Professional Documents
Culture Documents
Filter paper
Filter paper was taken and was placed in the centrifuge tube.
Sonicator and cold water bath. - The samples were sonicated for 15 minutes.
The samples were put in room temperature for 30 minutes. Then shake well.
Ambient temperature
cuvette
90% Acetone
Chlorophyll-a were calculated using Lorenzo (1967) equation and trophic state index (TSI) were calculated using Carlsons TSI