Christi Barr Block 421 November 2009
Cellular Respiration and Oxygen Consumed in Peas
Aspect 1
The purpose of this lab is to measure the oxygen consumption during germination inorder to measure the cellular respiration rate in both germinating and non-germinating peas at different temperatures. We are trying to see how temperature affects cellular respiration among both categories of peas. The independent variable is the time of eachreading. The dependent variable is the volume of oxygen consumed. The control is therespirometer with glass beads in it.
Hypothesis
Knowing that all living things undergo cellular respiration, I predict that the germinating peas will consume the greatest amount of oxygen, while the dry peas will consume lessand the glass beads will consume none. Also, I predict that higher temperatures willincrease the rate of oxygen consumption.
Aspect 2, 3
Materials
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100-mL graduated cylinder
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Dried peas
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Germinating peas
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Glass beads
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Ice
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2 tubs of water (one at roomtemperature and one at 10 °C)
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KOH
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Dropper
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Absorbent cotton
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Nonabsorbent cotton
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Timer
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Thermometer
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Paper towels
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6 vials
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6 Stoppers
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12 washers
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6 pipettes
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Food coloringProcedure1.Set up two tubs of water, one at room temperature and the other at 10 °C (add iceas needed).2.Fill a 100-mL granulated cylinder with 50 mL of water and place 25 germinating peas in it to determine the amount of water displaced and then place the peasaside.3.Refill the cylinder and add 25 dry peas in, and then continue to add glass beadsuntil the volume is equal to that of the germinating peas.4.Once again, refill the cylinder and add only glass beads until the same volume isreached.5.Repeat steps 2-4 again to obtain the vials for the other tub of water.6.To assemble the respirometer, put a small piece of absorbent cotton in the bottomof each vial and soak it with KOH.7.Then place a wad of dry nonabsorbent cotton on top of that.8.Place the first set of germinating peas, dry peas and beads, and beads in each vial.- 1 -
Christi Barr Block 421 November 20099.Insert the stoppers and place two washers around the pipettes.10.Repeat steps 8 and 9 with the second set of peas.11.Create a sling connecting each side of the water tubs to rest the pipettes on.12.Place the vials in the water with the pipettes on the sling for 7 minutes to allowfor equilibration.13.After 7 minutes, place a drop of food coloring at the end of each pipette and lower all into the water at the same time.14.Wait three more minutes and then collect the first set of data as the initial positionof water 15.Every 5 minutes for 10 minutes take readings of the water position in each pipette.
Aspect 4
Measurement of O
2
Consumption by Soaked and Dry Pea SeedsTemp(°C)Time(min)Beads AloneGerminating PeasDry Peas and BeadsReadingat time x(mL)Difference(mL)Readingat timex(mL)Difference(mL)Correcteddifference(mL)Readingat timex(mL)Difference(mL)CorrectedDifference(mL)200.05.05.065.06.01.1.05.04.060.0910.06.01.15.1.09.060.0915.06.01.18.13.12.060.0920.06.01.21.16.15.060.09110.12.14.15.1.02.18.04.02.10.0810.07.05.20.06.01.07.03.0215.05.07.22.08.01.05.05.0220.06.06.24.1.04.04.060Uncertainty: +/- .01 mL+/- 1°CDifference: (initial reading at time 0) – (reading at time X)Corrected Difference: (initial pea seed reading at time 0 – pea seed reading at time X) – (initial bead reading at time 0 – bead reading at time X)
Aspect 5
Rate of Oxygen Consumed per MinuteConditionCalculationRate (mL O
2
/minute)Germinating Peas/ 11°C|(.12-.14)| / 20 .001Germinating Peas/ 20°C|(.06-.05)| / 20.0005Dry Peas/ 11°C|(.12-.1)| / 20.001Dry Peas/ 20°C|(.05-.06)| / 20.0005- 2 -
Christi Barr Block 421 November 2009
Aspect 6
This graph shows the amount of oxygen consumed in each vial. From the graph, it isclear to see that the germinating peas produced much more oxygen than that of the dry peas. Also, the peas at the colder temperature seem to have worked better.- 3 -
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