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radioimmunoassay

radioimmunoassay

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its a important ppt
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Categories:Types, Research
Published by: sr_bakeg on Nov 18, 2009
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05/22/2013

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Radioimmunoassay &Enzyme Linked Immunosorbent Assay 
M K Unnikrishnan [Aug 2006]
 
 Radioimmunoassay 
Principle: Uses an immune reaction [Antigen –Antibody reaction] to estimate a ligand
Ag + Ag* + Ab
AgAb + Ag*Ab + Ag +Ab*
Unbound Ag* and Ag washed outRadioactivity of bound residue measuredLigand conc is inversely related to radioactivity
[Ag : ligand to be measured ; Ag* radiolabelledligand]
 
vanagesDisadvantages of RIA
Advantages
Highly specific: Immune reactions are specificHigh sensitivity : Immune reactions are sensitive
Disadvantages
Radiation hazards: Uses radiolabelled reagentsRequires specially trained personsLabs require special license to handle radioactivematerialRequires special arrangements for
Requisition, storage of radioactive materialradioactive waste disposal.

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