the *icroor,anis*s -hich are critica! to *easurin, BOD# 000000001# #he diluted sample used to determine BOD must ha$e a p% bet&een '(5 and )(5 # 8or *unici.a! se-a,e or eff!uent, the .( ran,e is ,enera!!y +et-een 5 - $, +ut the +ufferin, ca.acity of the .hos.hate +uffer -i!! often +rin, the .( of the di!uted sa*.!e +et-een 9#5 and 1#5# 8or un4no-n sa*.!es, chec4 the .( of the di!ution -hich uses the *ost sa*.!e to confir* that the di!utions !ie in the .ro.er .( ran,e# s needed neutra!i;e sa*.!es -ith 12 su!furic acid or 12 sodiu* hydro3ide# Do not di!ute the sa*.!e -ith the acid or +ase +y *ore than 0#5< 51#5 *! in a )00 *! BOD +ott!e7# 2# )# Test fina! eff!uent for residua! ch!orine# Dech!orination is required if a ch!orine residua! is .resent -hen testin, is initiated# See Page BOD - 6# Sa*.!es su.ersaturated -ith disso!/ed o3y,en, o/er a+out %#9 *,6! at 20 " 5and ty.ica! =isconsin a!titudes7, *ay +e encountered durin, -inter *onths or in !oca!ities -here a!,ae are acti/e!y ,ro-in, 5!a,oons7# To .re/ent !oss of o3y,en durin, incu+ation of these sa*.!es, the DO shou!d +e reduced +y sha4in, the sa*.!e or aeratin, it -ith fi!tered co*.ressed air# These types of sa ples often have a high concentration of nitrifying organis s! which can lead to bias in BOD results. "itrification inhibition can be perfor ed! but a per it variance is re#uired if inhibited BODs $%BOD&' are used for re#uired per it reporting # Sa*.!es of untreated industria! -astes, disinfected -astes 5fina! eff!uent7, hi,h te*.erature -astes, or -astes -ith e3tre*e .( /a!ues *ay not contain enou,h *icroor,anis*s to o3idi;e the +iode,rada+!e *atter in the sa*.!es# Such -astes shou!d +e seeded# See BOD Seedin, Procedure on page BOD - ( and )#

&#

Dilution #echni*ue: 1# >sti*ate the B#O#D# of the sa*.!e and se!ect suita+!e di!utions fro* the fo!!o-in, ta+!es: +stimated BOD5 (mg,-) ? 5 ? 10 10 - )0 )0 - 90 90 - $0 Suggested Sample .olumes (m-) 200, 250, )00 100, 150, 200 25, 50, 100 15, 25, 50 10, 15, 25 +stimated BOD5 (mg,-) $0 - 150 150 - )00 )00 - 100 100 - 1500 1500 - 2500 Suggested Sample .olumes (m-) 5, 10, 15 ), 5, 10 1, ), 5 @@@ 0#5, 1, ) @@@ 0#25, 0#5, 1 @@@

