SEM: IV (BT) SUBJECT MOLECULAR GENETICS L: 03 P: 00 T: 00

COURSE OBJECTIVES & SCOPE:

The objectives of this course are to
Understand the Central Dogma of gene e!ression and to understand the foundations of "endelian genetics and chromosomal
theor# and a!!l# these$ %ith a!!ro!riate terminolog#$ to contem!orar# conce!ts in genetics&
Understand the redundant and universal 'ualities of the genetic code and ho% it is used to determine the amino acid se'uence
of a !ol#!e!tide&
To understand the !rocesses of transcri!tion and translation in both !ro(ar#otes and eu(ar#otes at the molecular level and ho%
!ro(ar#otes control their gene e!ression through !ositive and negative regulator# mechanisms&
REFERENCES:
)eference * Tet Boo(s+ ,
-&) "olecular Biolog#. David /reifelder$ 0arosa 1ublishing 2ouse$3nd edition (3004)
3) "icrobial 5enetics. David /reifelder$ 0arosa 1ublishing 2ouse$ 3nd edition(3004)
3) 1rinci!les of 5ene "ani!ulations. 6& B& 1rimrose$ )& "& T%#man$ )& 7& old$ Blac(%ell 6cience$8th 9dition (3003)
4) 5ene VIII. Benjamin :e%in. ;ford Univ& 1ress$ <th edition (3004)
SESSION PLAN
Unit Lecture
No.
Topic P!"nne# $"te
Con#ucte#
Re%.
T&R
'.
(.
-&
Intro#uction to Mo!ecu!"r Genetic T)e tu#* o% Structure o% Nuc!eic
Aci#:
-&D0=
3&)0=
3&T#!es of D0=
4&T#!es of )0=
i& m)0=
ii& t)0=
iii& r)0=$
)B-
3&
Denaturation and )enaturation of D0= , loss of the >uaternar# structure$
tertiar# structure$ secondar# structure$ getting the D0= to its native state&
Tm ? The melting tem!erature of the double stranded D0=&
Conversion of the strands from double to single stranded and uncoiling of
the strands&
2o% the 5C content is determined from Tm
)B-
3&
)enaturation (inetics of D0=$ Com!leit# of D0= ? Denaturation of D0=
-& 1rocedure
3& =nal#sis of the results
3& =!!lication in D0= se'uencing @ genome anal#sis
)B-
4&
7hat are Cot curves ? Cot anal#sis (reassociation of D0=)
6atellite D0= ? Tandeml# re!eating D0= non coding D0=&
601 ? single nucleotide !ol#mor!hism 6T) ? Inherited D0= in !eo!le
almost similar se'uences of D0= but var#ing in all individuals&
)B-
A&
"endelism and its etensions ?
Bohn 5regor "endel ? 2istor# and 9!eriment&
-& /-5eneration
3& /3 5eneration
)B-
8
The different :a%s 1ro!osed b# "endel
:a% of segregation
:a% of inde!endent assortment
Chromosomal basis of segregation and inde!endent assortment
)B4
C
2o% the factors li(e , :in(age$ Crossing over$ "ulti!le allelism 1leiotro!#
)ecombination are res!onsible for the three !ro!osed la%s&
)B3
<
7hat is C#togenetics ? The stud# of chromosomes and their relation %ith
inheritanceD
)B3
E
International 6#stem for 2uman Chromosome 0omenclature ? ho% this id
relevant %ith the chromosomal (ar#ot#!ing and other !arameters&
)B3
-0
The "echanisms of numerical and structural chromosomal aberrations and
ho% Chromosomal basis of se determination %or(s& 0on,chromosomal
basis of se determination&
)B3
--
7hat is "utation ? The stud# of 6omatic "utation$ germline mutationD
The various t#!es of mutations li(e
i& misense mutation
ii& !oint mutation
iii& nonsense mutation
iv& null mutation
)B-
-3
D0= )e!lication ? 7hat is D0= re!lication and %h# it is im!ortantD
9u(ar#otic D0= re!lication ? T#!es of D0= re!lication
-&Conservative
3&6emiconservative
3&Circular
)B-
-3
"echanism of D0= re!lication ? 6emi,conservative mode of D0=
re!lication,6tages
of re!lication
-&Intiation ? un%inding of the double heli$
3& 9longation , /ormation of ;(aFa(i fragments
)B-
+. -4
3& )e!lication /or( ? leading strand lagging strand$ 6#nthesis of the
daughter strands$
4& 1roof reading of the ne% daughter strands&
)B-