Rebecca Dover Microbiology 210, Section 00425 April 2009
Mixed Culture Unknown Identification ReportIntroduction:
The objective of this experiment is to identify the organisms in a mixed culture of twounknown bacteria. One organism is known to be a gram positive, and the other a gramnegative. It is important to isolate colonies of individual organisms and obtain a pure cultureof each in order to determine the identity of the organisms. With a pure culture, it is simple toinoculate the culture into media that identify the biochemical properties of each organism.Comparing the known results of particular organisms with that of the unknown culture canidentify the genus and species of the bacteria in the culture. Using this knowledge, the identityof bacteria in unknown culture #1 will be revealed in this report, based on this information,which directed the tests that were performed. The possible Gram positive species were
Clostridium perfringes, Corynebacterium xerosis, Enterococcus faecalis, Staphylococcusaureus, or Staphylococcus epidermidis.
The potential Gram negative species were
Enterobacter aerogenes, Moraxella catarrhalis, Neisseria flavescens, Pseudomonasaeruginosa, or Proteus vulgaris.
Materials and Methods:
On the first day, after receiving the tube containing the two bacteria, a Gram stain was