BOD - /

Standard *ethods provides additional guidance as follows+ use less than , - for strong industrial wastes! ,-.& - for raw and settled wastewater! and .&-/& - for biologically treated effluent. =hen .re.arin, re.!icate sa*.!es for qua!ity contro! .ur.oses, .re.are the re.!icate at e3act!y the sa*e di!utions as the ori,ina! sa*.!e# See (e!.fu! (ints A2) 5Se.te*+er, 1$$27 for infor*ation re,ardin, the .re.aration and ca!cu!ation of BOD re.!icates# 2# Bsin, a large0tipped, /o!u*etric .i.ette - for sa*.!es !ess than 50 *! - or a ,raduated cy!inder for !ar,er sa*.!e /o!u*es, *easure the .ro.er a*ount of -e!!-*i3ed sa*.!e into thorou,h!y c!eaned and rinsed )00 *! +ott!es# @@@@ Dilutions under 1 m- must be made by diluting the &aste in a graduated cylinder before pipetting # @@@ Di!ution -ater *ay +e .re.ared i**ediate!y +efore use, or, e0cept for the addition of the phosphate buffer , days or -ee4s ahead of ti*e# dd 1 *! or each nutrient so!ution .er !iter of di!ution -ater# The .hos.hate +uffer is the critica! nutrient in sti*u!atin, conta*inatin, ,ro-ths so it *ust +e added the day the -ater is to +e used# Distilled &ater should be allo&ed to e*uilibrate in the incubator or &ith outside air for at least 23 hours at 24 ! before use# To a/oid dust or dirt conta*ination -hi!e a!!o-in, o3y,enation, use a .a.er to-e!, cotton .!u,, or s.on,e to co/er the +ott!e o.enin,# %are is ta1en to ensure that dilution water is o0ygen saturated. The use of aerator stones for this purpose should be discouraged as there is a tendency to develop growths which can prevent dilution water BOD criteria fro being et. The best techni#ue is to use co pressed air which is passed through glass wool or a filter of so e type to prevent the introduction of conta inants # &# >ach BOD +ott!e is fi!!ed +y s!o-!y addin, sufficient di!ution -ater so that the sto..er can +e inserted -ithout !ea/in, an air +u++!e +ut not so *uch that there is o/erf!o-# The si.hon hose *ust +e *ade of sur,ica! ,u* 5!ate3 ru++er7, .o!y.ro.y!ene or .o!yethy!ene to a/oid introducin, BOD into the di!ution -ater# Ty,on and +!ac4 ru++er can add o3y,en de*and to the -ater# 5hen $olumes of sample used exceed /54 ml6 additional nutrients should be added to the sample bottle # dd an additiona! 0#1 *' of nutrients for each 50 *' of sa*.!e used in e3cess of 150 *'# 8or e3a*.!e, if the sa*.!e si;e is 200 *!, an additiona! 0#2 *! is required# 5. "o*.!ete!y fi!! t-o +ott!es -ith di!ution -ater to +e incu+ated as +!an4s# 9# 'a+e! each +ott!e carefu!!y as to sa*.!e and /o!u*e used# R>"ORD O2 B>2"( S(>>T#

)#

7ncubation and Dissol$ed Oxygen (DO) Determinations8 1. "a!i+rate DO *eter each day of use and chec4 *e*+rane on .ro+e# Record the +aro*etric .ressure each day of ana!ysis# This can +e o+tained fro* a +aro*eter in the !a+oratory# !ternati/e!y, +aro*etric .ressure readin,s can +e o+tained /ia the :nternet, fro* a !oca! radio or te!e/ision ne-s station, or fro* a !oca! air.ort# Baro*etric .ressure readin,s shou!d not +e corrected to sea !e/e!# NO#+: as a reference! typical baro etric pressures range fro about 2).6 to ,3., inches $/&..( to /6).6 4 .332.,6 to .326.3/2 illibars' of ercury. Only under severe low pressure syste s $seasonal stor syste s! e.g.! hurricanes or tornadic stor s'! does baro etric pressure drop below 2).3 inches# 2# )# Deter*ine the DO of the t-o di!ution -ater +!an4s and a!! sa*.!e +ott!es and record on data sheet as :nitia! DO# P!ace the sa*.!es and the 2 di!ution -ater +!an4s in a 20 1" incu+ator for 5 days# 8i!! -ater sea!s -ith di!ution -ater and ca. to reduce e/a.oration fro* sea!s# "hec4 dai!y, add -ater to sea!s if necessary# Due to the & day testing period! certain sa ples re#uire that set-ups and run-outs of results be perfor ed by different individuals # Before re*o/in, the ca.s, .our off the -ater a+o/e the ca.# BOD - 2

&#

5#

fter 5 days deter*ine the DO of the t-o di!ution -ater +!an4s and the sa*.!e +ott!es#

!alculations8 :n ,enera!, BOD5 /a!ues !ess than 2#0 *, DO6' shou!d +e re.orted on DMRs as non-detects 5 i#e#, ? 'OD7# Bsin, the data recorded for un-seeded sa*.!es: BOD *,6! C 5:nitia! DO - DO57 3 Di!ution 8actor Di!ution 8actor C Bott!e Do!u*e 5)00 *!7 Sa*.!e Do!u*e
2otes: 1# B!an4 BOD5# :f the DO de.!etion in the di!ution -ater +!an4s e3ceeds 0#2 *,6!, the resu!ts of the test are questiona+!e# The resu!ts of sa*.!es -hich used the sa*e di!ution -ater the Ehi,hE +!an4 shou!d +e qua!ified on the DMR# Do not su+tract +!an4 /a!ues fro* sa*.!e resu!ts# Disso!/ed o3y,en /a!ues of the sa*.!es# On!y di!utions -ith DO de.!etions of at !east 2 *,6', and DO5 of at !east 1 *,6' *ay +e used to ca!cu!ate the sa*.!eFs BOD# /era,e resu!ts fro* sa*.!e di!utions to ca!cu!ate fina! sa*.!e BOD5# See .a,e BOD - $ for ca!cu!ations a..ro.riate for seeded sa*.!es

2# 3. 4.

