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Environmental Engineering Laboratory
 By
: Ngoc Thuan Le
Total Phosphorus (T-P) - ascorbic method
Principle:
Ammonium molybdate and potassium antimonyl tartrate react in acid medium withorthophosphate to form a heteropoly acid-phosphomolybdic acid that is reduced to intensely coloredmolybdenum blue by ascorbic acid.
Apparatus
Volumetric tubes, incubator, Spectrophotometer.
Procedure
1.
Place 50ml sample with 10ml solution of K 
2
S
2
O
8
4% w/v (potassium peroxodisulfate) in100ml bottle.2.Autoclave at 120oC, 30mins
3.
Remove bottle from autoclave, take 25ml sample to the test tube.
4.
Add 2ml solution of 5V
(NH
4
)
6
Mo
7
O
24
 
(
ammonium molybdate) and 1V
 
C
6
H
8
O
7
7.2% (ascorbicacid).
5.
Keep at 20-40
o
C in incubator for 15 minutes (Don’t need to bring to final-50ml solution).
6.
Measure color photometrically at 880nm and compare with calibration curve, using distilledwater blank.
7.
Calibration curve: P-PO4 solution 0.005mg/ml (5mg/L)Concentration range: 0, 1, 4, 5, 10ml
Calculation
a:
Reagents
-
K 
2
S
2
O
8
4% w/v, dissolve 4gr K 
2
S
2
O
8
4% in 100ml solution.
-
6g of Ammonium molybdate (
(NH
4
)
6
Mo
7
O
24
.
4H
2
O, 1235.86)
+ 0.24 gr of antimonylPotassium tartrate 3-hydrate (C
8
H
4
K 
2
O
12
Sb
2
.3H
2
O, 667.87) and 300ml of H
2
O. Then,adding solution of 120ml H
2
SO
4
(2+1) (mean 2V H
2
SO
4
+1V H
2
O) + 5gr ammoniumsulfamate (NH
4
OSO
2
 NH
2
, 114.12), bring the solution to 500ml with water.
-
Ascorbic 7.2% (w/v): dissolve 7.2 g ascorbic powder in 100ml dW (prepare whenusing).
-
Working solution: 5V ammonium molybdate solution: 1V ascorbic. This mixture isstable for 4h.
-
Standard solution, dissolve 0.439gr potassium phosphate monobasic (KH
2
PO
4
, 136.09)in water to 1000ml (~ 0.1gr P-PO
4
/L, 100mg/L, dilute 20 times before using to havesol’s 5mg/L).
5010002560)/
××=
a LmgP 
of 00

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