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1 Test per tube
Product catalog No: 25332-00ReaPan 3 8 G
Manufactured by
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ReaPan 3 8 G Reagent
1.INTENDED USE
The ReaPan 3 8 G reagent is a two color immunofluorescence staining reagent forthe enumeration of absolute counts of total T-lymphocytes (CD3+) and Suppressor/ cytotoxic (CD8+) T-Cells. This reagent is intended for flow cytometry basedanalysis in lyzed human whole blood samples, preferably on the Guava PCASystem
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BACKGROUND
The ReaPan 3 8 G reagent contains fluorescently labeled antibodies that bind toCD3 and CD8 antigens found on the surface of circulating leukocytes in peripheral
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blood samples.The CD3 antigen is a complex of at least six proteins known collectively as the T-cell receptor (TCR) complex. The antibody used in this reagent binds to the 20kDachain of this complex.
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The CD8 antigen is a complex consisting of two disulfide linked subunits. Theantibody used in this reagent binds to the 32KDa subunit of the complex.CD8
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 interacts with class I major histocompatibity complex molecules.Cells that are CD3+ and CD8+ are identified as suppressor/cytotoxic Tlymphocytes. Decreased CD8+CD3+ cell counts have been associated with someforms of immunodeficiency.
3.REAGENT
The ReaPan 3 8 G reagent is formulated in buffered saline with sodium azide andstabilizers. It contains R-Phycoerythrin (PE)– labeled anti-CD8 monoclonalantibody, clone LT8; R-Phycoerythrin (PE)-Dyomics 649 – labeled anti-CD3monoclonal antibody, clone UCHT1; The monoclonal antibodies used in the
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