5f there is no evidence of to0ic effects! average the results fro all dilutions which eet the ini u o0ygen depletion and ini u residual DO re#uire ents. A to0ic effect is indicated when BOD increases significantly as the sa ple dilution increases. This is often referred to as 6sliding BODs7. 8elpful 8ints 9./ $*arch! .)).' provides additional guidance for reporting results.

BOD - 1

Standardi9ation of DO meter 0 5in:ler #itration #echni*ue

Reference: Standard Methods, 1% th edition, Procedure &500-O " ;eagents8 1# 2# )# &# 5# anganous sulfate solution: Disso!/e &%0 , MnS0 &(20 in rea,ent -ater# 8i!terG di!ute to 1 '# Al:ali0iodide0a9ide reagent : Disso!/e 500 , 2aO( and 1)5 , 2a: in rea,ent -ater# Di!ute to 1 '# dd 10 , 2a2) disso!/ed in &0 *! rea,ent -ater# This reagent should not give a color with starch solution when diluted and acidified. !oncentrated Sulfuric acid Standard sodium thiosulfate titrant6 4(4254N8 Purchase co**ercia!!y# Starch Solution: Pre.are an e*u!sion of 5 , so!u+!e starch in a *ortar or +ea4er -ith a s*a!! a*ount of disti!!ed -ater# Pour this e*u!sion into 1 ' of +oi!in, -ater, a!!o- to +oi! a fe- *inutes, and !et sett!e o/erni,ht# Bse the c!ear su.ernate# This so!ution *ay +e .reser/ed +y the addition of 1#25 , sa!icy!ic acid6' and stora,e at & "# /oid addin, at*os.heric

Procedure8 1# S!o-!y si.hon three .ortions of aerated di!ution -ater into three se.arate BOD +ott!es# O2 to di!ution -ater# 2# )# &#

To t-o of the three BOD +ott!es, add 1 *! MnS0 & so!ution, fo!!o-ed +y 1 *! a!4a!i-iodide-a;ide rea,ent# Su+*er,e .i.ette ti.s in sa*.!e -hen addin, rea,ents# Rinse ti.s -e!! +et-een uses# Sto..er carefu!!y to e3c!ude air +u++!esG *i3 +y in/ertin, +ott!e se/era! ti*es# =hen .reci.itate has sett!ed to a+out ha!f the +ott!e /o!u*e, carefu!!y re*o/e the sto..er and add 1#0 *! conc# su!furic acid# Re-sto..er and *i3 +y ,ent!e in/ersion unti! the iodine is unifor*!y distri+uted throu,hout the +ott!e# Transfer 20) *! of sa*.!e into a -hite 500 *! cassero!e dish and titrate -ith 0#02502 sodiu* thiosu!fate to a .a!e stra- co!or# dd 1-2 *! of starch so!ution and continue to titrate to first disa..earance of the +!ue co!or# 5200 *! of ori,ina! di!ution -ater is equa! to 20) *! of di!ution -ater .!us rea,ents#7

5#

6. Titrate t-o of the three sa*.!es# Resu!ts shou!d +e -ithin 0#1 *' if usin, a +uret -ith incre*ents of 0#05 *'# "a!i+rate the DO .ro+e -ith the third +ott!e#

BOD - 3

Standardi9ation of DO meter 0 Air calibrations #emperature ( !) 15 19 11 1% 1$ 24 ambient 21 22 2) 2& 25

