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Bergey's Manual of Determinative Bacteriology

Bergey's Manual of Determinative Bacteriology

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Published by erinulat

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Published by: erinulat on Nov 17, 2009
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08/07/2013

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Identification flow charts
1
Bergey’s Manual of Determinative Bacteriology
All of the unknowns will fall into the following groups in Bergey's Manual of DeterminativeBacteriology (The pink book on the shelf in the laboratory).
GROUP 4
Description:
Gram Negative, Aerobic/Microaerophilic rods and cocci
Key differences are:
pigments/fluorescent, motility, growth requirements, denitrification,morphology, and oxidase, read Genera descriptions
Examples:
Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter 
 
GROUP
 
5
Description:
Facultatively Anaerobic Gram negative rods
Key differences are:
growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions
Examples
: Family Enterobacteriaceae and Vibrionaceae
GROUP 17
Description:
Gram-Positive Cocci
Key differences are:
oxygen requirements, morph., growth requirements (45°C and supplements),read Genera descriptions
Examples:
Micrococcus, Staphylococcus, Streptococcus, Enterococcus, Lactococcus, Aerococcus
 
GROUP
 
18
Description:
Endospore-Forming Gram positive rods and cocci
Key differences are:
oxygen requirements, motility, morph, catalase
Examples:
Bacillus, Clostridium
GROUP 19
Description:
Regular, Nonsporlating Gram positive rods
Key differences are:
morph., oxygen require, catalase
Examples:
Lactobacillus, Listeria
GROUP 20
Description:
Irregular, Nonsporlating Gram-positive rods
Key differences are:
catalase, motility, morph., read Genera descriptions
Examples:
Actinomyces, Corynebacterium, Arthrobacter, Propionibacterium
 
GROUP 21
Description:
Weakly Gram-Positive Nonsporlating Acid Fast Slender Rods
Key differences are:
acid fast, growth
Examples:
Mycobacterium
 
 
Identification flow charts
2
Differentiation via Gram stains and cell morphology.
Gram Stain & MorphologicalFlowchart
Some Examples
Gram Positive
CocciBacilli
Round in clusters& tetrads:
StaphylococcusMicrococcus Peptococcus
Oval shape inChains:
Streptococcus Peptostreptococcus Enterococcus
Club-shaped and/or inpalisades:
Corynebacterium Listeria ErysipelothrixMycobacterium (Acid Fast) Propionobacterium
Spore-bearing,large, uniform:
 BacillusClostridium
Branching rudi-mentry or absent:
 Erysipelothrix Lactobacillus Eubacterium
Tiny-cocco-bacillary:
 Brucella Bordetella Bacteroides NeisseriaVeillonella
Exaggeratedpointed ends:
 Fusobacteriumnucleatum
Extensivebranching:
 Actinomyces Arachnia Nocardia
(partiallyacid-fast)
Streptomyces
Curved,comma-shaped:
VibrioCampylobacter Spirillium
Pleomorphiccoccobacillary& filamentous:
 Haemophilus Bacteroides Pasteurella Francisella Actinobacillus EikenellaCardiobacterium Flavobacterium
UniformlyBacillary:
 Enterobacteriaceae Pseudomonas Aeromonas AlcaligenesChromobacterium
Filamentous
Gram Negative
CocciBacilli
Medium-sizedcocco-bacillary:
 Acinetobacter Moraxella
Coiled &sphero-plastic:
Streptobacillus Fusobacterium
 
Identification flow charts
3
Gram Positive Rods ID Flowchart
 Bacillus
spp.
Clostrididium
spp.
Corynebacterium
spp.
 Lactobacillus
spp.
Mycobacterium
spp.
 Bacillus
spp.
Clostridium
spp
Mycobacterium smegmatisCorynebacterium
spp.
 Lactobacillus
spp.
Mycobacterium
spp.
Corynebacterium
spp
 Lactobacillus
spp.
Clostridium
spp.
You sure you have this?Go to Bergey's.
 Bacillus
spp.
See separate flowchart.
Corynebacterium
spp.
Corynebacterium kutsceri Lactobacillus
spp.
 Lactobacillus delbrueckiiCorynebacterium xerosis Lactobacillus casei Lactobacillus delbrueckii Lactobacillus casei Lactobacillus fermenti
Gram Positive Rods
Spore FormingAcid FastStrict AnaerobesCatalaseStarch HydrolysisGlucose Ferm. Activity
Acid & GasAcid
Mannitol+------+++++

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