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ELISA is defined asan“immunological”test that usesenzyme linked antiglobulin & substrate  bound to an“inert surface”.
Assay-A quantitative or qualitative test of a substance (especially an ore or a drug) to determine its components; frequentlyused to test for the presence or concentration of infectious agents or antibodies etc.
 
 
ELISA
The most important Serological TestELISA is a“Serological Test”which is used for either detection of Ag or Ab.A reaction is said to be “Serological”; when Ag-Ab reactions arecarried out
in-vitro
.
This is aSerological Test.See the
in-vitro Ag-Ab
reactionbeing carried out.
This is ELISA plate.See the 96 walls onthe plate with
12X8 arrangement 
of 00

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Good yaar, keep it up, done a good work and presentation. Mhase sir khus zaale aastil.... keep it up

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