!alibration The =in4!er titration is the *ost accurate *ethod for standardi;in, a DO *eter# :f another *ethod is used, it is su,,ested that the ca!i+ration +e chec4ed a,ainst a =in4!er titration occasiona!!y# :f a *eter is air ca!i+rated, the readin, *ust +e corrected for at*os.heric .ressure# This is +est done -ith a +aro*eter 4e.t in the !a+, +ut another source of this infor*ation is a !oca! air.ort or ne-s station## t*os.heric .ressure readin,s o+tained fro* an air.ort are ,enera!!y corrected for sea !e/e!, and *ust +e re-corrected for actua! a!titude# :f you use a DO *eter and .ro+e, it is .erha.s easiest if you ca!i+rate accordin, to the *anufacturerHs instructions# There are t-o ty.es of o3y,en .ro+es a/ai!a+!e: one of -hich e*.!oys a ca!i+ration +ased on -ater saturated air, and another +ased on air-saturated -ater# The -ater saturated air .rocedure in/o!/es storin, the e!ectrode in a sea!ed BOD +ott!e containin, a *ini*a! a*ount of -ater# 8or the air-saturated -ater ca!i+ration .rocedure, you either /i,orous!y sha4e the so!ution or +u++!e air throu,h it# There are, ho-e/er, so*e conce.ts you shou!d +e fa*i!iar -ith to o+tain the +est qua!ity resu!ts# 1. The +est -ay to ensure data qua!ity is to ca!i+rate a,ainst a 4no-n standard# One -ay to do this is to use a sa*.!e of di!ution -ater -hich has +een saturated -ith o3y,en# By 4no-in, the te*.erature of the di!ution -ater, and that it is a saturated so!ution, you then 4no- -hat your *eter shou!d read for ca!i+ration# The ta+!e a+o/e is an a++re/iated !ist of o3y,en saturation !e/e!s /ersus te*.erature# ssu*in, your di!ution -ater is at 20 ", if you o+tain a *easured /a!ue su+stantia!!y !ess than $#0 *,6', you -i!! not ha/e an accurate ca!i+ration# 2. So*e *eters a!so a!!o- the ;ero ca!i+ration standard# By addin, an o3y,en sca/en,er 5e#,#, an e3cess a*ount of sodiu* su!fite7 to a sa*.!e of di!ution -ater, you can o+tain a sa*.!e -hich /irtua!!y contains ;ero o3y,en#

#heoretical aximum Oxygen Solubility (mg,-) 10#0%& $#%10 $#995 $#&91 $#219 <(4<2 %#$15 %#1&) %#51% %#&1% %#29)

BOD - 5

Pretreatment of !hlorinated BOD Samples ;eagents:

1# 2# )# Acetic acid solution6 /=/: dd 500 *!# of concentrated acetic acid to 500 *! of disti!!ed -ater# Potassium 7odide Solution: Disso!/e 10 ,ra*s 0: in a 100 *! /o!u*etric f!as4# Brin, to /o!u*e -ith disti!!ed -ater# Sodium Sulfite Solution6 4(4254N: Disso!/e 1#515 ,ra*s anhydrous 2 Brin, to /o!u*e -ith disti!!ed -ater#
2OT>: This so!ution is not sta+!e and *ust +e .re.ared dai!y#
2

SO) in a 1,000 *! /o!u*etric f!as4#

&#

Starch 7ndicator Solution ("or Analysis &ith 7odine) : Pre.are an e*u!sion of 5 , so!u+!e starch in a *ortar or +ea4er -ith a s*a!! a*ount of disti!!ed -ater# Pour this e*u!sion into 1 ' of +oi!in, -ater, a!!o- to +oi! a fe*inutes, and !et sett!e o/erni,ht# Bse the c!ear su.ernate# This so!ution *ay +e .reser/ed +y the addition of 1#25 , sa!icy!ic acid6' and stora,e at &"#

Procedure: 1# "onduct a ch!orine residua! ana!ysis on a .ortion of the sa*.!e co!!ected . Potassiu iodide:starch paper can be used as a #uic1 #ualitative test for residual chlorine # :f no residua! is found, .roceed -ith the BOD ana!ysis uti!i;in, seeded di!ution -ater# :f a residua! is found, .roceed -ith the fo!!o-in, ste.s +efore initiatin, the BOD test# Deter*ination of ..ro.riate Do!u*e of Sodiu* Su!fite a# +# c# d# e# f# )# O+tain a 200 *! .ortion of the sa*.!e to +e tested# dd 10 *! of 1I1 acetic acid so!ution dd 10 *! of .otassiu* iodine so!ution dd 2 *! starch Titrate -ith 0#02502 sodiu* su!fite# The end .oint has +een reached -hen a c!ear co!or .ersists after co*.!ete *i3in,# Measure /o!u*e of 0#02502 sodiu* su!fite used#

