Data File D:\A_RAZAK...AZAKAGILENT HPLC 1200 SERIESAA1 2014-01-15 13-51-02\160114A000016.

D
Sample Name: std 1nmol
=====================================================================
Acq. Operator
: A_Razak
Seq. Line : 16
Acq. Instrument : Agilent HPLC 1200 Series
Location : Vial 15
Injection Date : 1/15/2014 2:09:19 PM
Inj :
1
Inj Volume : Inj prog
Acq. Method
: D:\A_Razak\Farmasi\Haruan\SYED\A_RazakAgilent HPLC 1200 SeriesAA1 2014-01-15
13-51-02\AA.M
Last changed
: 1/15/2014 1:49:43 PM by A_Razak
Analysis Method : D:\A_RAZAK\FARMASI\HARUAN\SYED\A_RAZAKAGILENT HPLC 1200 SERIESAA1 2014-01-15
13-51-02\160114A000016.D\DA.M (AA.M)
Last changed
: 1/15/2014 2:17:14 PM by A_Razak
(modified after loading)
Sample Info
: Acid amino method
ZORBAX Eclipse AAA 4.6X150mm,3.5um, 30min
Auto injector, 40C, 2ml/min.

Agilent HPLC 1200 Series 1/15/2014 2:17:17 PM A_Razak

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mAU

20

17.5

15

12.5

10

7.5

2.5

2.070

ASPARTATE

GLUTAMATE

6
6.675

7.562

SERINE

HISTIDINE

7.875
8.081

GLYCINE
THREONINE

8.353
8.661
9.262

ARGININE

ALANINE

10
10.394

TYROSINE

CYSTINE
12

11.781

12.101

12.233
12.426

14

13.599
13.801
14.408

VALINE
METHIONINE

PHYNILALANINE
ISOLEUCINE
LEUCINE
14.840

LYSINE

16
18 min

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DAD1 A, Sig=338,10 Ref=390,20 (D:\A_RAZAK...LENT HPLC 1200 SERIESAA1 2014-01-15 13-51-02\160114A000016.D)

4
4.195

7.565

8.663

9.257

10

10.394

11.781
12

12.101
12.229
12.425

13.065

14

14.407

14.840

15.236

16

17.210

DAD1 B, Sig=262,16 Ref=324,8 (D:\A_RAZAK...ILENT HPLC 1200 SERIESAA1 2014-01-15 13-51-02\160114A000016.D)

6.676

mAU

3 of 5

18 min

Page

40

35

30

25

PROLINE

17.951

20

3.917
4

13.510

13.601
13.797

8.083
7.876

15

10

2.068

Data File D:\A_RAZAK...AZAKAGILENT HPLC 1200 SERIESAA1 2014-01-15 13-51-02\160114A000016.D

Sample Name: std 1nmol
=====================================================================
Area Percent Report
=====================================================================
Sorted By
:
Signal
Multiplier
:
1.0000
Dilution
:
1.0000
Use Multiplier & Dilution Factor with ISTDs
Signal 1: DAD1 A, Sig=338,10 Ref=390,20
Peak RetTime Type Width
Area
Height
Area
#
[min]
[min]
[mAU*s]
[mAU]
%
----|-------|----|-------|----------|----------|--------|
1
2.070 BB
0.1311
81.53557
9.79894
5.8445
2
4.195 BB
0.1103
73.71957
9.52364
5.2842
3
6.675 BB
0.0929
82.64089
12.53663
5.9237
4
7.562 BB
0.0879
40.66531
6.59627
2.9149
5
7.875 BV
0.0930
82.12371
12.43989
5.8866
6
8.081 VV
0.0953
84.03557
12.36013
6.0237
7
8.353 VB
0.0872
12.63564
2.01368
0.9057
8
8.661 BB
0.0893
83.32346
13.25737
5.9726
9
9.262 BB
0.0961
85.02033
12.38910
6.0942
10 10.394 BB
0.0928
78.14758
11.86696
5.6016
11 11.781 BB
0.0890 131.09550
20.95575
9.3969
12 12.101 BV
0.1010
10.10169
1.53033
0.7241
13 12.233 VV
0.0986
81.79730
11.84532
5.8632
14 12.426 VB
0.1031
89.13430
11.94083
6.3891
15 13.599 BV
0.0989
77.00696
11.10559
5.5199
16 13.801 VB
0.1039
85.33498
11.32883
6.1168
17 14.408 BB
0.1042
83.75376
11.33234
6.0035
18 14.840 BB
0.0960 108.56626
16.22927
7.7820
19 23.895 BB
0.0825
24.45310
4.04787
1.7528
Totals :

