Schedule of Training in Diagnostic MolecularBiology Unit /2007
Day 1
-Traditional or Basic PCR: (PCR run required farther investigation), Using
ThermalCycler Machine
.
1-
Simple PCR
(one primer pair- one target).
2-
Competitive PCR
(one primer pair – several targets).
3-
RT-PCR
(
reverse transcription PCR
); convert RNA to cDNA thenPCR.
4-
Multiplex PCR
(several primer pairs- several targets).
5-
Nested PCR
(PCR run for PCR products).
6-
Arbitrarily- Primed PCR
(random primer).
Day 2-
Automated PCR (PCR run followed by automated detection)1- Automated competitive PCR (e.g.: HCV qualification andquantification, HBV quantification, HIV quantification on
CobasAmplicor Analyzer
)
Day 3
-Real Time PCR: (on-time PCR detection while amplification occurs)1- Real Time competitive PCR. (HSV 1/2 detection on
LightCyclerMachine
).
Day 4
-Automated Real Time PCR (Automated on-time detection whileamplification going on)1- Automated competitive Real Time PCR (on-time detection andquantification during the amplification); e.g.: HCV test, HBV test,and HIV test on
Cobas TaqMan 48 Analyzer
).
Day 5
1- Revision and Exam.
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