2#

Sa*.!e Pretreat*ent a# +# c# d# O+tain another 200 *! .ortion of the sa*e sa*.!e used in Ste. 2# dd to the sa*.!e the sa*e /o!u*e of 0#02502 sodiu* su!fite so!ution that -as deter*ined in Ste. 2#e and *i3# Retest for residua! ch!orine after a!!o-in, the sa*.!e to stand for 10-20 *inutes# :f no residua! ch!orine is .resent, .roceed -ith the BOD ana!ysis# Sa*.!es -hich ha/e +een ch!orinated *ust +e seeded#

BOD - '

Preparation of >lucose 0 >lutamic Acid Standard (>>A)

;eagents: "ote+ glucose:gluta ic acid solution can be purchased co concentrations are e#ual to .&3 g:- each. 1# 2# Rea,ent ,rade ,!ucose Rea,ent ,rade ,!uta*ic acid

ercially! but needs to be preapred usch that the ;;A

Procedure: 1# 2# Dry rea,ent ,rade ,!ucose and ,!uta*ic acid at 10) " for 1 hour and coo! for one hour in the desiccator# Disso!/e 150 *, 50#150 ,7 of ,!ucose and 150 *, 50#150 ,7 of ,!uta*ic acid in disti!!ed -ater and +rin, u. to 1 '#
2ote:This so!ution -i!! +eco*e conta*inated quic4!y and *ust +e used i**ediate!y un!ess the fo!!o-in, is done# P!ace into each of se/era! *i!4 di!ution +ott!es or ca..ed test tu+es the quantity of the JJ standard -hich is used in one day# Sea! the +ott!es and steri!i;e the*# These steri!i;ed .ortions can then +e coo!ed and stored at &"# =hen a 4no-n standard is run, 9 *! of JJ standard fro* one of the sea!ed6steri!i;ed containers is added to each BOD5 +ott!e and the +ott!es are fi!!ed )6& fu!! -ith di!ution -ater# 5This is critica!K 1$% )0#5 *, O3y,en6' is +ased on a 2 < di!ution of JJ 59 *'6)007# :t is i*.ortant not to use *u!ti.!e di!utions -hich use other than 9 *'7# Seed is then added and the +ott!e is fi!!ed -ith di!ution -ater# These +ott!es are incu+ated and BOD is deter*ined si*i!ar to sa*.!e +ott!es#

)#

The acce.ta+!e BOD5 /a!ue of the standard is 1$% )0#5 *,6!# :f the ca!cu!ated resu!t fa!!s outside this ran,e the cause of the .ro+!e* *ust +e identified# Sa*.!e resu!ts o+tained usin, the sa*e seed or di!ution -ater as the standard *ust +e qua!ified# Once the .ro+!e* is corrected another 4no-n shou!d +e set u. i**ediate!y#

BOD - )

BOD Seeding Procedure

Preparation of Seed: 1# "o!!ect a ra- inf!uent ,ra+ sa*.!e the day +efore .erfor*in, the test# 5f the influent contains significant industrial loading! settled i0ed li#uor ay provide a better seed than raw influent. 5f used for seed! settled i0ed li#uor does not need to be incubated at 23 % overnight. Seed can also be co ercially obtained. There are at least two products widely in use+ BioSeed ! and PolySeed . 2# P!ace sa*.!e in incu+ator 520"7 o/erni,ht#

Preparation of Seed !ontrols: Table ( gives general directions for deter ining the a ount of seed to add to seed controls and sa ples. 1# Ta4e the incu+ated ra- inf!uent sa*.!e out of the incu+ator -- DO 2OT M:L# 2# )# &# Pi.et ), 5, and 1 *! of the c!ear su.ernatant into three BOD +ott!es res.ecti/e!y# Bse other /o!u*es of su.ernatant +ased on the stren,th of your syste*# Mou MBST use at !east t-o different di!utions# 8i!! these three +ott!es -ith BOD di!ution -ater# Deter*ine the initia! disso!/ed o3y,en on each of the three +ott!es#

Preparation of Seeded BOD Samples : 1# 8i!! the +ott!es a..ro3i*ate!y 16) - 162 -ith di!ution -ater# 2# )# &# 5# Pi.et 2 *! of the su.ernatant into each of the BOD sa*.!e +ott!es that -i!! require seedin,# dd the a..ro.riate a*ount of sa*.!e to each of the +ott!es# "o*.!ete the fi!!in, of the BOD +ott!es -ith di!ution -ater# Deter*ine the initia! disso!/ed o3y,en 5:DO7 on each of the +ott!es#