1395.09148

203.09875

Signal 2: DAD1 B, Sig=262,16 Ref=324,8

Peak RetTime Type Width
Area
Height
Area
#
[min]
[min]
[mAU*s]
[mAU]
%
----|-------|----|-------|----------|----------|--------|
1
0.904 VB
0.0693
43.17530
9.44574
1.2688
2
1.130 BV
0.0663
9.00672
2.08970
0.2647
3
1.247 VB
0.0605
47.19201
11.85061
1.3868
4
2.068 BB
0.1257
12.98613
1.55102
0.3816
5
3.917 BB
0.1351 379.53802
39.87288 11.1532
6
6.676 BB
0.0918
11.69550
1.80048
0.3437
7
7.565 BV
0.0928
9.97023
1.51470
0.2930
8
7.876 VV
0.1108
19.04790
2.29481
0.5597
9
8.083 VV
0.1136
28.47913
3.40255
0.8369
10
8.663 BB
0.1411
36.31375
3.38727
1.0671
11
9.257 BB
0.1858
70.24327
4.88307
2.0642
12 10.394 BB
0.0903
37.39241
5.86938
1.0988
13 11.781 BB
0.0997
54.99199
7.66749
1.6160
14 12.101 BV
0.1127
19.50719
2.56646
0.5732
15 12.229 VV
0.1019
27.76818
3.86259
0.8160
16 12.425 VB
0.1053
19.63098
2.50966
0.5769
17 13.065 BB
0.1326
37.44826
3.87879
1.1005
Agilent HPLC 1200 Series 1/15/2014 2:17:17 PM A_Razak

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Data File D:\A_RAZAK...AZAKAGILENT HPLC 1200 SERIESAA1 2014-01-15 13-51-02\160114A000016.D

Sample Name: std 1nmol
Peak RetTime Type Width
Area
Height
Area
#
[min]
[min]
[mAU*s]
[mAU]
%
----|-------|----|-------|----------|----------|--------|
18 13.510 BV
0.0694
28.87965
6.31257
0.8487
19 13.601 VV
0.1200
78.46895
9.33699
2.3059
20 13.797 VB
0.1102
34.47940
4.26912
1.0132
21 14.407 BB
0.1047
22.85285
3.07670
0.6716
22 14.840 BB
0.0984
16.85554
2.44649
0.4953
23 15.236 BB
0.1807
19.98549
1.70565
0.5873
24 17.210 BB
0.1220
10.44643
1.24374
0.3070
25 17.951 BB
0.1648
97.81248
9.14063
2.8743
26 19.455 BV
0.0674 676.36560 142.38953 19.8758
27 19.587 VV
0.0788 1189.33606 207.68481 34.9500
28 19.907 VV
0.1333
68.20435
7.02335
2.0043
29 20.238 VB
0.0885 155.88272
28.21565
4.5808
30 20.579 BB
0.1241
81.86804
9.73651
2.4058
31 21.838 BB
0.0846
8.37251
1.42325
0.2460
32 23.894 BB
0.0852
48.76583
7.77459
1.4330
Totals :

3402.96288

550.22676

=====================================================================
*** End of Report ***

Agilent HPLC 1200 Series 1/15/2014 2:17:17 PM A_Razak

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