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1
PTOto find
Doctor GyaanBrain gym
ReaDigest 
10 of Nov2009Volume 1, Issue 2
Focus of the month Cancer 
To know the answers for‘‘Brain Gym’, log onto
readigest.reametrix.com
 
Season’s Greetings
 
We are pleased toinform that our
Dry Tri T-STAT– CD4Detection Kit
is chosen as
 
BioSpectrumBiotech Productof the Year2009*
 
*The details will beavailable in December issueof BioSpectrum
To know more about usplease visit
www.reametrix.com
Contact usReaMetrix India Pvt.Ltd, 50 B, 2nd Phase,TVS Cross, PeenyaIndustrial Area, PeenyaBangalore- 560 058India.
Phone:
+91 80 28378693
 Fax :
+91 80 4117 2451
 
Minimal Cytoplasmic Panel–
A Robust Test Design For Leukemia Immunophenotyping
 
The tube allows forSingle step staining of surface andcytoplasmic markers;Single step lysis and permeabilizationMinimal number of washes/steps anda quick time to result (<1hr);lesser variabity due to standardiza-tion of protocol across labs;dried down format to reduce reagentlossesNo additional antibody pipetting andtitration steps.It is hypothesized that by using thecytoplasmic minimal screening tube,alternate algorithms can evolve foracute leukemic blast lineage identifi-cation, thereby providing affordablepatient diagnostic options.The points below summarize somenovel features of this tube:1. Allows simultaneous staining ofcytoplasmic and surface antigens2. Takes less than one hour and re-quires no antibody titrations or pi-petting steps.3. Minimizes variability by reducingthe number of steps and allows forinter-laboratory standardization.Leukemias can be categorized as adisease that involves an unusual andmassive build up in the number of thewhite blood cells in the blood or thebone marrow. The decision for treat-ment is made by looking at the age ofthepatient,clinicalsymp-tomsandresultsfromspecificdiagnos-tic testson pa-tientblood orbone marrow samples. Two importantquestions that have to be answered tochoose the treatment and provideprognostic information for acute leuke-mic patients are:
What is the lineage of the leukemic blasts? And what spe- cific antigens do they express? 
Currently, answers to these questionsare obtained using information frommorphology, flow cytometry, cytochem-istry and cytogenetics of patient bloodor bone marrow samples (Figure). Flowcytometric immunophenotyping pro-vides key lineage and prognostic anti-gen expression information. Pres-ently, a panel of ~13-15 antibodiesis used for the flow cytometricimmunophenotyping of acute leuke-mias. Along with the other tests,the associated cost burden to thepatient is high.At ReaMetrix, we asked,
“1. How do we create standardized easy-to-use solutions for intracel- lular markers that are highly line- age specific? 2. How do we design affordable screening of acute leukemic blasts?” 
As a solution, we developed a ro-bust single tube assay for screen-ing blast lineage in less than 1 hourusing cytoplasmic markers. Thetube contains dried antibodiesagainst cytoplasmic MPO (Myeloidlineage), cytoplasmic CD22 or cyto-plasmic CD79a (B-Lymphoid), sur-face CD45 (Blast Gating) and cyto-plasmic CD3 (T-Lymphoid). Througha rational selection of antibodyclones, concentrations, fluoro-chromes, fixation and permeabili-zation agents, both surface andcytoplasmic markers can be stainedin a single tube with minimal proc-essing steps.
Figure showing Immunopheno-typing decision tree
MPO: Myeloperoxidase; NSE: Non-specific esterase; ALL: Acute lym- phoblastic leukemia; AML: Acutemyeloid leukemia
 If you would like to discontinue receiving this communication please select ‘opt out’ option at readigest.reametrix.com
Do you know?
Curcumin found in turmeric has potential anticancer effects. This stems from its ability to induce apoptosis(programed cell death) without cytotoxic effects on healthy cells. Curcumin can interfere with activity ofthe transcription factor NF-κB, that is linked to a number of inflammatory diseases including cancer. Forfurther details refer to the following publication:
Aggarwal BB, Shishodia S
. Suppression of the nuclear factor-kappaB activation pathway by spice-derived phytochemicals:reasoning for seasoning. Annals of the New York Academy of Sciences. Dec 2004;
1030
:434-41.
More interesting information on various cancers can be obtained at:1. www.cancer.gov/ 2. www.tatamemorialcentre.com/3. www.leukemia-lymphoma.org/ 4. www.cancerfoundationofindia.org/
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