Welcome to Scribd, the world's digital library. Read, publish, and share books and documents. See more
Download
Standard view
Full view
of .
Look up keyword
Like this
0Activity
0 of .
Results for:
No results containing your search query
P. 1
Silica Based Stationary Phase

Silica Based Stationary Phase

Ratings: (0)|Views: 4|Likes:
Published by Rinta Moon
Chromatography
Stationary Phase
Silica Based Stationery Phase: Octadecyl-bonded phases,
Butyldimethyl bonded, endcapped silica, Phenyl bonded silica, Cyanopropyl bonded, endcapped silica, Diol bonded silica, Aminopropyl bonded silica, ) Quaternary amine bonded silica with Cl – counterion,
Aluminum Oxide as Stationary Phase
Cellulose as Stationary Phase
Ion Exchange Resins
Stationery Phase used in other Chromatographic Applications:pentane, propanone and ethanol, inert solid such as silica gel (SiO2.xH2O),
alumina (Al2O3 xH2O.) or cellulose supported in a glass column
Chromatography
Stationary Phase
Silica Based Stationery Phase: Octadecyl-bonded phases,
Butyldimethyl bonded, endcapped silica, Phenyl bonded silica, Cyanopropyl bonded, endcapped silica, Diol bonded silica, Aminopropyl bonded silica, ) Quaternary amine bonded silica with Cl – counterion,
Aluminum Oxide as Stationary Phase
Cellulose as Stationary Phase
Ion Exchange Resins
Stationery Phase used in other Chromatographic Applications:pentane, propanone and ethanol, inert solid such as silica gel (SiO2.xH2O),
alumina (Al2O3 xH2O.) or cellulose supported in a glass column

More info:

Published by: Rinta Moon on Jun 21, 2014
Copyright:Traditional Copyright: All rights reserved

Availability:

Read on Scribd mobile: iPhone, iPad and Android.
download as DOCX, PDF, TXT or read online from Scribd
See more
See less

06/21/2014

pdf

text

original

 
ASSIGNMENT ON
Stationary Phase
Course name: Advanced Pharmaceutical Analysis Course code: PHRM 409
SUBMITTED TO:
Dr. Shamsun Nahar Khan Associate Professor 
 
Department of Pharmacy East West University
SUBMITTED BY:
 
Samiya Khondaker Rinta ID: 2010-3-70-048 Submission date: 9
th
 June, 2014
 
Chromatography
Chromatography is a method by which a mixture is separated by distributing its components  between two phases. It consists of a large group of separatory methods in which the components of a mixture are separated by the relative attraction of the components for a stationary phase (a solid or liquid) as a mobile phase (a liquid or gas) passes over the stationary phase (Karger, 2014).
Stationary Phase
The stationary phase is a porous solid (e.g., glass, silica, or alumina) that is packed into a glass or metal tube or that constitutes the walls of an open-tube capillary. The stationary phase remains fixed in place while the mobile phase carries the components of the mixture through the medium  being used. The stationary phase acts as a constraint on many of the components in a mixture, slowing them down to move slower than the mobile phase. The movement of the components in the mobile phase is controlled by the significance of their interactions with the mobile and/or stationary phases. Because of the differences in factors such as the solubility of certain components in the mobile phase and the strength of their affinities for the stationary phase, some components will move faster than others, thus facilitating the separation of the components within that mixture
 (
Karger, 2014). Below are discussions of some stationery phase used in HPLC.
Silica Based Stationery Phase
Some silica based stationery phases are discussed below with their structure and names of the compounds that they separate.
1) Octadecyl-bonded phases:
Amongst the numerous base-deactivated ODS phases obtained by increasing the bonding density or/and by efficient endcapping treatments, some particular stationary phases have been developed, to limit the additional interactions of basic compounds with residual silanols, to work at extreme pH or with rich water mobile phases
 
(Lesellier
et al.
, 2006).
 
i.
 
Octadecyl bonded, end capped silica Figure:
 Octadecyl bonded, end capped silica
ii.
 
Octyl bonded, endcapped silica Figure:
 Octyl bonded, endcapped silica
Compounds separated by these:
 For reversed phase extraction of nonpolar to moderately polar compounds, such as end capped silica antibiotics, barbiturates, benzodiazepines, caffeine, drugs, dyes, essential oils, fat soluble vitamins, fungicides, herbicides, pesticides, hydrocarbons,  parabens, phenols, phthalate esters, steroids, surfactants, theophylline, and water soluble vitamins (Lesellier
et al.
, 2006)..
2) Butyldimethyl bonded, endcapped silica Figure:
 Butyldimethyl bonded, endcapped silica
Compounds separated by this:
 Less hydrophobic than LC-8 or LC-18 and for extraction of  peptides and proteins.

You're Reading a Free Preview

Download
scribd
/*********** DO NOT ALTER ANYTHING BELOW THIS LINE ! ************/ var s_code=s.t();if(s_code)document.write(s_code)//-->