!alculation of Seed !orrection: 1# Deter*ine the 5 day DO concentration on each of the seed contro!s# 2# )# &# Bse the sa*e ru!e for DO de.!etion as in a!! other BODs 5at !east 2#0 *,6' DO de.!etion and at !east 1#0 *,6' residua! DO 5after 5 days7 5Standard *ethods! .(th edition77# :f none of the +ott!es attain a .ro.er de.!etion, adNust the a*ount of seed addition accordin,!y in su+sequent tests# 8or each seed contro! di!ution, the *, DO used .er *! seed C 5:DO - DO5 for seed contro!7 *! seed in seed contro! :f t-o seed contro!s *eet the DO de.!etion criteria, ca!cu!ate the a/era,e *, DO de.!eted6*' seed# Seed correction C 5*, DO6 *! seed in seed contro!7 3 5*! seed added to sa*.!es O7
O :f the seed is di!uted +efore it is added to the sa*.!e +ott!es, the *! of the di!uted seed added to the sa*.!e +ott!e *ust +e *u!ti.!ied +y a di!ution factor# 5>3# :f 10 *! seed I $0 *! -ater are *i3ed to .roduce the seedin, *ateria!, the di!ution factor is 1610#7

5# 9#

1#

:f the seed correction does not fa!! in the ran,e of 0#9-1#0, +ut the seed contro!s *et the DO de.!etion criteria, the a*ount of seed used in the sa*.!e +ott!es -i!! ha/e to +e adNusted in su+sequent tests#

!alculation of BOD in sample : BOD5 C BOD *,6! C P5:DO -DO57 - seed correctionQ 3 di!ution factor @ di!ution factor C )00 BOD - ?

sa*.!e si;e 5*'7 #able ? 0 BOD S++D D7-@#7ON >@7D+-7N+S 517 >sti*ated BOD of seed )0 50 100 150 250 500 527 Di!utions for Seed "ontro! 15, 25, 50 15, 25, 50 5, 10, 15 5, 10, 15 ), 5, 10 1, ), 5 5)7 A *' seed .er BOD +ott!e 9 - 10 &-9 2-) 1-2 1 0#5 5&7 A *' di!uted seed 510 *' seed I $0 *' -ater7 2 2 2 2 9 - 10 5

:f the BOD of the seed is 150 *,6' or !ess, the seed *ay +e added direct!y to the BOD sa*.!es -ithout di!ution# :f di!ution is necessary, use /o!u*es noted in co!u*n 5&7# Set u. the seed contro! di!utions as sho-n in co!u*n 527# Pre.are seed contro!s -ith seed at fu!! stren,th# Seed !orrection Sample !alculation:
S++D !ON#;OBO##-+

7DO

DO5

D+P-+#7ON

A mS++D 7N BO##-+ )0 20 10

mg DO,mS++D -#-0#)& 0#&1

%#5 B "
2OT>: Bott!e

0#) 1#9 &#)

%#2 9#% &#1

%#& %#&

is not used due to the insufficient fina! DO# There *ust +e a residua! DO of at !east 1#0 *,6' after 5 days#

(0.34

I 0#&1762 C 0#)15 *, DO6*! seed

:f 2 *! undi!uted seed added to each sa*.!e +ott!e, seed correction C 50#)15 *, DO6*! seed752 *! seed7 C 0#15 *, DO

BOD - <

8aci!ity 2a*e: RRRRRRRRRRRRR

BOD5 Benchsheet
Sample -ocation 5s.ecific7 Sample #ype 5,ra+, 33 hr "o*.#, etc#7 RaRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 8ina! RRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRR RRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRR RRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRR RRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Sa*.!e Date: RRRRRRRRRRRRRRRR "o!!ected +y: RRRRRRRRRRRRRRRR Sa*.!es :2 Date: RRRRRRRRRR Ti*e: RRRRRRRRR Roo* Te*. 5"7 RRRRRRRRRR Baro*etric .ressure RRRRRRRRRR Test Date: na!yst: Sa*.!es OBT Roo* Te*. 5"7 Baro*etric .ressure :nitia! DO 5*,6'7 8ina! DO 5*,6'7 RRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRR Date: RRRRRRRRRR Ti*e:RRRRRRRRRR RRRRRRRRRRRRRRR RRRRRRRRRRRRRRR De.!etion 5*,6'7 "o**ents
De.!etion *ust +e !ess than 0#2 *,6'

