1.Sampling of biological speciments
--Invasive methods: Blood, spinal fluid, tissue biopsy.--Non-invasive methods: Urine, salive, feces, expired air
Measurement of drug concentration in the blood, serum or plasma is the most directapproach to assess the pharmacokinetics of drug in body.
2.Significance of measuring plasma drug concentration
The intensity of the pharmacologic or toxic effect of a drug is oftenrelated to theconcentrationof the drug at the receptor sites.
Monitoring the concentration of drugs in the blood or plasma ascertainsthat the calculated dose actually delivers the plasma level required for therapeutic effect.
Measurement of drug concentration