The document contains the sequence of a pET-28a(+) vector used for bacterial protein expression. It includes features like T7 promoter, lac operator, ribosome binding site, start codon, and 6xHis tag for purification of expressed proteins. The sequence is 2811 base pairs long and contains elements necessary for protein expression and purification in E. coli.
The document contains the sequence of a pET-28a(+) vector used for bacterial protein expression. It includes features like T7 promoter, lac operator, ribosome binding site, start codon, and 6xHis tag for purification of expressed proteins. The sequence is 2811 base pairs long and contains elements necessary for protein expression and purification in E. coli.
The document contains the sequence of a pET-28a(+) vector used for bacterial protein expression. It includes features like T7 promoter, lac operator, ribosome binding site, start codon, and 6xHis tag for purification of expressed proteins. The sequence is 2811 base pairs long and contains elements necessary for protein expression and purification in E. coli.