Bott!e A B!an4 Seeded B!an4 Seed source: RRRRRRRRRRRRRRR Sa*.!e *'s

Sa*.!e *'s

Seed "orrection factor 5S"87:

Seed *'s

RRRRRRRRRRRR Di!ution factor

8C)006

Sa*.!e

Bott!e A

added

:nitia! DO B

8ina! DO "

DO
de.!etion

S"8 >

BOD5 *,6'
8 3 5D->7

/era,e

BOD5

DCB-"

Ra8ina! JJ
Re.!icate ofRRRRRR

/era,e on!y those /a!ues -hich are associated -ith a de.!etion of at !east 2 *,6' and a fina! DO 1 *,6'# BOD5 *,6' C P 5B-"7->Q 3 8

"a!cu!ation C

'a+ >qui.*ent Maintenance and "a!i+ration 'o,

Month RRRRRRRRRRRR Mear RRRRRRRR
Date

na!yst :ntia!s

Sa*.!er

Te*.# 5"7

Refri,# Te*.# 5"7

TSS O/en Te*. "

BOD
:ncu+ator

8eca!
:ncu+ator

Te*. "

Te*. "

.( Meter +uffers

BOD

Baro*eter

readin,

BOD Roo*

Te*. "

1 2 ) & 5 9 1 % $ 10 11 12 1) 1& 15 19 11 1% 1$ 20 21 22 2) 2& 25 29 21 2% 2$ )0 )1 Month!y Ba!ance "hec4 Month!y Ba!ance "hec4 embrane changes: DO .ro+e RRRRRRRRRRRRRRR RRRRRRRRRRRRRRR RRRRRRRRRRRRRRR
-ei,ht in *i!!i,ra* ran,e: ctua! -ei,ht5*,7:RRRRRRRRRR Measured -ei,ht5*,7 RRRRRRRRRR -ei,ht in ,ra* ran,e: ctua! -ei,ht 5,7:RRRRRRRRRR Measured -ei,ht 5,7 RRRRRRRRRR

**onia .ro+e RRRRRRRRRRRRRR RRRRRRRRRRRRRR RRRRRRRRRRRRRR

Other .ro+e RRRRRRRRRRRRRRR RRRRRRRRRRRRRRR RRRRRRRRRRRRRRR

TSS Oven should be .3,-.3& % BOD 5ncubator should be 23 . % Sa pler and <efrigerator Te perature should not e0ceed = % $and sa ples should not be fro>en' p8 calibration should be done with either a = and / buffer or a / and .3 buffer! depending on sa ple p8 range. Once calibrated! one of the two buffers should be re-chec1ed as if it was a sa ple. The easured p8 should be within 3.. p8 unit of actual p8.

!orre c t i $ e Acti o n "or m

QC failures
5ha t B! ty p e failur e is in$ol $ e d 8 RRR+!an4 RRR4no-n stan d a r d RRRca!i+ra tion RRR*atri3 s.i4e RRRre.!icat e RRR+!ind RRRother Blan : : -hat is the 'ODS RRRRR =hat !e/e! -as det e c t e d in the +!an4S RRRRRRR Spi: e s , r e p l i c a t e s : =hat are the acce . t a n c e criteriaS RRRRRR Mour res u!tS RRRRRRR :f a *a tri3 s.i4e: :s this a *atri3 interfer e n c e S RRRR (o- do you 4no- thatS RRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR Cno& n st a n d a r d s , b l i n d s : True Da!ue: RRRRR cce.t a n c e criteriaS RRRRRR Mour resu!tS RRRRRR Other .ertin e n t infor* a tio nRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R

Other problems

(equipment malfunctions, etc.) Sy m p t o m ( s ) 5 ho- did you 4no- so* e t hi n , -as -ron, S 7:RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R

Corrective Action Taken

'ist any acti/ities or chec4s you .erfor *e d to identify the sourc e and reso!/e the .ro+!e *#

ction6"he c4 Perfor * e d DateRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR

=hat did you conc!ud e S RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR RRRRRRRRRRRRRRRRRRRRRRRRRRRRRR

:nitia!s RRRRRR RRRRRR RRRRRR RRRRRR RRRRRR RRRRRRR RRRRRRR RRRRRRR RRRRRRR RRRRRRR

Resolution Date !!!!!!!!!

Brief!y docu * e n t ho- you 4no- this .ro+!e * has +ee n correct e d # =hat cha n, e s ha/e you *a d e to .re/e n t it fro* recurrin,S

RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR R